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1.
Native agarose gels and contact blotting as means to optimize the protocols for the formation of antigen-ligand complexes
Claudia D'Ercole, Ario De Marco, 2024, published scientific conference contribution abstract

Abstract: Background. Protein complexes provide valuable biological information but can be difficult to handle [1]. Therefore, technical advancements designed to improve their manipulation are al-ways useful. Methods. We investigated the opportunity to exploit native agarose gels and the contact bot method for the transfer of native proteins to membranes as means for optimizing the conditions for obtaining stable complexes [2]. As a simple model of protein-protein interactions, an anti-gen-ligand complex was used in which both proteins were fused to reporters. Results. At each step, it was possible to visualize both the antigen, fused to a fluorescent pro-tein, and the ligand, fused to a monomeric ascorbate peroxidase (APEX) and, as such, a way to tune the protocol. The conditions for the complex formation were adapted by modifying the buffer conditions, the concentration of the proteins and of the cross-linkers. Conclusions. The procedure is rapid, inexpensive, and the several detection opportunities al-lows for both the monitoring of complex stability and the preservation of the functionality of its components, which is critical for understanding their biomedical implications and supporting drug discovery. The overall protocol represents a handy alternative to gel filtration, uses very standard and ubiquitous equipment, and can be implemented rapidly and without specific train-ing. References: [1] O. Puig, F. Caspary, G. Rigaut, B. Rutz, E. Bouveret, E. Bragado-Nilsson, M. Wilm, B. Séraphin. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods. (San Diego, Calif.), 24(3), 218–229 [2] C. Sakuma, M. Nakagawa, Y. Tomioka, T. Maruyama, K. Entzminger, J.K. Fleming, T. Shibata, Y. Kurosawa, C.J. Okumura, T. Arakawa, T. Akuta. Western blotting of native proteins from agarose gels. Biotechniques. 2022 May;72(5):207-218
Keywords: protein complexes, contact blotting, native agarose gels, protein interaction
Published in RUNG: 17.06.2024; Views: 342; Downloads: 0
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2.
Morphogenesis of the Postojna Basin karst periphery : dissertation
Astrid Švara, 2023, doctoral dissertation

Abstract: This thesis represents a karstological study on the drainage of the Postojna Basin. It focuses on a multidisciplinary approach, based on fieldwork, computer analyses and dating. The northern study area has the highest: doline density of 108 dolines/km2, number of contact karst features (i.e. 9), and number of collapse dolines (i.e. 19). It has three catchments. The Lokva cuts into the flysch ridges and sinks in the Predjama Cave System at the lowest point of the basin (i.e. 474 m a.s.l.). Between 37 selected caves, 14 were longer than 150 m. The Predjama Cave System was developed in 3 levels and the Postojna Cave System was developed in 2 levels. The vertical passages connecting them are deepest in the Predjama cave, where the vadose zone is up to 250 m deep. By the favourable ponor steepheads and 140 m deep vadose passages, the Hrušica Plateau shows a major uplift phase. The Postojna Cave System, Risovec blind valley and 17 collapse dolines indicate significant past hydrological changes. First, Nanoščica formed the Risovec blind valley, followed by Otoška jama, Tartarus, Male jame, and Artificial tunnel toward E/NE, forming the Vodni dol. At the same time, the ponor of Pivka shifted in the Postojnska jama (at present “Stara jama”), flowing towards N, forming Jeršanove doline. The successive subsidence of the erosional base and the Postojna karst uplift redirected the Nanoščica in Pivka. Now they sink into active parts of the Postojna cave at 511 m a.s.l. The eastern study area has the Unroofed cave Kriva dolina as a former ponor of Pivka. At present the area has springs and favourable (sub)vertical passages. The southern study area has the highest variety of contact karst features (i.e. 5). The Prestranški ravnik represents an aquifer with ponors and springs. It is separated from the Slavinski ravnik, by a flysch belt. In the southern study area, 10 caves were longer than 150 m among 49 selected caves. The Loza Cave System, a case study in Slavinski ravnik, is developed in 3 epiphreatic levels that followed two phases of tectonic uplift with a successive formation of diversion routes through 30-40 m deep vadose passages. The oldest upper cave level has sediments from at least the Gilbert Chron with CW rotations. U-Th dates from speleothems prove vadose speleogenesis before the cave ceiling denudation and collapse from 210 ka to 550 ka (≤1.2 Ma). The middle cave level has epiphreatic sediments at least in the Gauss Chron (i.e. 2.59-3.59 Ma) and reveals 35-38° CCW rotations. The allogenic sediments in the lower cave level show Brunhes and Matuyama Chrons (i.e. <0.78-2.58 Ma). We generally discussed ponor steepheads that develop on steepest slopes on the thrust/fault contact, while blind valleys and border depressions develop on normal stratigraphic contact with mildest slopes. The speleogenesis was mainly driven by relatively quick tectonic uplift, followed by vadose speleogenesis, with intermediate speleogenesis in the epiphreatic zone. Speleogenesis and contact karst features follow the subsidence of the water table, evidenced by cave levels, and active and relict features. Allogenic sediments are followed by speleothems as shift of caves between hydrological zones. The sequence of events repeated 2-3 times. The main local source of allogenic sediments is in the Postojna Basin, represented by the erosion of flysch rocks and alluvium. The mineralogical composition between catchments is similar with no significant change. The regional compressional-tectonic regime has significantly influenced the changes in the drainage of the Postojna Basin during the last 7 Ma, with different uplifts and drop of the karst water table. The major uplift was reflected by the change in the Nanoščica course from the Slavinski ravnik to the Postojna karst from S to N, presumably between 3.59 Ma and >1.77 Ma and represented the last important general shift in the drainage of the Postojna Basin.
Keywords: karst, contact karst, regional tectonic uplift, cave levels, shift from epiphreatic to vadose speleogenesis, Loza Cave System, Postojna drainage basin
Published in RUNG: 05.12.2023; Views: 1097; Downloads: 63
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3.
Technological and analytical review of contact tracing apps for COVID-19 management
Rajan Gupta, Gaurav Pandey, Poonam Chaudhary, Saibal K. Pal, 2021, original scientific article

