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Title:Determination of biogenic amine-forming potential of naturally occuring lactic acid bacteria (LAB) strains isolated from the grapes of Vitis vinifera cv. Refošk grape variety
Authors:Topić, Jelena (Author)
Butinar, Lorena (Author)
Bergant Marušič, Martina (Author)
Korte, Dorota (Author)
Mozetič Vodopivec, Branka (Author)
Files:This document has no files. This document may have a phisical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized (r6)
Tipology:1.12 - Published Scientific Conference Contribution Abstract
Organization:UNG - University of Nova Gorica
Abstract:Biogenic amines are naturally occurring compounds that have been reported in variety of food, such as fish, meat, cheese, vegetables, and wines. When biogenic amines are formed by microorganisms such as lactic acid bacteria (LAB) they may have adverse effect on human health. The most common biogenic amines found in foodstuff are histamine, tyramine, putrescine and cadaverine. LAB are used as starters in the production of several foods and beverages. In wines they are used in malolactic fermentation, where the conversion of malic acid to lactic acid occurs. The aim of this work was to determine biogenic amine-forming capacity of indigenous LAB strains isolated from grapes which were PCR positive and screening medium positive for biogenic amines. Three methods for the confirmation of LAB biogenic amine-forming potential were developed and compared: HPLC-DAD and TLC methods both using derivatization agent dansyl chloride and enzymatic method using horseradish peroxidase (HRP) and diamine oxidase (DAO) enzymes. The applicability of methods was tested on the three known biogenic amine-producing LAB strains: 0006, 9809, 9906 (IOEB, France). The limit of detection (LOD) of the methods was <1 mg/L for HPLC-DAD, 5 mg/L for TLC and 25 mg/L for enzymatic method. Although the LODs between the proposed methods differed, all of the three methods confirmed BA-forming potential of the three strains. Furthermore, the proposed methods were applied for the analysis of three indigenous strains: MKBT-282 (BA+), MKBT-307 (BA-) and MKBT-325 (BA+). The positive or negative BA-forming potential was confirmed with all of the three proposed methods.
Keywords:lactic acid bacteria, biogenic amines, histamine, grape
Year of publishing:2020
COBISS_ID:22309891 Link is opened in a new window
URN:URN:SI:UNG:REP:6H4G5EOH
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Record is a part of a monograph

Title:The 5th International Conference New Trends on Sensing - Monitoring - Telediagnosis for Life Sciences
Subtitle:NT-SMT-LS 2020
Publisher:Transilvania University Publishing House
ISBN:978-606-19-1266-7
Place of publishing:Brasov
Year of publishing:2020
Editors:Mihaela Badea, Laura Elena Gaman, Laura Floroian, Liviu Gaceu, Patrizia Restani, Jean-Louis Marty, Eleonora-Mihaela Ungureanu

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