1. Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitorsRonan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, izvirni znanstveni članek Opis: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity
and capacity to recognize their native antigen are critical characteristics for their in vivo application.
Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and
spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody
suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe
the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly
panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii)
show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope
with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules
in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to
identify useful functional antibodies for further members of the GPCR class.
Ključne besede: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Objavljeno v RUNG: 19.10.2017; Ogledov: 4908; Prenosov: 0
Gradivo ima več datotek! Več... |
2. Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived XenograftsRonan Crepin, David Gentien, Angeline Duche, Audrey Rapinat, Cecile Reyes, Fariba Nemati, Gerald Massonnet, Didier Deacaudin, Selma Djander, Sandrine Moutel, Klervi Even Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman-Roman, Ario De Marco, 2017, izvirni znanstveni članek Opis: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Ključne besede: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Objavljeno v RUNG: 19.04.2017; Ogledov: 6399; Prenosov: 0
Gradivo ima več datotek! Več... |
3. NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodiesSandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario De Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, izvirni znanstveni članek Opis: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at
lower cost. Here, we describe the first fully synthetic phage display library of humanized llama
single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic
single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse
library provides high affinity binders without animal immunization. NaLi-H1 was screened following
several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1).
Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were
used in various immunoassays and were often found to be functional intrabodies, enabling tracking
or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein
knockdown in living cells. Finally, direct selection against the surface of tumor cells produced
hs2dAb directed against tumor-specific antigens further highlighting the potential use of this
library for therapeutic applications.
Ključne besede: nanobodies, synthetic phage display library, in vitro panning
Objavljeno v RUNG: 17.08.2016; Ogledov: 6396; Prenosov: 247
 Celotno besedilo (4,32 MB) |
4. Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodiesArio De Marco, Selma Djender, Anne Beugnet, Aurelie Schneider, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, 2014, izvirni znanstveni članek Ključne besede: recombinant antibodies, cytoplasmic expression, functional tags
Objavljeno v RUNG: 16.06.2016; Ogledov: 5131; Prenosov: 134
 Povezava na celotno besedilo |
