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Abstract: Marine ecosystems are constantly influenced by anthropogenic pressures, including inputs of nutrients, pollutants, and allochthonous microorganisms. These inputs can disrupt the dynamics of marine microbiomes, which encompass the majority of biomass and highest diversity of all life forms, controlling most biogeochemical cycles in the ocean realm. Despite their importance for public safety and potential influence on marine ecosystems functioning, knowledge of the impact of these perturbations on coastal microbiome dynamics remains in its infancy. The research objectives of this dissertation were to: (1) characterize the composition of the coastal microbiome in anthropogenically impacted coastal ecosystem; (2) investigate the genetic potential of selected microorganisms for pathogenicity and ecological adaptations; and (3) explore the effects of wastewater on the dynamics and functioning of the coastal microbiome, as well as its implications for the biogeochemical state of the ecosystem. To address the first aim, we conducted a year-round in situ survey of the pelagic microbiome within anthropogenically impacted coastal ecosystem, focusing on the seasonal and spatial dynamics of traditional and alternative faecal bacterial indicators. We used a culture-independent approach combined with 16S rRNA amplicon sequencing, which overcome limitations of culture-based methods. This analysis revealed that the microbiome was primarily structured by seasonal changes, regardless of proximity to pollution sources. The statistical tool and oceanographic model we applied indicated that riverine water serves as a key vector for introducing allochthonous microbes. This study underscores the importance of molecular approaches combined with statistical and oceanographic modelling for advancing environmental health assessments and detecting microbial indicators. To address the second aim, we applied whole-genome sequencing to assess the pathogenic potential and genomic features of selected Vibrio isolates. During the analysis, we identified cross-contamination in one isolate, which presented an opportunity to evaluate the effectiveness of bioinformatics workflows for contaminant removal and genome recovery. We reconstructed high-quality genomes of one Vibrio isolate from both axenic and contaminated cultures. Genomic analyses revealed that this isolate belongs to a sub-lineage of Vibrio campbellii associated with diseases in marine organisms. Moreover, this genome harboured a novel Vibrio plasmid linked to bacterial defense mechanisms and horizontal gene transfer, potentially offering a competitive advantage to this putative pathogen. This study highlights the utility of WGS and advanced bioinformatics in overcoming challenges posed by non-axenic cultures and provides new insights into the genomic characteristics of V. campbellii. To address the third aim, we conducted a short-term microcosm experiment simulating wastewater discharge into coastal seawater, testing two types of wastewaters: (a) unfiltered, containing nutrients, pollutants, and allochthonous microbes, and (b) pre-filtered, retaining only nutrients and pollutants. Our results showed that wastewater, significantly increased nutrient levels (dissolved organic carbon, ammonium, orthophosphate). Using a multi-omics approach with measurements of microbial metabolic activity, we found that nutrient enrichment significantly influenced bacterial metabolism. This was evidenced by enriched protein profiles and increased leucine aminopeptidase and olease activity, indicating bacterial degradation of complex proteins and lipids. At the same time, the phosphate input resulted in a decreased alkaline phosphatase activity, with important implications for phosphorus cycling. Overall, wastewater primarily induced functional shifts in coastal microbiomes, highlighting the resilience and functional redundancy of coastal microbial communities and hence the biogeochemical processes they operate.
Keywords: coastal microbiome, anthropogenic impact, wastewater pollution, bacterial community dynamics, allochthonous microorganisms, potential pathogens, Vibrio campbellii, multi-omics, whole-genome assembly, dissertations
Published in RUNG: 16.04.2025; Views: 96; Downloads: 0
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Abstract: The discovery of gravitational waves, high-energy neutrinos or the very-high-energy counterpart of gamma-ray bursts has revolutionized the high-energy and transient astrophysics community. The development of new instruments and analysis techniques will allow the discovery and/or follow-up of new transient sources. We describe the prospects for the Cherenkov Telescope Array (CTA), the next-generation ground-based gamma-ray observatory, for multi-messenger and transient astrophysics in the decade ahead. CTA will explore the most extreme environments via very-high-energy observations of compact objects, stellar collapse events, mergers and cosmic-ray accelerators.
Keywords: multi-messenger astrophysics, gravitational waves, very-high-energy (VHE) gamma rays, cosmic rays, VHE neutrinos, transient astrophysical phenomena, Cherenkov Telescope Array Observatory
Published in RUNG: 13.01.2025; Views: 585; Downloads: 7
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Abstract: The birth of gravitational-wave / electromagnetic astronomy was heralded by the joint observation of gravitational waves (GWs) from a binary neutron star (BNS) merger by Advanced LIGO and Advanced Virgo, GW170817, and of gamma-rays from the short gamma-ray burst GRB170817A by the Fermi Gamma-ray Burst Monitor (GBM) and INTEGRAL. This detection provided the first direct evidence that at least a fraction of BNSs are progenitors of short GRBs. GRBs are now also known to emit very-high-energy (VHE, > 100 GeV) photons as has been shown by recent independent detections of the GRBs 1901114C and 180720B by the ground-based gamma-ray detectors MAGIC and H.E.S.S. In the next years, the Cherenkov Telescope Array (CTA) will boost the searches for VHE counterparts thanks to its unprecedented sensitivity, rapid response and capability to monitor large sky areas via survey-mode operation. In this contribution, we present the CTA program of observations following the detection of GW events. We discuss various follow-up strategies and links to multi-wavelength and multi-messenger observations. Finally we outline the capabilities and prospects of detecting VHE emission from GW counterparts.
Keywords: multi-messenger astrophysics, the Cherenkov Telescope Array (CTA) Observatory, gravitational waves, very-high-energy photons, gravitational-wave follow-up program
Published in RUNG: 08.11.2024; Views: 793; Downloads: 5
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Keywords: aerosol absorption coefficient, equivalent black carbon, MAAP, polar photometer, multi-wavelength
Published in RUNG: 13.05.2024; Views: 1429; Downloads: 3
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Keywords: multi-time resolution, source apportionment, submicronic particulate matter, positive matrix factorisation, PMF, multilinear engine, SoFi, ME2, organic sources, metals
Published in RUNG: 24.10.2023; Views: 2279; Downloads: 8
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