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132. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodiesSandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, izvirni znanstveni članek Opis: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at
lower cost. Here, we describe the first fully synthetic phage display library of humanized llama
single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic
single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse
library provides high affinity binders without animal immunization. NaLi-H1 was screened following
several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1).
Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were
used in various immunoassays and were often found to be functional intrabodies, enabling tracking
or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein
knockdown in living cells. Finally, direct selection against the surface of tumor cells produced
hs2dAb directed against tumor-specific antigens further highlighting the potential use of this
library for therapeutic applications.
Ključne besede: nanobodies, synthetic phage display library, in vitro panning
Objavljeno v RUNG: 17.08.2016; Ogledov: 6928; Prenosov: 247
 Celotno besedilo (4,32 MB) |
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135. An intrabody specific for the nucleophosmin carboxy-terminal mutant and fused to a nuclear localization sequence binds its antigen but fails to relocate it in the nucleusArio De Marco, Chiara Martinelli, Emanuela Colombo, Daniele Piccini, Cristina Sironi, Pier Giuseppe Pelicci, 2014, izvirni znanstveni članek Ključne besede: intrabody, leukemia, protein shuttling, recombinant fusion protein, single-chain antibody
Objavljeno v RUNG: 16.06.2016; Ogledov: 5135; Prenosov: 25
 Povezava na celotno besedilo |
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138. Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodiesArio De Marco, Selma Djender, Anne Beugnet, Aurelie Schneider, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, 2014, izvirni znanstveni članek Ključne besede: recombinant antibodies, cytoplasmic expression, functional tags
Objavljeno v RUNG: 16.06.2016; Ogledov: 5413; Prenosov: 134
Povezava na celotno besedilo |
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