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Isolation of adhirons specific for plant protoplast membrane biomarkers is simplified by phagemid design
Claudia D'Ercole, Ario De Marco, 2023, samostojni znanstveni sestavek ali poglavje v monografski publikaciji

Ključne besede: pea protoplasts, ALFAtag, customized reagents, adhirons, protoplast biomarkers
Objavljeno v RUNG: 19.07.2023; Ogledov: 706; Prenosov: 1
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Real-time monitoring of Arundo donax response to saline stress through the application of in vivo sensing technology
Janni Michela, Claudia Cocozza, Federico Brilli, Sara Pignattelli, Filippo Vurro, Nicola Coppede, Manuele Bettelli, Davide Calestani, Francesco Loreto, Andrea Zappettini, 2021, izvirni znanstveni članek

Opis: One of the main impacts of climate change on agriculture production is the dramatic increase of saline (Na+) content in substrate, that will impair crop performance and productivity. Here we demonstrate how the application of smart technologies such as an in vivo sensor, termed bioristor, allows to continuously monitor in real-time the dynamic changes of ion concentration in the sap of Arundo donax L. (common name giant reed or giant cane), when exposed to a progressive salinity stress. Data collected in vivo by bioristor sensors inserted at two different heights into A. donax stems enabled us to detect the early phases of stress response upon increasing salinity. Indeed, the continuous time-series of data recorded by the bioristor returned a specific signal which correlated with Na+ content in leaves of Na-stressed plants, opening a new perspective for its application as a tool for in vivo plant phenotyping and selection of genotypes more suitable for the exploitation of saline soils.
Ključne besede: Arundo donax, saline stress, vivo sensing technology
Objavljeno v RUNG: 17.12.2021; Ogledov: 1667; Prenosov: 18
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"Umetnost je zgodovina napak, ki pa so poetične, ki delujejo kot nekaj izvirnega. Za vsemi napakmi se skrivajo zgodbe." : "UMETNOST JE ZGODOVINA NAPAK, KI PA SO POETIČNE, KI DELUJEJO KOT NEKAJ IZVIRNEGA. ZA VSEMI NAPAKMI SE SKRIVAJO ZGODBE."
2021, radijska ali televizijska oddaja, podkast, intervju, novinarska konferenca

Opis: Med gosti letošnjega festivala Fabula – Literature sveta je leta 1984 rojena italijanska pisateljica Claudia Durastanti. V slovenščino imamo preveden njen roman Tujka, avtofikcijsko pripoved, ki se začne z odraščanjem ob gluhih starših; odvija se od New Yorka, prek revne južne Italije do Rima in Londona – hkrati pa gre za razmisleke o identiteti, umetnosti in življenju.
Ključne besede: italijanske pisateljice, romani, intervjuji
Objavljeno v RUNG: 05.11.2021; Ogledov: 1501; Prenosov: 6
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"To je v resnici knjiga, ki je nisem hotela napisati" : nenavadna zgodba odraščanja v kaosu jezikov
2021, intervju

Opis: Italijanska pisateljica Claudia Durastanti v romanu Tujka pripoveduje o svoji neobičajni mladosti. Rodila se je v nekonvencionalni družini, njena starša sta imela okvaro sluha, a je nista nikoli naučila uporabljati znakovnega jezika.
Ključne besede: romani, Tujka
Objavljeno v RUNG: 05.11.2021; Ogledov: 1478; Prenosov: 9
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Silver nanoparticles enter the tree stem faster through leaves than through roots
Claudia Cocozza, Annalisa Perone, Cristiana Giordano, Maria Cristina Salvatici, Sara Pignattelli, Aida Raio, Marcus Schaub, Kruno Sever, John L. Innes, Roberto Tognetti, Paolo Cherubini, 2019, izvirni znanstveni članek

