21. Regulation and controlling the motility properties of Pseudomonas aeruginosaFazlurrahman Khan, Dung Thuy Nguyen Pham, Sandra Oloketuyi, Young-Mog Kim, 2020, review article Abstract: Chronic infections caused by Pseudomonas aeruginosa have been a major concern as their spread and mortality continue to be on the rise. These infections are majorly attributed to biofilm formation via sequential steps where motility plays an essential role in initial attachment of bacterial cells onto biotic and abiotic surfaces, thereby contributing to multi-drug resistance among pathogens. Therefore, attenuating motility properties can be considered as highly potential for controlling P. aeruginosa biofilm formation. This strategy has employed the use of various natural and chemically synthesized compounds. The present review article explained the importance and regulation of different types of motilities properties. Furthermore, it also covered several important alternative approaches using anti-motility agents which could be helpful for controlling P. aeruginosa biofilm-associated infections. Further studies are required for in-depth understandings about the mechanisms of motilities controlling of these molecules at molecular levels.
Keywords: Biofilm, Motility, Attenuation, Antibiofilm drugs, Pseudomonas aeruginosa
Published in RUNG: 12.01.2021; Views: 2611; Downloads: 0
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22. FUNCTIONALIZED NANOBODIES AS DIAGNOSTIC REAGENTS FOR THE DETECTION OF TOXIC MICROALGAESandra Oloketuyi, 2020, doctoral dissertation Abstract: Early detection and monitoring of toxic microalgae remains a challenge that will be necessary to overcome for understanding harmful algal bloom events and their consequent ecological and environmental impacts. Although there are diagnostic techniques based on microscopy, fluorescence in situ hybridization (FISH), DNA microarrays, real-time PCR, sandwich hybridization assays employed for the detection of harmful algal species, such techniques are not suitable for field monitoring and especially for the quantification of Alexandrium minutum due to abundance of cryptic species that cannot be differentiated based on morphology or genomic traits. These approaches are also time-consuming and laborious, hence there is need for alternative simple, rapid and cost-effective methods. In this study, our aim was to develop different nanobody-based capture strategies for the detection of A. minutum. Nanobodies were successfully displayed on the surface of Escherichia coli to facilitate detection of A. minutum. The method is simple and cost effective as no antibody purification step is necessary. As an alternative, A. minutum was quantified by exploiting the peroxidase activity of a G-quadruplex DNAzyme covalently bound to nanobodies and by means of a label free electrochemical immunosensor which exploit nanobodies bound via an irreversible SpyTag-SpyCatcher system as the specific immunocapture element. Such immunosensor was prepared by immobilizing the SpyTagged anti-A. minutum nanobody (SpyTagged C1) by means of a selfassembled monolayer (SAMs) of L- cysteine (L-Cys) displayed onto a gold nanoparticle-modified glassy carbon electrode. Electrochemical Impedance Spectroscopy (EIS) was performed to measure the electrical response of the circuit as the function of A. minutum immunocaptured cells. The results of EIS studies confirmed that the sensing chip detected A. minutum selectively, exhibited a wide dynamic range spanning from 103 to 109 cell L-1 and a limit of detection of 3.1× 103 cell L-1. The immunosensor data can be recorded and then analyzed with a portable potentiostat. Such device can be interfaced with a smart phone, a condition suitable for the rapid A. minutum quantification in situ.
Keywords: Alexandrium minutum, nanobodies, bioreagents, immunosensor, toxic microalgae
Published in RUNG: 17.06.2020; Views: 4022; Downloads: 231
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24. Electrochemical immunosensor functionalized with nanobodies for the detection of the toxic microalgae Alexandrium minutum using glassy carbon electrode modified with gold nanoparticlesSandra Oloketuyi, Elisa Mazzega, Janez Zavašnik, Kingkan Pungjunun, Kurt Kalcher, Ario De Marco, Eda Mehmeti, 2020, original scientific article Keywords: biosensors, toxic microalgae, nanobodies, impedance
Published in RUNG: 07.02.2020; Views: 3214; Downloads: 0
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25. Bacterial surface display of nanobodies against cancer and toxic micoalgal cellsSandra Oloketuyi, Carina Dilkaute, Elisa Mazzega, Joachim Jose, Ario De Marco, 2019, published scientific conference contribution abstract Keywords: bacteria surface display, nanobodies, HER2, A. minutum
Published in RUNG: 18.11.2019; Views: 3332; Downloads: 99
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26. Functionalized nanobodies: A bio-recognition molecule for the detection of the toxic microalgae Alexandrium minutum by means of an electrochemical immunosensorSandra Oloketuyi, Elisa Mazzega, Eda Mehmeti, Kurt Kalcher, Ario De Marco, 2019, published scientific conference contribution abstract Keywords: Biosensors, nanobodies, impedance, immunocapture, SpyTag
Published in RUNG: 18.11.2019; Views: 4091; Downloads: 102
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27. Quantitative detection of Microcystis aeruginosa in fresh water using single domain antibodies (VHHs)Gbenga Folorunsho Oginni, Sandra Oloketuyi, Elisa Mazzega, Mladen Franko, Hanna Budasheva, Dorota Korte, Ario De Marco, published scientific conference contribution abstract Keywords: thermal lens spectrometry, nanobodies, fluobodies, cyanobacteria
Published in RUNG: 18.11.2019; Views: 3581; Downloads: 122
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28. A compact nanobody-DNAzyme conjugate enables antigen detection and signal amplificationGiulio Bernardinelli, Sandra Oloketuyi, Franziska Werner, Elisa Mazzega, Bjorn Hogberg, Ario De Marco, 2020, original scientific article Keywords: DNAzyme, nanobodies, diagnostics, A. minutum
Published in RUNG: 10.11.2019; Views: 3297; Downloads: 0
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29. Nanobody Engineering Provides Diagnostic Reagents For The Quantification Of The Toxic Microalgae Alexandrium minutumRobert Alvin Bernedo-Navarro, Sandra Oloketuyi, Giulio Bernardinelli, Eda Mehmeti, Kurt Kalcher, Ario De Marco, 2019, published scientific conference contribution abstract Keywords: Nanobodies, DNAzyme, electrochemical impedance spectroscopy, toxic microalgae
Published in RUNG: 09.09.2019; Views: 3510; Downloads: 0
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