21. The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticlesEric Voltà-Durán, Julieta M. Sánchez, Hèctor López-Laguna, Eloi Parladé, Laura Sánchez-García, Alejandro Sánchez-Chardi, Ario De Marco, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, 2022, izvirni znanstveni članek Ključne besede: protein nanoparticles, nanobodies, protein self-assembling Objavljeno v RUNG: 15.02.2022; Ogledov: 1654; Prenosov: 36
Celotno besedilo (5,71 MB) |
22. Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cellsMatteo De March, Michela Terdoslavich, Sulena Polez, Corrado Guarnaccia, Monica Poggianella, Alessandro Marcello, Nataša Skoko, Ario De Marco, 2022, izvirni znanstveni članek Ključne besede: SARS-CoV-2, spike RBD, ExpiCHO, recombinant protein Objavljeno v RUNG: 15.02.2022; Ogledov: 1803; Prenosov: 0 Gradivo ima več datotek! Več... |
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24. Biofabrication of functional protein nanoparticles through simple His-tag engineeringHèctor López-Laguna, Julieta M. Sánchez, José Vicente Carratalá, Mauricio Rojas-Peña, Laura Sánchez-García, Eloi Parladé, Alejandro Sánchez-Chardi, Eric Voltà-Durán, Naroa Serna, Ario De Marco, 2021, izvirni znanstveni članek Ključne besede: nanobodies, protein nanoparticles, controlled aggregation Objavljeno v RUNG: 24.09.2021; Ogledov: 1950; Prenosov: 37
Povezava na celotno besedilo Gradivo ima več datotek! Več... |
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26. Self-assembled nanobodies as selectively targeted, nanostructured, and multivalent materialsLaura Sánchez-García, Eric Voltà-Durán, Eloi Parladé, Elisa Mazzega, Alejandro Sánchez-Chardi, Naroa Serna, Hèctor López-Laguna, Mara Mitstorfer, Ugutz Unzueta, Esther Vázquez, Antonio Villaverde, Ario De Marco, 2021, pregledni znanstveni članek Ključne besede: nanobodies, nanoparticles, fluorescent reagents, protein self-assembling Objavljeno v RUNG: 30.06.2021; Ogledov: 2219; Prenosov: 0 Gradivo ima več datotek! Več... |
27. Quality control of protein reagents for the improvement of research data reproducibilityArio De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, drugi znanstveni članki Ključne besede: protein quality, protein aggregation, data reproducibility Objavljeno v RUNG: 17.05.2021; Ogledov: 2220; Prenosov: 13
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28. Implicit water model within the Zimm-Bragg approach to analyze experimental data for heat and cold denaturation of proteinsArtem Badasyan, Sh. A. Tonoyan, Matjaž Valant, Jože Grdadolnik, 2021, izvirni znanstveni članek Opis: Studies of biopolymer conformations essentially rely on theoretical models that are routinely
used to process and analyze experimental data. While modern experiments allow study of
single molecules in vivo, corresponding theories date back to the early 1950s and require an
essential update to include the recent significant progress in the description of water. The
Hamiltonian formulation of the Zimm-Bragg model we propose includes a simplified, yet
explicit model of water-polypeptide interactions that transforms into the equivalent implicit
description after performing the summation of solvent degrees of freedom in the partition
function. Here we show that our model fits very well to the circular dichroism experimental
data for both heat and cold denaturation and provides the energies of inter- and intra-
molecular H-bonds, unavailable with other processing methods. The revealed delicate
balance between these energies determines the conditions for the existence of cold dena-
turation and thus clarifies its absence in some proteins. Ključne besede: protein folding, cold denaturation, water, Zimm-Bragg model Objavljeno v RUNG: 06.05.2021; Ogledov: 2275; Prenosov: 15
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29. Community-wide experimental evaluation of the PROSS stability-design methodYoav Peleg, Renaud Vincentelli, Brett M. Collins, Kai-En Chen, Emma K. Livingstone, Saroja Weeratunga, Natalya Leneva, Qian Guo, Kim Remans, Ario De Marco, 2021, izvirni znanstveni članek Ključne besede: protein stability, recombinant production, optimization algorithm, PROSS Objavljeno v RUNG: 23.04.2021; Ogledov: 2395; Prenosov: 0 Gradivo ima več datotek! Več... |
30. Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale surveyNick Berrow, Ario De Marco, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, izvirni znanstveni članek Ključne besede: protein quality, biophysical analysis, protein aggregates Objavljeno v RUNG: 22.04.2021; Ogledov: 2233; Prenosov: 0 Gradivo ima več datotek! Več... |