Repozitorij Univerze v Novi Gorici

Izpis gradiva
A+ | A- | Pomoč | SLO | ENG

Naslov:Postnatal dynamics of Zeb2 expression in rat brain : analysis of novel 3′ UTR sequence reveals a miR-9 interacting site
Avtorji:ID Kropivšek, Klara (Avtor)
ID Pickford, Jasmine (Avtor)
ID Carter, David A. (Avtor)
Datoteke:URL http://link.springer.com/content/pdf/10.1007/s12031-013-0146-x.pdf
 
URL http://link.springer.com/article/10.1007/s12031-013-0146-x/fulltext.html
 
URL http://link.springer.com/content/pdf/10.1007/s12031-013-0146-x
 
Jezik:Angleški jezik
Vrsta gradiva:Neznano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:ZEB2 is a transcription factor with established roles in neurogenesis but no defined function in postnatal brain despite extensive neuronal expression in telencephalic structures. Multiple, incompletely annotated transcripts derive from the Zeb2 locus; the purpose of the present study was to structurally characterize rat brain Zeb2 transcripts with respect to 3′ untranslated (UTR) sequence in order to understand Zeb2 transcript regulation including possible interactions with regulatory molecules such as neuronal miRNAs. We cloned a 5054-nucleotide Zeb2 3′ UTR that is included in the most abundant Zeb2 transcript in neonatal rat brain. Unique features of the distal 3′ UTR region included a number of brain-specific miRNA target sites; a highly conserved miR-9 target site at 3′ UTR position 4097 was selected for functional verification in transfection experiments. Parallel analysis of Zeb2 transcript, ZEB2 protein and miR-9 levels across postnatal brain cortical development revealed a significant accumulation of ZEB2 protein levels only between postnatal days P2 and P5 that was associated with an acute loss of postnatal miR-9 expression in cortex. These studies demonstrate novel features of Zeb2 gene expression in postnatal rat brain and highlight the importance of full transcript annotation for identifying the complement of potential transcript-interacting regulators.
Ključne besede:transcription factor, RNA, untranslated region, miRNA, brain, rat
Datum objave:01.01.2014
Leto izida:2014
Št. strani:str. 138-147
Številčenje:Vol. 52, Vol. 52
PID:20.500.12556/RUNG-9468 Novo okno
ISSN:0895-8696
COBISS.SI-ID:214572035 Novo okno
UDK:57
ISSN pri članku:1559-1166
eISSN:1559-1166
DOI:10.1007/s12031-013-0146-x Novo okno
NUK URN:URN:SI:UNG:REP:DAVIAJUQ
Datum objave v RUNG:11.11.2024
Število ogledov:565
Število prenosov:6
Metapodatki:XML DC-XML DC-RDF
:
KROPIVŠEK, Klara, PICKFORD, Jasmine in CARTER, David A., 2014, Postnatal dynamics of Zeb2 expression in rat brain : analysis of novel 3′ UTR sequence reveals a miR-9 interacting site. Journal of molecular neuroscience [na spletu]. 2014. Vol. 52, no. 52, p. 138–147. [Dostopano 12 april 2025]. DOI 10.1007/s12031-013-0146-x. Pridobljeno s: http://link.springer.com/content/pdf/10.1007/s12031-013-0146-x
Kopiraj citat
  
Skupna ocena:
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
Objavi na:Bookmark and Share


Postavite miškin kazalec na naslov za izpis povzetka. Klik na naslov izpiše podrobnosti ali sproži prenos.

Gradivo je del revije

Naslov:Journal of molecular neuroscience
Skrajšan naslov:J. mol. neurosci.
Založnik:Humana Press
ISSN:1559-1166
COBISS.SI-ID:513695257 Novo okno

Licence

Licenca:Drugo
Opis:http://www.springer.com/tdm
Začetek licenciranja:25.10.2013

Nazaj