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Measurement campaign of Icelandic volcanic ash during its arrival to Slovenia in April 2010
Fei Gao, Samo Stanič, Klemen Bergant, Tanja Bolte, Franco Coren, Tingyao He, Andrej Hrabar, Ana Mladenovič, Janja Turšič, Darko Veberič, 2011, objavljeni povzetek znanstvenega prispevka na konferenci

Najdeno v: osebi
Ključne besede: vulkanski pepel, atmosfera, daljinsko zaznavanje
Objavljeno: 15.10.2013; Ogledov: 4075; Prenosov: 92
URL Polno besedilo (0,00 KB)

An RNA-based therapeutic approach for inherited mis-splicing diseases
Andrea Dal Mas, 2014, doktorska disertacija

Najdeno v: osebi
Ključne besede: eksoni, genetika, mutacije, spajanje, disertacije
Objavljeno: 22.01.2015; Ogledov: 2687; Prenosov: 85
URL Polno besedilo (0,00 KB)
Gradivo ima več datotek! Več...

Katarzyna Rajkowska, 2018, doktorska disertacija

Opis: The experimental work of this thesis was performed at the International Centre for Genetic Engineering and Biotechnology (ICGEB) in the Human Molecular Genetics Group, under the scientific direction of Prof. Franco Pagani. The project was developed during the academic years 2014-2017. Modified U1 RNAs, also named Exon Specific U1s (ExSpeU1s) represent a novel class of small RNA-based molecules that correct exons splicing defects. To evaluate their therapeutic potential focused on Familial Dysautonomia (FD), a rare autosomal recessive disorder characterized by progressive degeneration of the sensory and autonomic nervous system. More than 99% of patients are homozygous for the T to C transition in position 6 of the IKBKAP intron 20 (c.2204+6T>C). This substitution modifies the exon 20 5’ splice site (5’ss) inducing exon skipping in a tissue-specific manner and reducing the total amount of IKAP protein. The molecular mechanisms underlying the IKBKAP mis-splicing are not completely clear and there are no effective treatments. In this thesis, I investigated the therapeutic potential of ExSpeU1s and the role of cis- and trans-acting factors that regulates IKBKAP splicing. Using a splicing functional assay, I identified ExSpeU1s that bind to intron 20 sequences and rescue the exon 20 skipping defect. Interestingly, their rescue activity was modulated by several splicing factors and requires a critical exonic splicing enhancer element. Transfection experiment showed the involvement of both enhancing (TIA1, PTBP1 and PTB4) and inhibitory (SRSF3, hnRNPA1, FOX and FUS) splicing factors in IKBKAP splicing. To better evaluate the ExSpeU1s therapeutic efficacy, I transduced FD patient’s fibroblasts with a lentiviral vector expressing the most active ExSpeU1. This resulted in a complete rescue of the exon skipping defect and improvement in IKAP protein expression. Most importantly, intraperitoneal delivery of ExSpeU1s by AAV9 into a transgenic mouse model, that recapitulates the tissue-specific mis-splicing seen in FD patients, corrected the aberrant splicing patterns in several tissues increasing the amount of the corresponding IKAP protein. All together, these results identify novel regulatory splicing factors involved in the IKBKAP exon 20 regulation and provide the proof of principle that ExSpeU1s delivered in vivo by AAV vectors represent a novel therapeutic strategy for FD.
Najdeno v: osebi
Povzetek najdenega: ...Group, under the scientific direction of Prof. Franco Pagani. The project was developed during the academic...
Ključne besede: Familial Dysautonomia, IKBKAP, IKAP, splicing, splicing defects, ExSpeU1, U1 snRNA, mouse model, AAV
Objavljeno: 26.03.2018; Ogledov: 2567; Prenosov: 73
.pdf Polno besedilo (13,96 MB)

Lotta guidata in viticoltura
Giorgio Malossini, Paolo Sivilotti, M. Stocco, Giulio Franco, Carlo Frausin, 2012, druge monografije in druga zaključena dela

Najdeno v: osebi
Ključne besede: vinska trta, grozdje, škodljivci, plesni
Objavljeno: 15.10.2013; Ogledov: 4774; Prenosov: 20
URL Polno besedilo (0,00 KB)

XAS and micro-XRF analysis of mono and bi-metallic exopolysaccharide (FePd-EPS) bio-generated by K. oxytoca
Franco Baldi, Katarina Vogel-Mikuš, Michele Gallo, Oreste Piccolo, Stefano Paganelli, Iztok Arčon, 2016, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: Bacteria Klebsiella oxytoca (DSM 29614) secret a specific exopolysaccharide (EPS) in the presence of Fe(III)-citrate, as sole carbon and energy source, and produces after 7 days an iron gel precipitate (Fe-EPS) [1]. In the presence of palladium or other metal species, the culture of K. oxytoca can produce other mono or bimetal species (Pd-EPS, FePd-EPS) [2]. These bio-materials may be used as green biogenerated catalysts or for other biotechnological purposes. In this work we present the analysis of the morphology and the chemical state of the metals in mono and bi-metallic (Fe-EPS, Pd-EPS, FePd-EPS) complexes, by a combination of micro X-ray fluorescence, X-ray absorption spectroscopy methods (XANES and EXAFS), and transmission electron microscopy. The results show that iron in monometallic Fe-EPS and bimetalic FePd-EPS is in the mineralized form of iron oxides/hydroxides, predominantly in the form of Fe3+, with a small amount of Fe2+ in the structure, as a mixture of different nano-crystalline iron oxides and hydroxides. Palladium on the other hand is found as Pd(0) in the form of metallic nanoparticles with fcc structure in both, bi-metallic (FePd-EPS) and mono-metallic (Pd-EPS) species. In bi-metallic species Pd and Fe nanoparticles agglomerate in larger clusters, but they remain spatially separated. Access to the SR facilities at ESRF (beamline ID21, project LS-2225), DESY (beamline C) Hamburg (project I-20110511 EC) and ELETTRA (beamline XAFS, project 20115112) is acknowledged.
Najdeno v: osebi
Ključne besede: Klebsiella oxytoca, Fe, Pd, XANES, EXAFS, exopolysaccharide, FePd-EPS
Objavljeno: 28.06.2016; Ogledov: 3717; Prenosov: 0
.pdf Polno besedilo (388,27 KB)

