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131.
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Sandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, original scientific article

Abstract: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Keywords: nanobodies, synthetic phage display library, in vitro panning
Published in RUNG: 17.08.2016; Views: 6584; Downloads: 247
.pdf Full text (4,32 MB)

132.
Selection criteria and engineering of nanobodies for oncological applications
Ario De Marco, invited lecture at foreign university

Abstract: Lecture relative to the biotechnological features of nanobodies
Keywords: nanobodies, phage display, antibody engineering, panning
Published in RUNG: 14.07.2016; Views: 5227; Downloads: 0
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133.
Co-expression and co-purification of antigen-antibody complexes in bacterial cytoplasm and periplasm
Ario De Marco, 2014, independent scientific component part or a chapter in a monograph

Keywords: rekombinantni proteini, protitelesa, imunoafinitetno čiščenje, kristalizacija proteinov
Published in RUNG: 16.06.2016; Views: 5589; Downloads: 18
URL Link to full text

134.
An intrabody specific for the nucleophosmin carboxy-terminal mutant and fused to a nuclear localization sequence binds its antigen but fails to relocate it in the nucleus
Ario De Marco, Chiara Martinelli, Emanuela Colombo, Daniele Piccini, Cristina Sironi, Pier Giuseppe Pelicci, 2014, original scientific article

Keywords: intrabody, leukemia, protein shuttling, recombinant fusion protein, single-chain antibody
Published in RUNG: 16.06.2016; Views: 4991; Downloads: 25
URL Link to full text

135.
Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria
Ario De Marco, 2012, other scientific articles

Keywords: recombinant protein expression, disulfide, bonds, redox conditions
Published in RUNG: 16.06.2016; Views: 5063; Downloads: 137
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136.
The Trip Adviser guide to the protein science world : a proposal to improve the awareness concerning the quality of recombinant proteins
Ario De Marco, Mario Lebendiker, Tsafi Danieli, 2014, other scientific articles

Keywords: protein quality, protein production, biophysics
Published in RUNG: 16.06.2016; Views: 5706; Downloads: 144
URL Link to full text

137.
Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies
Ario De Marco, Selma Djender, Anne Beugnet, Aurelie Schneider, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, 2014, original scientific article

Keywords: recombinant antibodies, cytoplasmic expression, functional tags
Published in RUNG: 16.06.2016; Views: 5236; Downloads: 134
URL Link to full text

138.
Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs
Ario De Marco, 2015, review article

Keywords: recombinant antibody, nanobodies, antibody display, neutralising antibodies
Published in RUNG: 17.05.2016; Views: 6064; Downloads: 200
URL Link to full text

139.
Evaluation of a novel human IgG1 anti-claudin3 antibody that specifically recognizes its aberrantly localized antigen in ovarian cancer cells and that is suitable for selective drug delivery
Ario De Marco, Chiara Romani, 2015, original scientific article

Keywords: therapeutic targets, claudins, tight junctions, human antibodies
Published in RUNG: 17.05.2016; Views: 5130; Downloads: 142
URL Link to full text

140.
Computational design of customised nanobodies for biotechnological applications
Miguel Soler, Ario De Marco, Sara Fortuna, 2016, unpublished conference contribution

Abstract: In silico modeling to improve the biophysical characteristics of recombinant single-domain antibodies
Keywords: nanobodies, modeling, protein stability, antibody humanization, molecular dynamics
Published in RUNG: 26.04.2016; Views: 5697; Downloads: 0
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