1. Silica-coated Bi2Se3 topological insulator nanoparticles: preserving their optical properties and make them biocompatibleBlaž Belec, Nina Kostevšek, Giullia Della Pelle, Sebastjan Nemec, Slavko Kralj, Martina Bergant Marušič, Sandra Gardonio, Mattia Fanetti, Matjaž Valant, 2023, published scientific conference contribution abstract Keywords: topological insulator, bismuth selenide, photo-thermal material, biocompatibility, nanoparticles
Published in RUNG: 19.10.2023; Views: 324; Downloads: 3
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2. Contribution of APOBEC proteins 3A and 3B to the oncogenicity of HPV virusesNika Marija Lovšin, Fabio Lapenta, Urša Pečar Fonović, Katerina Jazbec, Elvira Maličev, Martina Bergant Marušič, 2023, published scientific conference contribution abstract Keywords: APOBEC, HPV, oncogenicity, gene expression
Published in RUNG: 10.10.2023; Views: 259; Downloads: 9
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3. In vitro tumor hypoxia imaging with fluorescent covalent organic frameworksTina Skorjanc, Dinesh Shetty, Damjan Makuc, Gregor Mali, Martina Bergant Marušič, Matjaz Valant, 2023, published scientific conference contribution abstract Abstract: Hypoxia refers to a condition where cells and tissues experience low, inadequate levels of O2. While healthy tissues are typically supplied with sufficient O2 (normoxia), cancerous tissues commonly face hypoxia due to the tumor’s extraordinarily high demand for oxygen. Various fluorescent small-molecule probes have been designed for selective detection of hypoxia in living cells, but few nanomaterials have been investigated for this type of bioimaging. Herein, we prepare a fluorescent covalent organic framework (COF) with β-ketoenamine linkages and post-synthetically modify it to conjugate hypoxia-sensitive nitroimidazole moieties
into its pores (NI-COF). Stacks of sheets in NI-COF observed under electron microscopy were exfoliated by ultrasonication, and dynamic light scattering measurements confirmed particle size of less than 200 nm. Thus-prepared material exhibited good stability in physiological conditions and low cytotoxicity in in vitro experiments. NI-COF also showed useful fluorescence properties with an emission peak at 490 nm (λex = 420 nm) at both neutral and mildly acidic pH levels that are characteristic of tumor tissues. Encouraged by the favorable properties of the material, we incubated HeLa cells pre-treated in either hypoxic or normoxic conditions with NI-COF. Fluorescence microscopy images demonstrated that the material was preferentially taken up by hypoxic cells, which showed higher fluorescence signal in their interior than cells cultured under normoxia conditions. It is anticipated that this study will stimulate further developments of COFs for imaging various biological conditions.
Keywords: hypoxia, fluorescence, covalent organic frameworks, imaging, tumor cells
Published in RUNG: 19.09.2023; Views: 348; Downloads: 3
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5. Nitroreductase-sensitive fluorescent covalent organic framework for tumor hypoxia imaging in cellsTina Skorjanc, Dinesh Shetty, Sushil Kumar, Damjan Makuc, Gregor Mali, Janez Vovavšek, Martina Bergant Marušič, Matjaz Valant, 2023, original scientific article Keywords: covalent organic frameworks, imaging, hypoxia, fluorescence, cancer cells
Published in RUNG: 17.05.2023; Views: 662; Downloads: 6
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6. Silica Coated Bi2Se3 Topological Insulator Nanoparticles: An Alternative Route to Retain Their Optical Properties and Make Them BiocompatibleBlaž Belec, Nina Kostevšek, Giullia Della Pelle, Sebastijan Nemec, Slavko Kralj, Martina Bergant Marušič, Sandra Gardonio, Mattia Fanttti, Matjaž Valant, 2023, original scientific article Keywords: topological insulator, bismuth selenide, photo-thermal material, biocompatibility, nanoparticles
Published in RUNG: 22.02.2023; Views: 979; Downloads: 0
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8. Diagnostics and Monitoring of COVID-19 Infection – Current UnderstandingMartina Bergant, Ario de Marco, 2020, review, book review, critique Keywords: SARS-CoV-2, RT-PCR, seroconversion, serum biomarkers, neutralizing antibodies
Published in RUNG: 30.09.2022; Views: 824; Downloads: 18
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