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Keywords: Two-phase flow, free and moving boundaries, computational fluid dynamics, phasefield formulation, 2D problems, axisymmetric problems, diffuse approximate meshless method, Rayleigh-Taylor instability, Boussinesq approximation, variable density and viscosity, flow focusing, dripping, jetting
Published in RUNG: 11.09.2018; Views: 5058; Downloads: 178  (1 vote)
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Abstract: A series of fixed frequency dielectric measurements shows dielectric relaxation in La2CuMnO6, with a dynamic (Arrhenius to Arrhenius) crossover at TC. The external magnetic field alters the relaxation parameters in the vicinity of crossover and induces an unusual trend in the magnetodielectric coupling around TC. A large magnetodielectric coupling of 55% (at 68 K, 4 kHz) and 61% (at 105 K, 285.8 kHz) under a small magnetic field of 5 kOe is discovered. Presence of ferromagnetic short-range correlations above TC and a sign reversal of magnetoresistance around TC are observed. Specific heat analysis revealed the presence of ferromagnetic, variable range hopping active charge localized state. The presence of ferromagnetic short-range correlations and the influence of a core-grain dominated magnetoresistance on the Maxwell-Wagner interfacial polarization are responsible for the observed large magnetodielectric effect. Both the magnetic ordering and external magnetic field control the electric dipole relaxation in the material.
Keywords: Double perovskite Griffiths-like phase Dielectric Magnetoresistance Magnetodielectric
Published in RUNG: 03.01.2018; Views: 3755; Downloads: 0
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Abstract: Irinotecan is currently used in several cancer regimens mainly in colorectal cancer (CRC). This drug has a narrow therapeutic range and treatment can lead to side effects, mainly neutropenia and diarrhea, frequently requiring discontinuing or lowering the drug dose. A wide inter-individual variability in irinotecan pharmacokinetic parameters and pharmacodynamics has been reported and associated to patients’ genetic background. In particular, a polymorphism in the UGT1A1 gene (UGT1A1*28) has been linked to an impaired detoxification of SN-38 (irinotecan active metabolite) to SN-38 glucuronide (SN-38G) leading to increased toxicities. Therefore, therapeutic drug monitoring of irinotecan, SN-38 and SN-38G is recommended to personalize therapy. In order to quantify simultaneously irinotecan and its main metabolites in patients’ plasma, we developed and validated a new, sensitive and specific HPLC–MS/MS method applicable to all irinotecan dosages used in clinic. This method required a small plasma volume, addition of camptothecin as internal standard and simple protein precipitation. Chromatographic separation was done on a Gemini C18 column (3 μM, 100 mm x 2.0 mm) using 0.1% acetic acid/bidistilled water and 0.1% acetic acid/acetonitrile as mobile phases. The mass spectrometer worked with electrospray ionization in positive ion mode and selected reaction monitoring. The standard curves were linear (R20.9962) over the concentration ranges (10–10000 ng/mL for irinotecan, 1–500 ng/mL for SN-38 and SN-38G and 1–5000 ng/mL for APC) and had good back-calculated accuracy and precision. The intra- and inter-day precision and accuracy, determined on three quality control levels for all the analytes, were always <12.3% and between 89.4% and 113.0%, respectively. Moreover, we evaluated this bioanalytical method by re-analysis of incurred samples as an additional measure of assay reproducibility. This method wassuccessfully applied to a pharmacokinetic study in metastatic CRC patients enrolled in a genotype-guided phase Ib study of irinotecan administered in combination with 5-fluorouracil/ leucovorin and bevacizumab.
Keywords: Pharmacokinetic, pharmacodynamics, phase I clinical study, colorectal cancer, Mass spectrometry, pharmacogenetics
Published in RUNG: 21.03.2017; Views: 4559; Downloads: 0
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Abstract: In spin models, that are applied to describe the conformational transitions in polymers, the number of spin orientations, that correspond to the disordered conformation, can be estimated using fundamental definitions of Statistical Physics. For instance, when considering alpha-helix to coil transition in polypeptides, the role of generalized coordinates is played by pairs of torsional angle, and the repeating unit populates different regions of that 2D contour map, depending on conformation. By scanning over all possible torsional angles, that do not violate the obvious limitations due to the excluded volume, the so-called Ramachandran map can be plotted, which is actually the phase space visualization for the helix-coil transition problem. The region of phase space, corresponding to the ordered, helical conformations, is much more limited, than the one, corresponding to all other (allowed) conformations. We can calculate the areas of these regions as Γhelix and Γcoil , and construct the ratio Q = Γcoil . Naturally, it can be interpreted as log(Q) = Scoil − Shelix = ΔS, the entropic cost of helix with respect to coil. To illustrate the importance of the entropic price of ordered conformation we report our recent results, that allowed to explain the peculiarity of phase diagrams of Intrinsically Disordered Proteins (IDP) out of larger Q-values, as compared to globular counterparts. In particular, it has been shown, that due to larger Q, the phase diagram of IDP is shifted towards higher temperatures.
Keywords: IDP, protein folding, phase diagram
Published in RUNG: 23.06.2016; Views: 4606; Downloads: 0
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