MOLECULAR MECHANISMS REGULATING ATP SIGNALING IN MOUSE SENSORY NEURONSTanja Bele
, 2015, doctoral dissertation
Abstract: Coordinated and harmonized neuronal and glial responses to variations in extracellular levels of active soluble mediators such as ATP are essential in controlling neuronal activity. In pathological conditions involving sensory nervous system, elevations in extracellular ATP levels are believed to be one of the main reason for neuronal sensitization.
This notion led us to explore mechanisms of ATP release in sensory ganglia and we found that association among P2X purinergic receptors, their downstream effectors (CASK and CaMKII) and hemichannel Panx1 regulates inhibition of ATP release in basal conditions and that same players are involved in P2X3 receptor evoked-ATP release which globally suggest that even if observed proteins are expressed in different cells, they could be modulated by similar mechanisms and are possibly part of an “ATP-keeper molecular system” that finely regulates extracellular levels of ATP by its sensing and further adjustments of peculiar extracellular concentrations.
Further we showed that P2X3 receptors interact with Panx1 in sensory neurons and that molecular coupling between P2X3, CASK and Panx1 contributes to decoding of the complex purinergic signaling involved in nociception which represents a novel and interesting mechanism of pain regulation that could be precisely targeted in order to alleviate tedious disorders of sensory neurons.
Found in: ključnih besedah
Summary of found: ...led us to explore mechanisms of ATP release in sensory ganglia and we found that...
Keywords: ATP, ATP release, purinergic signaling, trigeminal ganglion, pain, migraine, P2X3, CASK, Pannexin-1, synaptic strength
Published: 10.12.2015; Views: 3732; Downloads: 182
Fulltext (3,75 MB)
Reducing treatments in cattle superovulation protocols by combining a pituitary extract with a 5% hyaluronan solution: Is it able to diminish activation of the hypothalamic pituitary adrenal axis compared to the traditional protocol?Tanja Peric
, Maurizio Monaci
, Lakamy Sylla
, Antonella Comin
, Tatiana Sbaragli
, Andrea Biancucci
, Giuseppe Stradaioli
, 2016, original scientific article
Abstract: Traditional superovulation protocols that include multiple gonadotropin treatments are time-consuming and labor intensive. These protocols require multiple handling and restraining of embryo donors. This will likely increase the risks of injuries in both animals and humans and induce stress that may lead to a reduced superovulatory response. These are more evident when working with cattle that are rarely handled or raised on extensive grazing. The objectives of this experiment were to compare the efficacy of a split-injection protocol of porcine pituitary-derived porcine FSH (pFSH) preparation (slow release [SR] group) to the traditional 4-day treatment with pFSH administered twice daily (C group) and to determine the concentrations of cortisol in the hair as a marker of activation of the hypothalamic-pituitary-adrenal (HPA) axis during the two superovulatory treatments. Thirty-two heifers were stimulated twice in a 2 × 2 crossover design and compared for ovarian response and numbers and characteristics of recovered ova-embryo among treatments. No differences between SR and C groups were found in terms of percentage of responsive animals (100% vs. 93.8%) and ovulation rate (83.7 ± 1.1 vs. 79.5 ± 1.0%). A positive correlation was found between the number of follicles responsive to pFSH (2-8 mm) at the beginning of treatments and the superovulatory response, and no differences were found in these follicular populations between the two treatment groups. The numbers of CLs, ova-embryos, fertilized ova, transferable and freezable embryos recovered per cow were found to be significantly higher in SR compared with C group (14.0 ± 1.6 vs. 10.6 ± 1.0, 12.1 ± 1.6 vs. 7.6 ± 1.0, 11.1 ± 1.1 vs. 7.3 ± 1.0, 9.6 ± 1.4 vs. 6.6 ± 1.0, and 9.4 ± 1.4 vs. 6.0 ± 1.0 for SR and C group, respectively). The SR group produced also a significantly greater number of excellent- and/or good-quality embryos compared with the C group. The concentrations of cortisol in the hair at Days 14 and 21 were significantly greater in the C compared with the SR group (P < 0.05). In conclusion, these results indicate that the dilution of gonadotropin in a 5% hyaluronan solution, reducing the administration frequency, improves the quantitative and qualitative superovulatory response of Marchigiana heifers. Further studies using other breeds of cattle are needed to verify the results herein obtained and to confirm the lower activation of the hypothalamic-pituitary-adrenal axis caused in the donor by the split-injection protocol.
Found in: ključnih besedah
Summary of found: ...porcine pituitary-derived porcine FSH (pFSH) preparation (slow release [SR] group) to the traditional 4-day treatment...
Keywords: Beef heifer, Bovine, Hair cortisol, Hyaluronic acid, Slow release, Superovulation
Published: 01.03.2016; Views: 2841; Downloads: 241
Fulltext (403,15 KB)
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Design of organic macrocycle-modified iron oxide nanoparticles for drug deliveryAli Trabolsi
, John-Carl Olsen
, Farah Benyettou
, Tina Škorjanc
, review article
Found in: ključnih besedah
Summary of found: ...binding modes, drug delivery, drug release, iron oxide nanoparticles, organic macrocycles, supramolecular interactions...
Keywords: binding modes, drug delivery, drug release, iron oxide nanoparticles, organic macrocycles, supramolecular interactions
Published: 02.09.2020; Views: 901; Downloads: 0
Fulltext (3,39 MB)