71. Comparative analysis of fusion tags used to functionalize recombinant antibodiesArio De Marco, Gianluca Veggiani, Barbara Giabbai, Marta Semrau, Barbara Medagli, Vincenzo Riccio, Gregor Bajc, Paola Storici, 2020, izvirni znanstveni članek Ključne besede: recombinant antibodies, protein production, fusion tags Objavljeno v RUNG: 30.09.2019; Ogledov: 3038; Prenosov: 69 Celotno besedilo (740,27 KB) |
72. Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary StudyMarta Montillo, Tanja Peric, 2019, izvirni znanstveni članek Opis: The aim of the study was to perform an investigation on the concentration of 19 minerals and
cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless
sampling, able to represent an integrative values of long-term substance concentrations, and able to
give useful information, also when performed on dead animals, given its extreme stability over time.
In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio
National Park, where official water analysis had pointed out elevated concentrations of As in one of the
two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay),
while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry).
Results showed a negative relationship between cortisol and some mineral concentrations (Li,
Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park
areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance.
This preliminary study represents a step forward in the study of wildlife allostatic load and a valid
method for applications in wildlife management programs, in environmental studies and in public
health programs. Ključne besede: hair, deer, mineral, cortisol Objavljeno v RUNG: 27.08.2019; Ogledov: 3017; Prenosov: 97 Celotno besedilo (2,10 MB) |
73. TD/GC–MS analysis of volatile markers emitted from mono- and co-cultures of Enterobacter cloacae and Pseudomonas aeruginosa in artificial sputumIain R. White, Oluwasola Lawal, Hugo Knobel, Weda Hans, Tamara M E Nijsen, Royston Goodacre, Stephen J. Fowler, Waqar M Ahmed, Antonio Artigas, Jonathan Barnard-Smith, Lieuwe D Bos, Marta Camprubi, Luis Coelho, Paul Dark, Alan Davie, Emili Diaz, Gemma Goma, Timothy Felton, Jan H Leopold, Pouline M P van Oort, Pedro Póvoa, Craig Portsmouth, 2018, izvirni znanstveni članek Opis: Introduction: Infections such as ventilator-associated pneumonia (VAP) can be caused by one or more pathogens. Current methods for identifying these pathogenic microbes often require invasive sampling, and can be time consuming, due to the requirement for prolonged cultural enrichment along with selective and differential plating steps. This results in delays in diagnosis which in such critically ill patients can have potentially life-threatening consequences. Therefore, a non-invasive and timely diagnostic method is required. Detection of microbial volatile organic compounds (VOCs) in exhaled breath is proposed as an alternative method for identifying these pathogens and may distinguish between mono- and poly-microbial infections. Objectives: To investigate volatile metabolites that discriminate between bacterial mono- and co-cultures. Methods: VAP-associated pathogens Enterobacter cloacae and Pseudomonas aeruginosa were cultured individually and together in artificial sputum medium for 24 h and their headspace was analysed for potential discriminatory VOCs by thermal desorption gas chromatography–mass spectrometry. Results: Of the 70 VOCs putatively identified, 23 were found to significantly increase during bacterial culture (i.e. likely to be released during metabolism) and 13 decreased (i.e. likely consumed during metabolism). The other VOCs showed no transformation (similar concentrations observed as in the medium). Bacteria-specific VOCs including 2-methyl-1-propanol, 2-phenylethanol, and 3-methyl-1-butanol were observed in the headspace of axenic cultures of E. cloacae, and methyl 2-ethylhexanoate in the headspace of P. aeruginosa cultures which is novel to this investigation. Previously reported VOCs 1-undecene and pyrrole were also detected. The metabolites 2-methylbutyl acetate and methyl 2-methylbutyrate, which are reported to exhibit antimicrobial activity, were elevated in co-culture only. Conclusion: The observed VOCs were able to differentiate axenic and co-cultures. Validation of these markers in exhaled breath specimens could prove useful for timely pathogen identification and infection type diagnosis. Ključne besede: Bacteria, Enterobacter cloacae, Gas Chromatography-Mass Spectrometry, Infection, Pseudomonas aeruginosa, Volatile organic compounds Objavljeno v RUNG: 18.07.2019; Ogledov: 4544; Prenosov: 114 Celotno besedilo (1,29 MB) |
74. Multi-Messenger Physics With the Pierre Auger ObservatoryKarl-Heinz Kampert, Andrej Filipčič, Gašper Kukec Mezek, Samo Stanič, Marta Trini, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, Lukas Zehrer, 2019, izvirni znanstveni članek Ključne besede: Pierre Auger Observatory, ultra-high energy cosmic rays, photons, neutrinos, protons, multi-messenger physics and astrophysics Objavljeno v RUNG: 06.05.2019; Ogledov: 3363; Prenosov: 107 Celotno besedilo (2,46 MB) |
