1. Engineering of functional nanosnowflakes from gold nanocarriers capped with amino-modified DNA oligonucleotidesAlexandre Loukanov, Velichka Arahangelova, Saim Emin, Chavdar Filipov, 2023, original scientific article Abstract: Abstract
The design, engineering and electron microscopic characterization of anisotropic nanosized snowflake‐like structural assemblies (nanosnowflakes) is reported. They were fabricated through immobilization of double stranded amine‐modified and thiol‐terminated DNA oligonucleotides on the surface of ultra‐small isotropic gold nanoparticles used as nanocarriers. The transmission electron microscopy images combined with spectrophotometric data revealed the formation of self‐assembled structural aggregation between individual ligands‐coated nanoparticles. They act as seeds for the further spontaneous dendritic growth in different directions. Their anisotropic morphology is formed due to the occurrence of facilitated electrostatic interactions between positive charged amino‐groups and the negative sugar‐phosphate backbone of oligonucleotides. Thus, nanosnowflakes with size distribution between 40 and 80 nm were obtained. The microscopic analysis demonstrated also that the stable nanosnowflakes structure was highly dependent on the solution ionic strength, which effect the charge fluctuation within the assembly. The reported DNA functionalized nanostructures have potential to be applied as a platform for development of therapeutic materials, as well as drug delivery nanosystems.
Research Highlights
The engineering, fabrication, and microscopic characterization of DNA nanosnowflakes is reported.
The electron microscopy analysis revealed formation of self‐assemblies with anisotropic morphology.
The nanosnowflakes size distribution was between 40 and 80 nm.
Keywords: DNA, nanocarrier, functionalization, drug delivery
Published in RUNG: 18.03.2024; Views: 209; Downloads: 0
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2. A New Variant of Dynamic Pickup and Delivery Problem with Time WindowsPetr Valenta, Hana Rudová, Soumen Atta, 2020, published scientific conference contribution Abstract: Motivated by the challenges faced by a logistics company, we present a new variant of the dynamic capacitated pickup and delivery problem with time windows (PDPTW) where excessive changes of unaffected routes are undesirable. In real-life scenarios, different dynamism sources such as canceled requests, change of demands, change of pickup, or delivery time windows often disrupt the existing planning of routes. The static PDPTW is solved with the current information about the problem well before executing the routes, such as the previous night. We present an algorithmic idea of a dynamic solver quickly addressing changes that occur due to the dynamism while avoiding excessive modifications to the previous solution. Since the company has not yet the dynamic data, new dynamic instances are generated from the existing static PDPTW instances in the literature. Preliminary results demonstrate that we can quickly incorporate the required changes. Future perspectives of this ongoing work are discussed in the end.
Keywords: Dynamic Pickup and Delivery Problem, Time Windows, Heuristic
Published in RUNG: 17.04.2023; Views: 1162; Downloads: 0
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