Abstract: Role of technology is improving for COVID-19 management all around the world. Usage of mobile applications, web applications, cloud computing, and related technologies have helped many public administrators worldwide manage the current pandemic. Contact tracing applications are such mobile app solutions that are used by more than 100 countries today. This study presents a structured research review-based framework related to multiple contact tracing applications. The various components of the framework are related to technological working, design architecture, and feature analysis of the applications, along with the analysis of the acceptance of such applications worldwide. Also, components focusing on the security features and analysis of these applications based on Data Privacy, Security Vetting, and different attacks have been included in the research framework. Many applications are yet to explore the analytical capabilities of the data generated through contact tracing. The various use-cases identified for these applications are detecting positive case probability, identifying a containment zone in the country, finding regional hotspots, monitoring public events & gatherings, identifying sensitive routes, and allocating resources in various regions during the pandemic. This study will act as a guide for the users researching contact tracings applications using the proposed four-layered framework for their app assessment.
Keywords: novel corona virus, location technology, contact tracing applications, Aarogya Setu App, data science, data analysis
Published in RUNG: 02.04.2021; Views: 2243; Downloads: 0
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4.
Technology applications for health safety decision making under COVID-19 pandemic management
Rajan Gupta, Saibal K. Pal, Aayush Khattar, King Baliyan, 2020, published scientific conference contribution

Abstract: A lot of new technological applications are emerging to combat the deadly novel coronavirus. This research study gives an overview of different applications that are developed by Government Institutions, Private Firms, and Individual Citizens across the world. The applications are reviewed based on their widespread use, effectiveness, availability to the broader audience, cost to implement it, concerns regarding the privacy and information collected by these apps and systems. The major eight areas of technology applications covered in this study are Contact Tracing, Social Distancing & Mask Detection, Live-feeds based Dashboards, Information Searching, Big Data and Robotics, Web-based Disease Surveillance Tools, Patient-level Information, Doctor-Patient interaction, and Informatory Chatbots. More than 100 apps were collected for this research survey to conclude the different categories in which technology is being used for decision making. This study will be useful for various health administrators, professionals, researchers, and academicians.
Keywords: COVID-19, coronavirus, technology applications, contact tracing, social distancing, chatbots, data lakes
Published in RUNG: 02.04.2021; Views: 2581; Downloads: 77
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5.
Analysis of COVID-19 tracking tool in india: case study of aarogya setu mobile application : Case Study of Aarogya Setu Mobile Application
Rajan Gupta, Manan Bedi, Prashi Goyal, Srishti Wadhera, Vaishnavi Verma, 2020, original scientific article

