21. Silver nanoparticles enter the tree stem faster through leaves than through rootsClaudia Cocozza, Annalisa Perone, Cristiana Giordano, Maria Cristina Salvatici, Sara Pignattelli, Aida Raio, Marcus Schaub, Kruno Sever, John L. Innes, Roberto Tognetti, Paolo Cherubini, 2019, izvirni znanstveni članek Opis: A major environmental pollution problem is the release into the atmosphere of particulate matter, including nanoparticles (NPs), which causes serious hazards to human and ecosystem health, particularly in urban areas. However, knowledge about the uptake, translocation and accumulation of NPs in plant tissues is almost completely lacking. The uptake of silver nanoparticles (Ag-NPs) and their transport and accumulation in the leaves, stems and roots of three different tree species, downy oak (Quercus pubescens Willd.), Scots pine (Pinus sylvestris L.) and black poplar (Populus nigra L.), were assessed. In the experiment, Ag- NPs were supplied separately to the leaves (via spraying, the foliar treatment) and roots (via watering, the root treatment) of the three species. Uptake, transport and accumulation of Ag were investigated through spectroscopy. The concentration of Ag in the stem was higher in the foliar than in the root treatment, and
in poplar more than in oak and pine. Foliar treatment with Ag-NPs reduced aboveground biomass and stem length in poplars, but not in oaks or pines. Species-specific signals of oxidative stress were observed; foliar treatment of oak caused the accumulation of H2O2 in leaves, and both foliar and root treatments of poplar led to increased O2− in leaves.
Ag-NPs affected leaf and root bacteria and fungi; in the case of leaves, foliar treatment reduced bacterial populations in
oak and poplar and fungi populations in pine, and in the case of roots, root treatment reduced bacteria and increased fungi in poplar. Species-specific mechanisms of interaction, transport, allocation and storage of NPs in trees were found.
We demonstrated definitively that NPs enter into the tree stem through leaves faster than through roots in all of the
investigated tree species.
Ključne besede: Ag-NPs, pathway of uptake, Pinus sylvestris L., Populus nigra L., Quercus pubescens Willd.
Objavljeno v RUNG: 20.04.2020; Ogledov: 2901; Prenosov: 0
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22. A multielement analysis of Cu induced changes in the mineral profilesof Cu sensitive and tolerant populations of Silene paradoxa L.Sara Pignattelli, Ilaria Colzi, Antonella Buccianti, Ilenia Cattani, Gian Maria Beone, Henk Schat, Cristina Gonnelli, 2013, izvirni znanstveni članek Opis: tThis work investigates the Cu induced changes in element profiles in contrasting ecotypes of Silene para-doxa L. A metallicolous copper tolerant population and a non-metallicolous sensitive population weregrown in hydroponics and exposed to different CuSO4treatments. Shoot and root concentrations of Ca,Cu, Fe, K, Mg, Mn, Mo, Na, P, S and Zn were evaluated through ICP-OES.Results indicated that increasing the environmental Cu concentration had a population dependenteffect on element profiles, shoot-to-root ratios and correlations among the elements. Generally, in thetolerant population Cu treatment induced a higher element accumulation in roots and had minimaleffects on the shoot element profile, thus resulting in a progressively decreasing shoot-to-root ratio foreach element. In the sensitive population element concentrations in root and shoot were much moreaffected and without a consistent trend. Copper treatment also affected the correlations between theelements, both in roots and shoots of the two populations, but more so in the sensitive population thanin the tolerant one. Thus, Cu exposure strongly disturbed element homeostasis in the sensitive population,but barely or not in the tolerant one, probably mainly due to a higher capacity to maintain proper rootfunctioning under Cu exposure in the latter. Differences in element profiles were also observed in theabsence of toxic Cu exposure. These differences may reflect divergent population-specific adaptations todifferential nutrient availability levels prevailing in the populations’ natural environments. There is noevidence of inherent side-effects of the Cu tolerance mechanism operating in the tolerant population.
