| Naslov: | Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo |
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| Avtorji: | ID Montón Silva, Alejandro (Avtor)
ID Lapenta, Fabio (Avtor)
ID Stefan, Alessandra (Avtor)
ID Dal Piaz, Fabrizio (Avtor)
ID Ceccarelli, Alessandro (Avtor)
ID Perrone, Alessandro (Avtor)
ID Hochkoeppler, Alejandro (Avtor) |
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| Datoteke: |
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Delo ni kategorizirano |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | UNG - Univerza v Novi Gorici
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| Opis: | According to current models, dimeric DNA Polymerases coordinate the replication of DNA leading and lagging strands. However, it was recently shown that trimeric DNA Polymerases, assembled in vitro, replicate the lagging strand more efficiently than dimeric replicases. Here we show that the τ, α, ε, and θ subunits of Escherichia coli DNA Polymerase III can be assembled in vivo, yielding the trimeric τ3α3ε3θ3 complex. Further, we propose a molecular model of this complex, whose catalytic action was investigated using model DNA substrates. Our observations indicate that trimeric DNA replicases reduce the gap between leading and lagging strand synthesis. |
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| Ključne besede: | Trimeric DNA Polymerase, E. coli, DNA Polymerase III, τ3α3ε3θ3 complex, Triple DNA extension |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2015 |
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| Št. strani: | 14-20 |
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| Številčenje: | 462, 1 |
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| PID: | 20.500.12556/RUNG-6227 |
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| COBISS.SI-ID: | 40264197 |
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| DOI: | 10.1016/j.bbrc.2015.04.067 |
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| NUK URN: | URN:SI:UNG:REP:GTNYG29F |
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| Datum objave v RUNG: | 27.01.2021 |
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| Število ogledov: | 2625 |
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| Število prenosov: | 0 |
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| Metapodatki: |    |
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| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
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