1. Common targetable biomarkers in canine hemangiosarcoma and human angiosarcomaHuyen Thuc Tran Luong, Dominique Revets, Matteo De March, Sofie Vercammen, Hilde De Rooster, Ario De Marco, Antonio Cosma, 2024, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: comparative oncology, angiosarcoma, hemangiosarcoma, nanobodies
Objavljeno v RUNG: 18.03.2025; Ogledov: 107; Prenosov: 0
Gradivo ima več datotek! Več... |
2. Development of molecular methods for the comprehensive evaluation of stress conditions affecting forest trees : dissertationClaudia D'Ercole, 2025, doktorska disertacija Ključne besede: protein complexes, adhirons, nanobodies, plant stress, immunoreagents, protoplasts, ascorbate peroxidase, dissertations
Objavljeno v RUNG: 04.02.2025; Ogledov: 467; Prenosov: 2
 Celotno besedilo (4,89 MB) |
3. |
4. |
5. Computational study of the HLTF ATPase remodeling domain suggests its activity on dsDNA and implications in damage toleranceMartin Ljubič, Claudia D'Ercole, Yossma Waheed, Ario De Marco, Jure Borišek, Matteo De March, 2024, izvirni znanstveni članek Ključne besede: DNA repairing mechanism, protein modeling, chromatin remodeler, helicase-like transcription factor
Objavljeno v RUNG: 15.11.2024; Ogledov: 836; Prenosov: 2
Celotno besedilo (5,17 MB) |
6. |
7. |
8. |
9. Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertationGbenga Folorunsho Oginni, 2024, doktorska disertacija Opis: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Ključne besede: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Objavljeno v RUNG: 10.09.2024; Ogledov: 1103; Prenosov: 17
Celotno besedilo (3,80 MB) |
10. |
