2. Connective tissue and diseases: from morphology to proteomics towards the development of new therapeutic appproachDaniela Quaglino, Federica Boraldi, Giulia Annovi, Deanna Guerra, Ivonne Pasquali Ronchetti, 2009, review article Abstract: Connective tissue consists of cells separated by the extracellular matrix, whose composition and amount vary according
to age, to functional requirements, and to the presence of pathologic conditions. Within this non-random
macromolecular assembly, collagens, elastin, proteoglycans and structural glycoproteins are mutually interdependent
and modifications of one component, by extrinsic (environmental) and/or intrinsic (systemic, genetic, age-related)
factors, may have consequences on the tissue as a whole. Since decades, different microscopical techniques have been
applied mainly for diagnostic purposes and for detailed descriptions of changes occurring in cells and in matrix
components. More recently, in order to dissect the molecular complexity of the matrix network, to analyse the
interactions between cells and matrix and to look for modulators of cell phenotype, histomorphologic investigations
have been implemented with proteomic studies that allow to identify possible diagnostic markers, and to better
understand patho-mechanisms enabling the design of novel therapeutic strategies. Therefore, the progressively
expanding, although incomplete, knowledge on connective tissue biology, sheds new light on the pathogenesis of
diseases affecting single molecules (i.e. collagenopathies, mucopolysaccharidoses, elastinopathies) and discloses the
importance of matrix components as fundamental regulators of cell phenotype, in relation, for instance, to the aging
process and/or to cancer development and progression. Few examples will be presented demonstrating the promises of
proteomics as a technique leading to the discovery of new therapies and possibly to the development of individualized
treatments for a better patient care. Keywords: pathology, proteomics, fibrosis, rheumatology, cancer Published in RUNG: 23.08.2019; Views: 3896; Downloads: 0 This document has many files! More... |
3. New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach.Davide Malagoli, Federica Boraldi, Giulia Annovi, Daniela Quaglino, Enzo Ottaviani, 2009, original scientific article Abstract: Autophagy is an evolutionary ancient process based on the activity of genes conserved from yeast to metazoan taxa. Whereas its role as a mechanism to provide energy during cell starvation is commonly accepted, debate continues about the occurrence of autophagy as a means specifically activated to achieve cell death. The IPLB-LdFB insect cell line, derived from the larval fat body of the lepidoptera Lymantria dispar, represents a suitable model to address this question, as both autophagic and apoptotic cell death can be induced by various stimuli. Using morphological and functional approaches, we have observed that the culture medium conditioned by IPLB-LdFB cells committed to death by the ATPase inhibitor oligomycin A stimulates autophagic cell death in untreated IPLB-LdFB cells. Moreover, proteomic analysis of the conditioned media suggests that, in IPLB-LdFB cells, oligomycin A promotes a shift towards lipid metabolism, increases oxidative stress and specifically directs the cells towards autophagic activity. Keywords: Autophagic cell death, Fat body, IDGF, IPLB-LdFB, Proteomics Published in RUNG: 23.08.2019; Views: 3872; Downloads: 0 This document has many files! More... |