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Co-expression and co-purification of antigen-antibody complexes in bacterial cytoplasm and periplasm
Ario De Marco, 2014, independent scientific component part or a chapter in a monograph

Found in: osebi
Keywords: rekombinantni proteini, protitelesa, imunoafinitetno čiščenje, kristalizacija proteinov
Published: 16.06.2016; Views: 2314; Downloads: 6
URL Fulltext (0,00 KB)

The Trip Adviser guide to the protein science world
Ario De Marco, Mario Lebendiker, Tsafi Danieli, 2014, short scientific article

Found in: osebi
Keywords: protein quality, protein production, biophysics
Published: 16.06.2016; Views: 2221; Downloads: 91
URL Fulltext (0,00 KB)

Is there an approach for optimizing the specific binding of antibodies to target cells?
Ario De Marco, invited lecture at foreign university

Found in: osebi
Keywords: Nanobodies, avidity, modeling, off-target accumulation
Published: 31.01.2017; Views: 2202; Downloads: 0
.pdf Fulltext (81,48 KB)

Nanobody technology: principles & applications
Ario De Marco, invited lecture at foreign university

Abstract: Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation
Found in: osebi
Keywords: Nanobodies, in vitro panning, cancer biomarkers, recombinant antibody engineering
Published: 03.05.2017; Views: 2789; Downloads: 0
.pdf Fulltext (37,92 KB)

Biotechnological applications of nanobody pre-immune libraries
Ario De Marco, invited lecture at foreign university

Found in: osebi
Keywords: nanobodies, pre-immune libraries, in vitro selection, biomarkers, recombinant proteins
Published: 16.05.2017; Views: 1895; Downloads: 0
.pdf Fulltext (331,92 KB)

Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, original scientific article

Abstract: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Found in: osebi
Keywords: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Published: 19.10.2017; Views: 1888; Downloads: 0
.pdf Fulltext (982,69 KB)

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