Ključne besede: Mineral profile, Copper tolerance, Silene paradoxa, Compositional data analysis
Objavljeno v RUNG: 20.04.2020; Ogledov: 2857; Prenosov: 0
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23. Quality Control of Purified Proteins to Improve Research Data ReproducibilityStephan Uebel, Ario De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan Knauer, Blanca Lopez-Mendez, Andrea Matagne, Annabel Parret, Kim Remans, Bertrand Raynal, 2019, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Ključne besede: protein quality, biophysical analysis, aggregation
Objavljeno v RUNG: 15.01.2020; Ogledov: 2596; Prenosov: 0
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24. Learning from Nature: Charge Transfer and Carbon Dioxide Activation at Single, Biomimetic Fe Sites in Tetrapyrroles on GrapheneManuel Corva, Fatema Mohamed, Erika Tomsič, Matteo Rinaldi, Cinzia Cepek, Nicola Seriani, Maria Peressi, Erik Vesselli, 2019, izvirni znanstveni članek Ključne besede: carbon dioxide, phthalocyanines, graphene, oxygen, Dirac cones
Objavljeno v RUNG: 03.12.2019; Ogledov: 3236; Prenosov: 0
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25. Substrate- to Laterally-Driven Self-Assembly Steered by Cu Nanoclusters: The Case of FePcs on an Ultrathin Alumina FilmManuel Corva, Fatema Mohamed, Erika Tomsič, Zhijing Feng, Tomas Skala, Giovanni Comelli, Nicola Seriani, Maria Peressi, Erik Vesselli, 2018, izvirni znanstveni članek Ključne besede: copper, nanoclusters, alumina, iron phthalocyanine, Ni3Al
Objavljeno v RUNG: 03.12.2019; Ogledov: 3002; Prenosov: 0
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26. Ectopic calcification in β-thalassemia patients is associated with increased oxidative stress and lower MGP carboxylationFederica Boraldi, Maria Immaculada Garcia Fernandez, Chiara Paolinelli DeVincenzi, Giulia Annovi, Leon J. Schurgers, Cees Vermeer, Paolo Cianciulli, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2013, izvirni znanstveni članek Ključne besede: Elastin
Oxidative stress
Matrix Gla protein
Ectopic calcification
Beta-thalassemia
Objavljeno v RUNG: 23.08.2019; Ogledov: 3193; Prenosov: 0
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27. Fibroblast protein profile analysis highlights the role of oxidative stress and vitamin K recycling in the pathogenesis of pseudoxanthoma elasticum.Federica Boraldi, Giulia Annovi, Deanna Guerra, Paolinelli Devincenzi Chiara, Garcia Fernandez Maria Immaculada, Fulvio Panico, Giorgio De Santis, Roberta Tiozzo, Ivonne Pasquali Ronchetti, Daniela Quaglino, 2009, izvirni znanstveni članek Opis: Pseudoxanthoma elasticum (PXE) is a genetic disorder associated to mutations in the ABCC6 gene; however, the pathogenetic mechanisms leading to elastic fibre calcifications and to clinical manifestations are still unknown. Dermal fibroblasts, directly involved in the production of the extracellular milieu, have been isolated from healthy subjects and from patients affected by PXE, cultured in vitro and characterized for their ability to produce reactive oxygen species, for structural and functional properties of their cell membranes, for changes in their protein profile. Data demonstrate that oxidative stress has profound and endurable consequences on PXE fibroblast phenotype being responsible for: reduced levels of global DNA methylation, increased amount of carbonylated proteins and of lipid peroxidation products, altered structural properties of cell membranes, modified protein expression. Data shed new light on the pathogenetic pathways in PXE, by identifying a network of proteins affecting elastic fibre calcification through inefficient vitamin K recycling, and highlight the role of differentially expressed proteins as targets for validating the efficacy of future therapeutic strategies aiming to delay and/or revert the pathologic phenotype of PXE fibroblasts. Moreover, data open new perspectives for investigating PXE-like phenotypes in the absence of ABCC6 mutations.