Opis: A major environmental pollution problem is the release into the atmosphere of particulate matter, including nanoparticles (NPs), which causes serious hazards to human and ecosystem health, particularly in urban areas. However, knowledge about the uptake, translocation and accumulation of NPs in plant tissues is almost completely lacking. The uptake of silver nanoparticles (Ag-NPs) and their transport and accumulation in the leaves, stems and roots of three different tree species, downy oak (Quercus pubescens Willd.), Scots pine (Pinus sylvestris L.) and black poplar (Populus nigra L.), were assessed. In the experiment, Ag- NPs were supplied separately to the leaves (via spraying, the foliar treatment) and roots (via watering, the root treatment) of the three species. Uptake, transport and accumulation of Ag were investigated through spectroscopy. The concentration of Ag in the stem was higher in the foliar than in the root treatment, and in poplar more than in oak and pine. Foliar treatment with Ag-NPs reduced aboveground biomass and stem length in poplars, but not in oaks or pines. Species-specific signals of oxidative stress were observed; foliar treatment of oak caused the accumulation of H2O2 in leaves, and both foliar and root treatments of poplar led to increased O2− in leaves. Ag-NPs affected leaf and root bacteria and fungi; in the case of leaves, foliar treatment reduced bacterial populations in oak and poplar and fungi populations in pine, and in the case of roots, root treatment reduced bacteria and increased fungi in poplar. Species-specific mechanisms of interaction, transport, allocation and storage of NPs in trees were found. We demonstrated definitively that NPs enter into the tree stem through leaves faster than through roots in all of the investigated tree species.
Ključne besede: Ag-NPs, pathway of uptake, Pinus sylvestris L., Populus nigra L., Quercus pubescens Willd.
Objavljeno v RUNG: 20.04.2020; Ogledov: 2864; Prenosov: 0
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Impact of high or low levels of phosphorus and high sodium in soils on productivity and stress tolerance of Arundo donax plants
Claudia Cocozza, Federico Brilli, Laura Miozzi, Sara Pignattelli, Silvia Rotunno, Cecilia Brunetti, Cristiana Giordano, Susanna Pollastri, Mauro Centritto, Gian Paolo Accotto, Roberto Tognetti, Francesco Loreto, 2019, izvirni znanstveni članek

Opis: The potential of Arundo donax to grow in degraded soils, characterized by excess of salinity (Na+), and phosphorus deficiency (-P) or excess (+P) also coupled with salinity (+NaP), was investigated by combining in vivo plant phenotyping, quantification of metabolites and ultrastructural imaging of leaves with a transcriptome-wide screening. Photosynthesis and growth were impaired by+Na, -P and+NaP. While+Na caused stomatal closure, enhanced biosynthesis of carotenoids, sucrose and isoprene and impaired anatomy of cell walls, +P negatively affected starch production and isoprene emission, and damaged chloroplasts. Finally, +NaP largely inhibited photosynthesis due to stomatal limitations, increased sugar content, induced/repressed a number of genes 10 time higher with respect to+P and+Na, and caused appearance of numerous and large plastoglobules and starch granules in chloroplasts. Our results show that A. donax is sensitive to unbalances of soil ion content, despite activation of defensive mechanisms that enhance plant resilience, growth and biomass production of A. donax under these conditions.
Ključne besede: Abiotic stress Giant reed Isoprene emission Phosphorus Salinity Transcriptome
Objavljeno v RUNG: 20.04.2020; Ogledov: 2753; Prenosov: 0
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The excess of phosphorus in soil reduces physiological performances over time but enhances prompt recovery of salt-stressed Arundo donax plants
Claudia Cocozza, Federico Brilli, Sara Pignattelli, Susanna Pollastri, Cecilia Brunetti, Cristina Gonnelli, Roberto Tognetti, Mauro Centritto, Francesco Loreto, 2020, izvirni znanstveni članek

Opis: Arundo donax L. is an invasive grass species with high tolerance to a wide range of environmental stresses. The response of potted A. donax plants to soil stress characterized by prolonged exposure (43 days) to salinity (+Na), to high concentration of phosphorus (+P), and to the combination of high Na and P (+NaP) followed by 14 days of recovery under optimal nutrient solution, was investigated along the entire time-course of the experiment. After an exposure of 43 days, salinity induced a progressive decline in stomatal conductance that hampered A. donax growth through diffusional limitations to photosynthesis and, when combined with high P, reduced the electron transport rate. Isoprene emission from A. donax leaves was stimulated as Na+ concentration raised in leaves. Prolonged growth in P-enriched substrate did not significantly affect A. donax performance, but decreased isoprene emission from leaves. Prolonged exposure of A. donax to + NaP increased the leaf level of H2O2, stimulated the production of carbohydrates, phenylpropanoids, zeaxanthin and increased the de-epoxidation state of the xanthophylls. This might have resulted in a higher stress tolerance that allowed a fast and full recovery following stress relief. Moreover, the high amount of ABA-glucose ester accumulated in leaves of A. donax exposed to + NaP might have favored stomata re-opening further sustaining the observed prompt recovery of photosynthesis. Therefore, prolonged exposure to high P exacerbated the negative effects of salt stress in A. donax plants photosynthetic performances, but enhanced activation of physiological mechanisms that allowed a prompt and full recovery after stress.
Ključne besede: Arundo donax Phosphorus Salinity Stress tolerance Biomass production
Objavljeno v RUNG: 20.04.2020; Ogledov: 2923; Prenosov: 0
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