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How to react to the necessity of a sustainable animal production? The EcoLamb project.
Petra Makorič, Ario de Marco, Martina Bergant, Tanja Peric, 2019, published scientific conference contribution

Abstract: EcoLamb assesses the sustainability of diverse European sheep production systems focusing on the ecological footprint, animal welfare aspects and nutrition value of lamb meat. The outcomes of these assessments will be used to understand the potential future barriers that limit the innovative capacity and development of the sector and the opportunities that may provide a future market niche against competitive products from other global markets. Farm solutions that incorporate consumer expectations for animal welfare and meat quality will enhance the competitiveness of Europe's lamb meat sector. The project engages trans-national research and industry stakeholders from 6 countries made up of Germany, Italy, Portugal, Slovenia, Spain and Turkey to analyse on 20 case study farms resource-efficient, competitive and low-carbon lamb production models. Direct linkage between animal welfare, meat quality and pharmaceutical use will also be determined using innovative Precision Farming techniques. The project will produce a toolbox of recommendations for productive sheep farm management, supply chain and marketing on how to improve the acceptability of lamb meat by consumers. Thus, the multidisciplinary approach and the multi-actor involvement of the EU sheep sector will assist in re-designing critical aspects to increase society acceptance and the place of lamb meat in future diets and the outcomes of the project will be used by stakeholders to promote changes in farm management, marketing and processing of meat from sheep. Additionally, results will be used by farm consultants, farmer groups and policy officers to re-design consulting approaches and plan new initiatives to make all aspects of the European sheep industry more sustainable.
Found in: osebi
Keywords: Sustainability, animal production, lamb, welfare, quality, meat, EcoLamb
Published: 26.11.2019; Views: 1048; Downloads: 0
.pdf Fulltext (7,65 MB)

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Nanobodies isolated from pre-immune libraries as tunable biotechnological tools
Ario de Marco, invited lecture at foreign university

Found in: osebi
Keywords: nanobodies, panning, modeling
Published: 27.02.2020; Views: 728; Downloads: 0
.pdf Fulltext (61,23 KB)

35.
Recombinant expression of nanobodies and nanobody-derived immunoreagents
Ario de Marco, 2020, review article

Found in: osebi
Keywords: nanobodies, prokaryotic expression, eukaryotic expression, inclusion bodies
Published: 14.04.2020; Views: 701; Downloads: 0
.pdf Fulltext (739,02 KB)

36.
FUNCTIONALIZED NANOBODIES AS DIAGNOSTIC REAGENTS FOR THE DETECTION OF TOXIC MICROALGAE
Sandra Oloketuyi, 2020, doctoral dissertation

Abstract: Early detection and monitoring of toxic microalgae remains a challenge that will be necessary to overcome for understanding harmful algal bloom events and their consequent ecological and environmental impacts. Although there are diagnostic techniques based on microscopy, fluorescence in situ hybridization (FISH), DNA microarrays, real-time PCR, sandwich hybridization assays employed for the detection of harmful algal species, such techniques are not suitable for field monitoring and especially for the quantification of Alexandrium minutum due to abundance of cryptic species that cannot be differentiated based on morphology or genomic traits. These approaches are also time-consuming and laborious, hence there is need for alternative simple, rapid and cost-effective methods. In this study, our aim was to develop different nanobody-based capture strategies for the detection of A. minutum. Nanobodies were successfully displayed on the surface of Escherichia coli to facilitate detection of A. minutum. The method is simple and cost effective as no antibody purification step is necessary. As an alternative, A. minutum was quantified by exploiting the peroxidase activity of a G-quadruplex DNAzyme covalently bound to nanobodies and by means of a label free electrochemical immunosensor which exploit nanobodies bound via an irreversible SpyTag-SpyCatcher system as the specific immunocapture element. Such immunosensor was prepared by immobilizing the SpyTagged anti-A. minutum nanobody (SpyTagged C1) by means of a selfassembled monolayer (SAMs) of L- cysteine (L-Cys) displayed onto a gold nanoparticle-modified glassy carbon electrode. Electrochemical Impedance Spectroscopy (EIS) was performed to measure the electrical response of the circuit as the function of A. minutum immunocaptured cells. The results of EIS studies confirmed that the sensing chip detected A. minutum selectively, exhibited a wide dynamic range spanning from 103 to 109 cell L-1 and a limit of detection of 3.1× 103 cell L-1. The immunosensor data can be recorded and then analyzed with a portable potentiostat. Such device can be interfaced with a smart phone, a condition suitable for the rapid A. minutum quantification in situ.
Found in: osebi
Keywords: Alexandrium minutum, nanobodies, bioreagents, immunosensor, toxic microalgae
Published: 17.06.2020; Views: 1209; Downloads: 54
.pdf Fulltext (2,95 MB)

37.
Recombinant proteins co-expressed and co-purified in the presence of antibody fragments
Ario de Marco, 2021, independent scientific component part or a chapter in a monograph

Found in: osebi
Keywords: protein complexes, affinity purification, nanobodies
Published: 17.12.2020; Views: 422; Downloads: 0
.pdf Fulltext (94,45 KB)
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