21. Instruct-ERIC network : biophysical characterization of antigen-nanobody complexesClaudia D'Ercole, 2024, objavljeni povzetek znanstvenega prispevka na konferenci Opis: Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature which may lead to their massive decline [1]. Understanding the molecular mechanisms of specific stress conditions and monitoring the fluctuations of reliable forest plant biomarkers with affordable methods would be instrumental for assessing stress levels over the time. Ascorbate peroxidase (APX) represents a suitable plant biomarker. APX is a hydrogen peroxide-scavenging enzyme the critical role of which has been described in several plants, both herbaceous and woody. Its activity generally increases under oxidative stress during which its peroxide detoxifying function is part of the wider ascorbate-glutathione cycle [2]. The development of reagents to detect such fluctuations would help the evaluation of plant physiological conditions. In this study, nanobodies (Nbs) targeting APX have been identified. Nbs correspond to the variable domain of heavy chain-only antibodies derived from camelids. They are small (15 kDa), stable, and can be easily produced in bacteria fused to different protein tags according to the downstream applications [3]. After their isolation by biopanning against soluble APX, they have been produced and underwent a biophysical characterization in combination with their antigen (APX-Nb complex) to identify the best binders in terms of stability and affinity. The protein complex characterization was supported by Instruct-ERIC and mainly performed at the BIOCEV institute of Prague. Data from Mass Photometry and Dynamic Light scattering evidenced the formation of the protein complexes, whereas the preliminary data of Hydrogen-Deuterium Exchange Mass Spectrometry, performed with the aim of identifying the residues involved in the paratope/epitope interface, were insufficient to clarify the issue and rather suggested that the interaction has low affinity. This indication was then confirmed by ELISA assay. The combination of multiple methods allowed a comprehensive sample characterization which will require further structural analyses to provide a complete picture of the APX-Nb complex.
[1] G. Marie. B. C. M. B. C. Walters, “Forest decline and tree mortality in a southeastern Ohio oak-hickory forest,” Ohio Journal of Science , vol. 97, 1997.
[2] O. Chew, J. Whelan, and A. H. Millar, “Molecular Definition of the Ascorbate-Glutathione Cycle in Arabidopsis Mitochondria Reveals Dual Targeting of Antioxidant Defenses in Plants,” Journal of Biological Chemistry, vol. 278, no. 47, 2003, doi: 10.1074/jbc.M307525200.
[3] S. Muyldermans, “A guide to: generation and design of nanobodies,” FEBS J, vol. 288, no. 7, pp. 2084–2102, Apr. 2021, doi: 10.1111/febs.15515.
Ključne besede: nanobody, ascorbate peroxidase, plant stress, protein complex, biophysical methodologies
Objavljeno v RUNG: 31.05.2024; Ogledov: 319; Prenosov: 0
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22. Multi-layer palladium diselenide as a contact material for two-dimensional tungsten diselenide field-effect transistorsGennadiy Murastov, Muhammad Awais Aslam, Simon Leitner, Vadym Tkachuk, Iva Plutnarová, Egon Pavlica, Raul D. Rodriguez, Zdeněk Sofer, Aleksandar Matković, 2024, izvirni znanstveni članek Opis: Tungsten diselenide (WSe2) has emerged as a promising ambipolar semiconductor material for field-effect transistors (FETs) due to its unique electronic properties, including a sizeable band gap, high carrier mobility, and remarkable on–off ratio. However, engineering the contacts to WSe2 remains an issue, and high contact barriers prevent the utilization of the full performance in electronic applications. Furthermore, it could be possible to tune the contacts to WSe2 for effective electron or hole injection and consequently pin the threshold voltage to either conduction or valence band. This would be the way to achieve complementary metal–oxide–semiconductor devices without doping of the channel material.This study investigates the behaviour of two-dimensional WSe2 field-effect transistors with multi-layer palladium diselenide (PdSe2) as a contact material. We demonstrate that PdSe2 contacts favour hole injection while preserving the ambipolar nature of the channel material. This consequently yields high-performance p-type WSe2 devices with PdSe2 van der Waals contacts. Further, we explore the tunability of the contact interface by selective laser alteration of the WSe2 under the contacts, enabling pinning of the threshold voltage to the valence band of WSe2, yielding pure p-type operation of the devices.
