1. Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.Amelie Detterbeck, Paula Pongrac, Daniel Persson, Katarina Vogel-Mikuš, Mitja Kelemen, Primož Vaupetič, Primož Pelicon, Iztok Arčon, Søren Husted, Jan Kofod Shjoerring, Stephan Clemens, 2020, izvirni znanstveni članek Opis: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling
micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations,
localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain
development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively
coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and
sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in
zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations
decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We
hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be
targeted accordingly.
Ključne besede: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE
Objavljeno v RUNG: 20.10.2020; Ogledov: 4219; Prenosov: 0
Gradivo ima več datotek! Več... |
2. Recent advances in 2D imaging of element distribution in Plants by focused beam techniquesKatarina Vogel-Mikuš, Johannes Teun van Elteren, Marjana Regvar, Jitrin Chaiprapa, Boštjan Jenčič, Iztok Arčon, Alojz Kodre, Peter Kump, Anja Kavčič, Mitja Kelemen, Dino Metarapi, Marijan Nečemer, Primož Vavpetič, Primož Pelicon, Paula Pongrac, 2019, samostojni znanstveni sestavek ali poglavje v monografski publikaciji Ključne besede: 2D Imiging, XRF, XANES, synchrotron light
Objavljeno v RUNG: 05.09.2019; Ogledov: 4572; Prenosov: 0
Gradivo ima več datotek! Več... |
3. Cadmium localization and chemical environment in a cadmium bioindicator Gomphrena clausseniiPaula Pongrac, Mina T. Villafort Carvalho, Tânia S. Serra, Hiram Castillo Michel, Katarina Vogel-Mikuš, Iztok Arčon, Luka Jeromel, Mitja Kelemen, Boštjan Jenčič, Anja Kavčič, Primož Pelicon, Mark G. M. Aarts, 2018, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: cadmium
Objavljeno v RUNG: 12.09.2018; Ogledov: 4887; Prenosov: 0
Gradivo ima več datotek! Več... |
4. X-ray spectrometry in plant biologyKatarina Vogel-Mikuš, Iztok Arčon, Alojz Kodre, Anja Kavčič, Peter Kump, Primož Pelicon, 2018, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Opis: Trace elements are essential components of living systems, but at the same time they can be toxic at concentrations beyond those necessary for their biological functions. In addition, the toxicity can be extended to other non-essential elements of very similar atomic characteristics that can mimic the properties of a trace element.
Trace element malnutrition affects more than half of the world’s population, while on the other hand industrialization, traffic and extensive use of fertilizers have resulted in exceedingly high concentrations of non-essential elements in food crops, posing risks to human health.
In order to be able to develop and improve phyto-technologies that enable production of safe and quality food, knowledge on the basic mechanisms involved in trace and non-essential element uptake, transport, accumulation and ligand environment in plants is needed.
Such studies are nowadays supported by highly sophisticated X-ray based techniques, such as synchrotron based X-ray fluorescence spectrometry, proton induced X-ray emission and X-ray absorption spectroscopy, enabling imaging of element distribution and determination of speciation and ligand environment of trace elements in biological tissues and cells with high spatial resolution and sensitivity.
Selected case studies of metal distribution and speciation in selected model and crop plants, achieved by interdisciplinary work, will be presented.
Ključne besede: X-ray spectrometry, plants, XANES, EXAFS
Objavljeno v RUNG: 12.09.2018; Ogledov: 4735; Prenosov: 0
Gradivo ima več datotek! Več... |
5. |