Alternative Recovery and Valorization of Metals from Exhausted Catalytic Converters in a New Smart Polymetallic Catalyst
Oreste Piccolo, Laura Sperni, Michele Gallo, Katarina Vogel-Mikuš, Iztok Arčon, Franco Baldi, Sebastiano Tieuli, Stefano Paganelli, 2019, izvirni znanstveni članek

Opis: A new metals-polymeric composite, Metx-EPS (I), was prepared to be used as catalyst in water or in two-phase aqueous conditions. The metals source was an exhausted catalytic converter that was grinded and treated with an acidic solution at room temperature. After filtration, the solution was concentrated, neutralized and added to a broth of Klebsiella oxytoca DSM 29614 to produce (I) where metals are embedded in a peculiar polysaccharide structure (EPS). The composite was easily recovered from the fermentation broth and purified. The process protocol was verified many times and was shown to be reproducible satisfactorily. The % recovery of metals, originally present in the converter, was good as determined by atomic absorption. The morphology and the chemical state of main metals in (I) were investigated by X-ray absorption spectroscopy methods (XANES and EXAFS). No metallic alloy seems to be evident. The catalytic activity and a possible synergic effect due to the presence of the different metals was valuated in the hydrogenation of some substrates, valuable precursors for theproduction of fine chemicals.
Najdeno v: osebi
Ključne besede: Biogenerated polymetallic exopolysaccharide, biphasic catalysis, hydrogenation, metals-polymeric composite, new catalyst from metallic wastes, EXAFS, XANES
Objavljeno: 06.05.2019; Ogledov: 1385; Prenosov: 0
.pdf Polno besedilo (2,87 MB)

A Biogenerated Polymetallic Catalyst from Society's Wastes
Oreste Piccolo, Stefano Paganelli, Petro Zanatta, Sebastiano Tieuli, Laura Sperni, Franco Baldi, Iztok Arčon, Michele Gallo, Katarina Vogel-Mikuš, 2019, izvirni znanstveni članek

Opis: Aims:Preparation of the new metals - polymeric composite, Met x-EPS (I), to be used as a green catalyst in water or in two-phase aqueous conditions. Study Design: Recovery and valorization of polymetallic wastes to obtain directly new catalysts using a microorganism to explore their application in removal of difficult and dangerous chemical pollutants present in aqueous environment
Najdeno v: osebi
Ključne besede: Metals - polymeric composite, biogenerated polymetallic exopolysaccharide, new catalyst from metallic wastes, hydrodechlorination of PCBs in water
Objavljeno: 06.05.2019; Ogledov: 1532; Prenosov: 0
.pdf Polno besedilo (373,55 KB)

An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groups
Claudia Faleri, Dario Battistel, Salvatore Daniale, Michele Gallo, Franco Baldi, Alojz Kodre, Iztok Arčon, 2017, izvirni znanstveni članek

Opis: A strain of Klebsiella oxytoca DSM 29614 is grown on sodium citrate in the presence of 50 mg l−1 of Hg as Hg(NO3)2. During growth, the strain produces an extracellular polymeric substance (EPS), constituted by a mixture of proteins and a specific exopolysaccharide. The protein components, derived from the outer membrane of cells, are co-extracted with the extracellular exopolysaccharide using ethanol. The extracted EPS contains 7.5% of Hg (total amount). This indicates that EPS is an excellent material for the biosorption of Hg2+, through chemical complexation with the EPS components. The binding capacity of these species towards Hg2+ is studied by cyclic voltammetry, and Hg L3-edge XANES and EXAFS spectroscopy. The results found indicate that Hg2+ is mainly bound to the nitrogen of the imidazole ring or other N-heterocycle compounds. The hydroxyl moities of sugars and/or the carboxyl groups of two glucuronic acids in the polysaccharide can also play an important role in sequestring Hg2+ ions. However, N-heterocyclic groups of proteins bind Hg2+ faster than hydroxyl and carboxyl groups of the polysaccharide.
Najdeno v: osebi
Ključne besede: Klebsiella oxytoca, Biosorption, Proteins, Polysaccharide, Cyclic voltammetry, EXAFS analysis
Objavljeno: 03.03.2017; Ogledov: 2766; Prenosov: 0
.pdf Polno besedilo (1,66 MB)

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