Ključne besede: field-effect transistor, tungsten diselenide, van der Waals, two-dimensional materials
Objavljeno v RUNG: 29.05.2024; Ogledov: 377; Prenosov: 3
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23. Sustainable digital preservation of new media artNarvika Bovcon, Eszter Polónyi, Jaka Železnikar, Aleš Vaupotič, 2023, objavljeni znanstveni prispevek na konferenci Opis: This paper is about two pilot studies conducted in 2022 that aimed to develop a model for preserving and archiving new media art work in the context of a research project on the sustainable digital preservation of new media art that is being co-hosted by the Museum of Modern Art in Ljubljana. As the works of art selected for the study by Slovenian new media art pioneers Vuk Ćosić (2000) and Srečo Dragan (2005) were technically obsolete or non-functional by the time of the study, the question of how to bring the artworks back into existence and what components of each artwork to include in the collection and preservation process constituted one aspect of our research. But this process of reconstruction also raised questions about how the preservation of media art is reshaping the practice of archiving within an institution whose holdings were, until recently, largely in traditional mediums. An interdisciplinary approach addressed the problem from different points of view, involving the practitioners, experts from art-history, museology, computer science, media theory and intellectual property rights.
Ključne besede: digital cultural heritage, new media art preservation, new media art archives
Objavljeno v RUNG: 28.05.2024; Ogledov: 289; Prenosov: 2
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26. Oxidative potential of particulate matter and its association to respiratory health endpoints in high-altitude cities in BoliviaLucille Borlaza-Lacoste, Valeria Mardoñez, Anouk Marsal, Ian Hough, Thuy Vy Dinh Ngoc, Pamela Dominutti, Jean-Luc Jaffrezo, Andrés Alastuey, Jean-Luc Besombes, Griša Močnik, 2024, izvirni znanstveni članek Ključne besede: particulate matter, oxidative potential, respiratory health, Bolivia, source apportionment, Positive matrix factorization, Poisson regression
Objavljeno v RUNG: 22.05.2024; Ogledov: 323; Prenosov: 0
 Povezava na datoteko
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27. Differential scanning calorimetry of proteins and the two-state model : comparison of two formulasKnarik Yeritsyan, Artem Badasyan, 2024, izvirni znanstveni članek Opis: Differential Scanning Calorimetry (DSC) is a regular and powerful tool to measure the specific heat profile of various materials. In order to connect the measured profile to the properties of a particular protein, a model is required to fit. We discuss here the application of an exact two-state formula with its approximation and process the DSC experimental data on protein folding in water. The approximate formula relies on the smallness of the transition interval, which is different for each protein. With an example of the set of 33 different proteins, we show the practical validity of the approximation and the equivalence of exact and approximate two-state formulas for processing DSC data.
Ključne besede: protein folding, differential scanning calorimetry, heat capacity, two-state model, Hawley model
Objavljeno v RUNG: 21.05.2024; Ogledov: 356; Prenosov: 3
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28. JWST photometric time-delay and magnification measurements for the triply imaged type ia "SN H0pe" at z=1.78Justin Pierel, B. L. Frye, M. Pascale, Gabriel Bartosch Caminha, W. Chen, S. Dhawan, D. Gilman, M. Grayling, Mateusz Bronikowski, Tanja Petrushevska, 2024, izvirni znanstveni članek Ključne besede: supernova, strong lensing, cosmology, Hubble constant, James Webb Space Telescope
Objavljeno v RUNG: 21.05.2024; Ogledov: 376; Prenosov: 3
Celotno besedilo (1,37 MB)
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