20.500.12556/RUNG-3064-55912669-33a0-6520-c8d7-18453b1b60b4
Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
nanobodies
uveal melanoma
patient derived xenografts
MUC18
membrane surface biomarkers
panning
tumor polyclonality
true
true
false
Angleški jezik
Ni določen
Delo ni kategorizirano
2017-04-19 12:54:14
2017-04-19 14:32:56
2023-06-09 03:20:15
0000-00-00 00:00:00
2017
0
0
11
30
2017
0000-00-00
Zaloznikova
NiDoloceno
NiDoloceno
0000-00-00
0000-00-00
0000-00-00
4768763
10.1111/pcmr.12577
URN:SI:UNG:REP:TFTBEVVZ
Univerza v Novi Gorici
0
1
0