Abstract: COVID-19 tracking tools or contact-tracing apps are getting developed at a rapid pace by different governments in their respective countries. This study explores one such tool called Aarogya Setu, developed by the Government of India. It is a mobile application developed under the Health Ministry, as a part of the E-Governance initiative, to track and sensitize the citizens of India in a joint battle against COVID-19 spread. The study aims to understand various useful features of this tool and to present different concepts of data science applied within the application along with its importance in managing the ongoing pandemic. The App uses Bluetooth and GPS technologies to alert a user when they are nearby a COVID-19 infected person. The application uses various Data Science concepts such as Classification, Association Rule Mining, and Clustering to analyze COVID-19 spread in India. The study also shows potential upgradations in the application, which includes usage of Artificial Intelligence and Computer Vision to detect COVID-19 patients. The study would be useful for mobile technology professionals, data science professionals, medical practitioners, health-related frontline workers, public administrators, and government officials.
Keywords: COVID-19, India, contact-tracing, tracking tool, Bluetooth, GPS, COVID19 reporting tool, Aarogya Setu
Published in RUNG: 01.04.2021; Views: 2283; Downloads: 60
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6.
Augmented Reality and Chronotopicality : Literary Itineraries in Gorizia, Trieste and Duino
Narvika Bovcon, Aleš Vaupotič, unpublished conference contribution

Abstract: The reality-virtuality continuum (Paul Milgram et al., 1994) establishes a foundational mixed reality as a spectrum of intermediate options. The ICT based databases are increasingly superimposed on and integrated in our life-world. The new media art – and contemporary media in general – can be seen through two points of view: the technical aspect where the reality is supplemented by (3-D, networked) computerbased data. Secondly, the augmented reality can be construed as a real superimposition of different realities, that is foregrounded using Mikhail Bakhtin’s notion of chronotopicality. The MAST symposium (18. & 19.11.2019, ex-Daimond (xD), Nova Gorica, Slovenia), Pixxelpoint 2019.
Keywords: augmented reality, literary history, literatures in contact, EDUKA2
Published in RUNG: 18.11.2019; Views: 3622; Downloads: 0
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7.
8.
The importance of not belonging: Paradigmaticity and loan nominalizations in Serbo-Croatian
Marko Simonović, Boban Arsenijević, 2018, original scientific article

Abstract: In a number of Slavic and Germanic languages, various derivational affixes and morphological patterns of Latin origin are relatively common, and bear effects as abstract as deriving event nouns from verbs and property nouns from adjectives. This seems to contradict the general observation that abstract morphology typically is not subject to borrowing. We discuss the status of two Serbo-Croatian (S-C) nominalizing Latinate suffixes, -cija and -itet, complemented by one Germanic suffix, -er. On our analysis, these are not borrowed suffixes and derivational patterns, in the sense that they were present in another language and got copied into S-C, but rather suffixes and patterns which emerged within S-C, more specifically in the borrowed stratum of the S-C lexicon. Crucial factors in their emergence were the shared semantic properties of the nouns ending in the respective sequences (-cija, -itet and -er), and the quantitative properties of these sequences closely matching those of native derivational suffixes. Pragmatic, phonological and prosodic constraints apply to these derivations to the effect that the suffixes that have emerged in the borrowed domain of the lexicon never enter a competition with the native nominalization patterns.
Keywords: nominalisation, borrowing, loanword, language contact, Serbo-Croatian
Published in RUNG: 29.11.2018; Views: 3691; Downloads: 120
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9.
Interface phenomena between CdTe and ZnTe: Cu back contact
Alessio Bosio, Roberta Ciprian, Alessio Lamperti, I Rago, Barbara Ressel, Greta Rosa, Matija Stupar, E Weschke, 2018, original scientific article

