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1.
Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers
Pieranna Chiarella, Renata Sisto, Ario de Marco, 2018, original scientific article

Found in: ključnih besedah
Summary of found: ...biomarkers, gene polymorphism, NRF2, oxidative stress, PCR, RFLP...
Keywords: biomarkers, gene polymorphism, NRF2, oxidative stress, PCR, RFLP
Published: 11.12.2018; Views: 2514; Downloads: 119
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2.
Comparison of ex vivo and in vitro human fibroblast ageing models.
Pascal Sommer, Roberta Tiozzo, Giulia Annovi, Federica Boraldi, Daniela Quaglino, 2010, original scientific article

Abstract: Several studies have analyzed modulation of gene expression during physiological ageing with interesting, but often contradictory results, depending on the model used. In the present report we compare age-related metabolic and synthetic parameters in human dermal fibroblasts (HDF) isolated from young and old subjects (ex vivo ageing model) and cultured from early up to late cumulative population doublings (CPD) (in vitro ageing model) in order to distinguish changes induced in vivo by the aged environment and maintained in vitro, from those associated with cell senescence and progressive CPD. Results demonstrate that fibroblasts from aged donors, already at early CPD, exhibit an impaired redox balance, highlighting the importance of this parameter during ageing, even in the presence of standard environmental conditions, which are considered optimal for cell growth. By contrast, several proteins, as those related to heat shock response, or involved in endoplasmic reticulum and membrane trafficking, appeared differentially expressed only during in vitro ageing, suggesting that, at high CPD, the whole cell machinery becomes permanently altered. Finally, given the importance of the elastic component for a long-lasting connective tissue structural and functional compliance, this study focuses also on elastin and fibulin-5 synthesis and deposition, demonstrating a close relationship between fibulin-5 and ageing.
Found in: ključnih besedah
Summary of found: ...Ageing Fibroblast Connective tissue Oxidative stress Protein expression Elastin...
Keywords: Ageing Fibroblast Connective tissue Oxidative stress Protein expression Elastin
Published: 23.08.2019; Views: 2141; Downloads: 0
.pdf Fulltext (830,45 KB)

3.
4.
Under fungal attack on a metalliferous soil: ROS or not ROS? Insights from Silene paradoxa L. growing under copper stress
Cosimo Taiti, Elisabetta Giorni, Ilaria Colzi, Sara Pignattelli, Nadia Bazihizina, Antonella Buccianti, Simone Luti, Luigia Pazzagli, Stefano Mancuso, Cristina Gonnelli, 2016, original scientific article

Abstract: We investigated how the adaptation to metalliferous environments can influence the plant response to biotic stress. In a metallicolous and a non-metallicolous population of Silene paradoxa the induction of oxidative stress and the production of callose and volatiles were evaluated in the presence of copper and of the PAMP fungal protein cerato-platanin, separately and in combination. Our results showed incompatibility between the ordinary ROS-mediated response to fungal attack and the acquired mechanisms of preventing oxidative stress in the tolerant population. A similar situation was also demonstrated by the sensitive population growing in the presence of copper but, in this case, with a lack of certain responses, such as callose production. In addition, in terms of the joint behaviour of emitted volatiles, multivariate statistics showed that not only did the populations respond differently to the presence of copper or biotic stress, but also that the biotic and abiotic stresses interacted in different ways in the two populations. Our results demonstrated that the same incompatibility of hyperaccumulators in ROS-mediated biotic stress signals also seemed to be exhibited by the excluder metallophyte, but without the advantage of being able to rely on the elemental defence for plant protection from natural enemies.
Found in: ključnih besedah
Summary of found: ...population of Silene paradoxa the induction of oxidative stress and the production of callose and...
Keywords: Biotic interactions Callose Heavy metals Oxidative stress VOCs
Published: 20.04.2020; Views: 2022; Downloads: 0
.pdf Fulltext (6,63 MB)

5.
Short-term physiological and biometrical responses of Lepidium sativum seedlings exposed to PET-made microplastics and acid rain
Serena Felline, Antonio Terlizzi, Manuela Piccardo, Andrea Broccoli, Sara Pignattelli, Monia Renzi, 2021, original scientific article

Abstract: Plastics enter in terrestrial natural system primarily by agricultural purposes, while acid rain is the result of anthropogenic activities. The synergistic effects of microplastics and acid rain on plant growth are not known. In this study, different sizes of polyethylene terephthalate (PET) and acid rain are tested on Lepidium sativum, in two separate experimental sets. In the first one we treated plants only with PET, in the second one we used PET and acid rain together. In both experimentations we analyzed: i) plant biometrical parameters (shoot height, leaf number, percentage inhibition of seed germination, fresh biomass), and ii) oxidative stress responses (hydrogen peroxide; ascorbic acid and glutathione). Results carried out from our experiments highlighted that different sizes of polyethylene terephthalate are able to affect plant growth and physiological responses, with or without acid rain supplied during acute toxicity (6 days). Short description: This study showed that different sizes of PET microplastics affect physiological and biometrical responses of Lepidum sativum seedlings, with or without acid rain; roots and leaves responded differently
Found in: ključnih besedah
Summary of found: ...of seed germination, fresh biomass), and ii) oxidative stress responses (hydrogen peroxide; ascorbic acid and glutathione)....
Keywords: Polyethylene terephthalate Acid rain Oxidative stress Biometrical response Lepidium sativum
Published: 16.12.2020; Views: 1253; Downloads: 0
.pdf Fulltext (1,14 MB)

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