75. Maternal and fetal hair cortisol concentrations in beef cattle at deliveryTanja Peric, Monica Probo, Antonella Comin, Maria Cristina Veronesi, Marta Montillo, Alberto Prandi, 2018, objavljeni povzetek znanstvenega prispevka na konferenci Opis: Hypothalamus-pituitary-adrenal (HPA) axis of both mother and fetus, is one of key mechanisms involved in fetal programming. Cortisol, the end-product of this axis, plays a critical role in regulating fetal and new-born metabolism and development. The aim of the study was to evaluate maternal and fetal cortisol concentrations shortly after delivery on hair samples non-invasively collected. The study was carried out on 11 pairs of beef cows and their calves (calves were 12 because of one case of twins) from the same farm and subjected to the same managerial conditions. Hair from new-born
calves and from their mothers was
collected on the day of birth shaving their withers to the level of the skin. The hair cortisol concentrations (HCC) were determined by RIA as previously described (Peric et al. 2013: J Dairy Sci 96:3023–3027). The results showed that fetal and maternal HCC ranged from 16.11 to 40.43 pg/mg and from 1.88 to 15.73 pg/mg respectively, with fetal and maternal mean ± SE HCC of 23.72 ± 2.21 and 6.40 ± 1.32 pg/mg respectively (p < 0.001). Fetal HCC were on average 3.5 higher than those of mothers but this ratio differed among the pairs. These differences were also found among the twin calves. These results indicate that in beef cows-and-calves pairs, although HCC at delivery were always higher in calves than in cows, the ratio between fetal and maternal HCC differs among pairs, suggesting an individuality in the fetal programming processes that may also rely on genetic and epigenetic factors. Ključne besede: hair, cortisol, cattle, delivery Objavljeno v RUNG: 09.04.2019; Ogledov: 3552; Prenosov: 0 Gradivo ima več datotek! Več... |
76. Ultra-high energy neutrinos searches with the Pierre Auger ObservatoryMarta Trini, Danilo Zavrtanik, Danilo Zavrtanik, Serguei Vorobiov, Samo Stanič, Gašper Kukec-Mezek, Lukas Zehrer, 2019, objavljeni znanstveni prispevek na konferenci Opis: In the EeV range, neutrinos are expected to be produced by ultra-high energy cosmic rays interactions with the Cosmic Microwave Background during propagation in the Universe. We report on the search for ultra-high energy neutrinos in data collected with the Surface Detector of the Pierre Auger Observatory. The searches are most efficient in the zenith angle range from 60 degrees to 95 degrees with tau neutrinos skimming in the Earth playing dominant role. The present non-detection of UHE neutrinos in the Pierre Auger Observatory excludes the most optimistic scenarios of neutrino production in terms of UHE cosmic rays chemical composition and cosmological evolution of the acceleration sites. We also report on the searches for neutrinos in coincidence with the recent Gravitational Wave events detected by LIGO/Virgo. Ključne besede: EeV neutrinos, Pierre Auger Observatory, limits Objavljeno v RUNG: 26.02.2019; Ogledov: 3830; Prenosov: 0 Gradivo ima več datotek! Več... |
77. All-sky search for correlations in the arrival directions of astrophysical neutrino candidates and ultrahigh-energy cosmic raysI. Al Samarai, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Marta Trini, Darko Veberič, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2017, objavljeni znanstveni prispevek na konferenci Ključne besede: correlations, astrophysical neutrino, ultrahigh-energy cosmic rays Objavljeno v RUNG: 19.02.2018; Ogledov: 3546; Prenosov: 167 Celotno besedilo (3,07 MB) |
78. Testing the agreement between the Xmax distributions measured by the Pierre Auger and Telescope Array ObservatoriesV. De Souza, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Marta Trini, Darko Veberič, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2017, objavljeni znanstveni prispevek na konferenci Ključne besede: Xmax distributions, Pierre Auger Observatory, Telescope Array Objavljeno v RUNG: 19.02.2018; Ogledov: 3826; Prenosov: 179 Celotno besedilo (266,09 KB) |
79. Report of the Telescope Array - Pierre Auger Observatory Working Group on Energy SpectrumDmitri Ivanov, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Darko Veberič, Serguei Vorobiov, Marta Trini, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2017, objavljeni znanstveni prispevek na konferenci Ključne besede: Telescope Array, Pierre Auger Observatory, Energy Spectrum Objavljeno v RUNG: 19.02.2018; Ogledov: 3468; Prenosov: 172 Celotno besedilo (398,98 KB) |
80. Auger at the Telescope Array: toward a direct cross-calibration of surface-detector stationsS. Quinn, Andrej Filipčič, Gašper Kukec Mezek, Ahmed Saleh, Samo Stanič, Marta Trini, Darko Veberič, Serguei Vorobiov, Lili Yang, Danilo Zavrtanik, Marko Zavrtanik, 2017, objavljeni znanstveni prispevek na konferenci Ključne besede: Telescope Array, Pierre Auger Observatory, direct cross-calibration Objavljeno v RUNG: 19.02.2018; Ogledov: 3471; Prenosov: 172 Celotno besedilo (1,76 MB) |