Abstract: Thin film technology has reached a maturity to achieve conversion efficiencies of the order of 22%. Among thin films, CdTe-based photovoltaic modules represent 80% of the total production. Nonetheless, some issues concerning back-contact are still open. In industrial process a chemical etching is required in order to make the CdTe film surface rich in Te. The Te-excess is fundamental in order to form a stable telluride compound with copper and to obtain an ohmic, low-resistance back-contact. Moreover, the Te-excess hinders the fast diffusion of copper in CdTe and its achievement of the junction region, preventing the destruction of the device. In this paper we study a ZnTe:Cu buffer layer deposited onto a CdTe film, characterized by a naturally Te-rich surface obtained with a particular chlorine heat treatment without any chemical etching. Copper diffusion and the CdTe/ZnTe:Cu interface were studied by x-ray
Keywords: solar cells, CdTe, ZnTe:Cu back contact
Published in RUNG: 29.11.2018; Views: 4102; Downloads: 0
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10.
Geomorphological characteristics of karst on contact between limestone and dolomite in Slovenia
Petra Gostinčar, 2016, doctoral dissertation

Abstract: Contact karst is a type of karst formed where allogenic waters from the surface influence the karst geomorphic system. Contact karst may be considered in both a strict sense and in a wide sense. In a strict sense, contact karst is the karst phenomena and forms influenced by the contact between a karstifiable rock and a non-karstifiable rock. In a wide sense, contact karst may also be the karst phenomena and forms influenced by the contact between two different karstifiable rocks, for example limestone and dolomite. This thesis focuses on the geomorphological characteristics of contact karst on limestone-dolomite contacts in Slovenia. The purpose of the research was to determine which processes contribute to the development of contact karst on the contact between limestone and dolomite, to define their dynamics, and to identify which surface and underground landforms are developed. The spatial distribution of contacts between limestone and dolomite in Slovenia was determined in a GIS. Using existing lithological data as a data layer, the extent of carbonate rock cover in Slovenia was calculated. Carbonate rocks cover 47 % of Slovenia’s territory (27 % limestone, 14 % dolomite, and 6 % clastic carbonate or impure carbonate rocks). And, there are 1,353 limestone-dolomite contact lines in the country, totalling a length of 2,625 km. Study areas were selected based on GIS analysis of the limestone-dolomite contacts. A total of 17 areas in Slovenia were studied in detail. Fieldwork at the study areas consisted of the collection and analysis of rock, sediment, and water samples, allowing each study area to be geomorphologically mapped. General factors contributing to contact karst development on the lithological contact between limestone and dolomite were determined. The most important factor appears to be the characteristics of the inflow part, formed on the dolomite. Where dolomite functions as a karst rock, the water is dispersedly drained into the karst. In that case, the limestone-dolomite contact does not function as contact karst. Alternatively, where the dolomite functions as fluviokarst, a point recharge, or sinking stream, is formed. In that case, contact karst may be formed. The fluviokarstic character of the dolomite depends on its chemical and mechanical properties. The dolomite bedrock must be positioned at a higher elevation than the neighbouring limestone bedrock. To meet this requirement, dolomite beds, which in Slovenia are generally older than limestone and hence stratigraphically positioned below the limestone beds, need to be positioned above limestone by either folding that leads to inverse stratification, overthrusting, or by displacement along faults. Along faults, the dolomite is more prone to mechanical weathering due to tectonic crushing in addition to its chemical properties. Hence, contact karst is more likely to form at thrust contacts between thrust limestone and dolomite. Limestone-dolomite contact karst develops predominately at higher elevations due to increased precipitation (where allogenic inflow is higher) and greater frost action due to lower temperatures. Intense mechanical weathering of dolomite over limestone directly affects contact karst processes and significantly contributes to the spatial distribution of these types of surfaces. The location of the water table close to the surface is also a leading factor in limestone-dolomite contact karst formation due to enhanced border corrosion. Landforms typical of contact karst were identified in the study areas during geomorphological analyses. However, they are not as clearly recognizable as those on contact between carbonate and non-carbonate rocks. The reason for this is the fact that allogenic waters from dolomitic catchment areas are by far not as corrosive as those from non-carbonate catchment areas.
Keywords: carbonate rocks, dolomite, limestone, contact karst, allogenic water, karst geomorphology
Published in RUNG: 19.12.2016; Views: 7052; Downloads: 263
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