Ključne besede: Ectopic calcification / Elastin / Fibroblast proteome / MRP6 / PXE
Objavljeno v RUNG: 23.08.2019; Ogledov: 3413; Prenosov: 0
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28. Use of reactive tracers to determine ambient OH radical concentrations: Application within the indoor environmentIain R. White, Damien Martin, Maria Paz Muñoz, Fredrik K Petersson, Stephen J Henshaw, Graham Nickless, Guy C Lloyd-Jones, Keven C Clemitshaw, Dudley E. Shallcross, 2010, izvirni znanstveni članek Opis: The hydroxyl radical (OH) plays a key role in determining indoor air quality. However, its highly reactive nature and low concentration indoors impede direct analysis. This paper describes the techniques used to indirectly quantify indoor OH, including the development of a new method based on the instantaneous release of chemical tracers into the air. This method was used to detect ambient OH in two indoor seminar rooms following tracer detection by gas chromatography- mass spectrometry (GCMS). The results from these tests add to the small number of experiments that have measured indoor OH which are discussed with regard to future directions within air quality research.
Ključne besede: Ozone, Indoor air pollution, Indoor ozone, chemical tracers
Objavljeno v RUNG: 18.07.2019; Ogledov: 3037; Prenosov: 0
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29. Stimulation of acidic reduction of nitrite to nitric oxide by soybean phenolics: possible relevance to gastrointestinal host defensePablo Ferreira, Maria Souza, Robert Alvin Bernedo Navarro, Rogério Conceição, Edlaine Linhares, Tomomasa Yano, Ione Salgado, 2011, izvirni znanstveni članek Ključne besede: Soybean, nitric oxide, host defense
Objavljeno v RUNG: 27.05.2019; Ogledov: 3026; Prenosov: 0
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30. 17-ß-Estradiol and Testosterone concentrations in claws of puppies up to 60 days of age.Jasmine Fusi, Tanja Peric, Cristina Bergamin, Maria Cristina Veronesi, 2018, objavljeni povzetek znanstvenega prispevka na konferenci Opis: The current 30% of canine perinatal mortality (Tonnessen et al., 2012) claims the need to deepen the knowledge about this phase, characterized by long-term hormonal and metabolic changes, that could benefit of long time-frame methods of investigation, reducing the quantity of samplings and thus respecting animal welfare (Veronesi et al., 2015). Indeed, apart from the well-known invasiveness of blood samplings, the necessary repeated collection of feces, urine and saliva, providing punctual information, could be stressful for the newborns and their mothers with negative effects on health status and maternal behavior.
Given that sexual steroid hormones were reported to have an influence on health outcomes and development already from the perinatal period (Frey et al., 2017), in this work 17-β-Estradiol (E2) and Testosterone (T) concentrations were assessed from the claws of dogs up to 60 days of age, to identify possible endogenous biomarkers.
Ten male and 10 female puppies, viable and healthy, born by elective cesarean section, were enrolled. Samplings were performed by trimming claws at birth, and the regrowth at 30 and 60 days of age after the breeder or the owner signed an informed consent. Then, E2 and T concentrations were analyzed by RIA (Veronesi et al., 2015) and a possible effect of gender evaluated by ANOVA.
All the hormonal claws concentrations showed a significant (p<0.001) drop from birth to 30 and 60 days of age, while no significant changes were observed between 30 and at 60 days of age (Table 1). No influence of the newborns’ gender was found.
Because of the higher levels of E2 and T observed at birth and at 30 days of age, it could be supposed that a source of production could be the placental and maternal compartments. However, the direct involvement of the fetus itself could not be excluded, given the reported accumulation from the nail capillary bed of those hormones (de Berker et al., 2007) and the production of sexual steroid hormones by feline fetal gonads (Braun and Jewgenow, 2017).
Ključne besede: Dog, Puppy, Claws, 17-ß-Estradiol, Testosterone.
Objavljeno v RUNG: 09.04.2019; Ogledov: 3223; Prenosov: 0
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