1. Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type AnalysisMaryline Pflieger, Petra Makorič, Manca Kovač Viršek, Špela Koren, 2017, review article Abstract: Plastic resin pellets, categorized as microplastics (≤5 mm in diameter), are small granules that can be unintentionally released to the
environment during manufacturing and transport. Because of their environmental persistence, they are widely distributed in the oceans and
on beaches all over the world. They can act as a vector of potentially toxic organic compounds (e.g., polychlorinated biphenyls) and might
consequently negatively affect marine organisms. Their possible impacts along the food chain are not yet well understood. In order to assess the
hazards associated with the occurrence of plastic pellets in the marine environment, it is necessary to develop methodologies that allow for rapid
determination of associated organic contaminant levels. The present protocol describes the different steps required for sampling resin pellets,
analyzing adsorbed organochlorine pesticides (OCPs) and identifying the plastic type. The focus is on the extraction of OCPs from plastic pellets
by means of a pressurized fluid extractor (PFE) and on the polymer chemical analysis applying Fourier Transform-InfraRed (FT-IR) spectroscopy.
The developed methodology focuses on 11 OCPs and related compounds, including dichlorodiphenyltrichloroethane (DDT) and its two main
metabolites, lindane and two production isomers, as well as the two biologically active isomers of technical endosulfan. This protocol constitutes
a simple and rapid alternative to existing methodology for evaluating the concentration of organic contaminants adsorbed on plastic pieces. Keywords: Environmental Sciences, Issue 125, Microplastics, resin pellets, pesticides, persistent organic pollutants, organochlorine pesticides, dichlorodiphenyltrichloroethane, endosulfan, hexachlorocyclohexane, lindane, Fourier transform infrared spectroscopy, pressurized fluid extractor Published in RUNG: 10.07.2017; Views: 5297; Downloads: 0 |
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3. TLS Spectrometry & Detection in Liquid Chromatography, FIA and Microfluidic SystemsMladen Franko, invited lecture at foreign university Abstract: This presentation provides theoretical backround for thermal lens spectrometry (TLS) and TLS microscopy (TLM) and describes the basic instrumentation for TLS and TLM measurements. Presented applications of TLS and TLM include detection in HPLC (carotenoids, bilirubin, biliverdin), FIA (alergens, Ag, Ag+, phytoplankton and cyanobacterial pigments, organophospate pesticides) and TLM detection in microfluidic systems (Cr(VI), microcystin, NGAL, study of diffusion proceses) Keywords: Spekrometry TLS, TLM, HPLC, FIA, microfluidics, bilirubin, biliverdin, carotenoids, alergens, mikrocystin, pigments, organophosphate pesticides, NGAL, Ag, Cr(VI), diffusion Published in RUNG: 16.09.2016; Views: 6036; Downloads: 5 Full text (35,40 MB) |
4. Optimised calibration procedure for bioanalytical determination of organophosphate pesticides in apple juices by immobilised AChEBoris Pihlar, Klavdija Mežnar, Mladen Franko, Lea Pogačnik, 2003, original scientific article Abstract: Optimised calibration procedure for bioanalytical determination of organophosphate pesticides in apple juices by immobilised AChE Keywords: analitske metode, biosenzorji, pesticidi, sadni sokovi, analytical techniques, biosensors, FIA, flow injection analysis, pesticides, fruit juices Published in RUNG: 17.05.2016; Views: 5576; Downloads: 18 Link to full text |
5. Determination of organochlorine pesticides adsorbed on plastic pellets2015, published scientific conference contribution abstract Abstract: In the past years, several studies have revealed the presence of organic contaminants at
concentrations from sub ng g–1 to mg g–1 on/in plastic pellets found in coastal environment
worldwide [1,2,3]. Plastic pellets are actually industrial raw material, typically in the shape of
small granules with a diameter of a few mm. They are categorized as microplastics (< 5 mm).
They can be unintentionally lost in the environment during manufacturing and transport. They
can subsequently reach the marine and coastal compartments. Due to their environmental
persistence, they are widely distributed in the oceans and on beaches [1]. Organic pollutants
associated to pellets are either additives (e.g. PBDEs) that are incorporated into plastics during
production processes or hydrophobic chemicals (e.g. PCBs, organochlorine pesticides) which
adsorb from the surrounding environment (e.g. seawater). Among these chemicals, some are
recognized as POPs (Persistent Organic Pollutants) because of their persistence,
bioaccumulation and potential adverse environmental and health effects. Thus, in order to
better assess the impact of plastic pellets in coastal environment, it is necessary to determine
the level of associated organic pollutants.
The present study was carried out in the frame of DeFishGear project, which focuses on marine
litter and microplastics issues in Adriatic region. This investigation aimed at developing an
experimental protocol allowing the quantification of 11 organochlorine pesticides. Plastic
pellets, sampled on beaches located in the Adriatic region, were first sorted by colour. Pesticides
were extracted from the plastic matrix (c.a. 0.5 g) in a pressurized fluid extractor (50°C, 100 bar).
Prior to evaporation, the extract was cleaned on Florisil sorbent through solid-phase extraction
(SPE). The concentrated extracts were quantified on gas chromatography equipped with a micro
electron capture detector (GC-μECD). Preliminary results revealed the presence of DDT
transformation products. Keywords: organochlorine pesticides, microplastics, pellets, Adriatic region Published in RUNG: 15.02.2016; Views: 5367; Downloads: 0 This document has many files! More... |