1. Biological applications of synthetic binders isolated from a conceptually new adhiron libraryClaudia D'Ercole, Matteo De March, Gianluca Veggiani, Sandra Oloketuyi, Rossella Svigelj, Ario De Marco, 2023, izvirni znanstveni članek Ključne besede: synthetic library, adhirons, affimers, C-reactive protein, SpyCatcher Objavljeno v RUNG: 17.10.2023; Ogledov: 1599; Prenosov: 4 Celotno besedilo (2,23 MB) Gradivo ima več datotek! Več... |
2. Comparative analysis of fusion tags used to functionalize recombinant antibodiesArio De Marco, Gianluca Veggiani, Barbara Giabbai, Marta Semrau, Barbara Medagli, Vincenzo Riccio, Gregor Bajc, Paola Storici, 2020, izvirni znanstveni članek Ključne besede: recombinant antibodies, protein production, fusion tags Objavljeno v RUNG: 30.09.2019; Ogledov: 3683; Prenosov: 71 Celotno besedilo (740,27 KB) |
3. Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitorsRonan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, izvirni znanstveni članek Opis: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity
and capacity to recognize their native antigen are critical characteristics for their in vivo application.
Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and
spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody
suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe
the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly
panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii)
show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope
with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules
in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to
identify useful functional antibodies for further members of the GPCR class. Ključne besede: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library Objavljeno v RUNG: 19.10.2017; Ogledov: 4796; Prenosov: 0 Gradivo ima več datotek! Več... |