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2. Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.Amelie Detterbeck, Paula Pongrac, Daniel Persson, Iztok Arčon, Primož Pelicon, Primož Vaupetič, Mitja Kelemen, Søren Husted, Katarina Vogel-Mikuš, Stephan Clemens, Jan Kofod Shjoerring, 2020, izvirni znanstveni članek Opis: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling
micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations,
localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain
development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively
coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and
sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in
zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations
decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We
hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be
targeted accordingly.
Najdeno v: osebi
Ključne besede: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE
Objavljeno: 20.10.2020; Ogledov: 1936; Prenosov: 0
 Polno besedilo (4,76 MB) |
3. X-ray spectrometry in plant biologyPrimož Pelicon, Peter Kump, Anja Kavčič, Alojz Kodre, Iztok Arčon, Katarina Vogel-Mikuš, 2018, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Opis: Trace elements are essential components of living systems, but at the same time they can be toxic at concentrations beyond those necessary for their biological functions. In addition, the toxicity can be extended to other non-essential elements of very similar atomic characteristics that can mimic the properties of a trace element.
Trace element malnutrition affects more than half of the world’s population, while on the other hand industrialization, traffic and extensive use of fertilizers have resulted in exceedingly high concentrations of non-essential elements in food crops, posing risks to human health.
In order to be able to develop and improve phyto-technologies that enable production of safe and quality food, knowledge on the basic mechanisms involved in trace and non-essential element uptake, transport, accumulation and ligand environment in plants is needed.
Such studies are nowadays supported by highly sophisticated X-ray based techniques, such as synchrotron based X-ray fluorescence spectrometry, proton induced X-ray emission and X-ray absorption spectroscopy, enabling imaging of element distribution and determination of speciation and ligand environment of trace elements in biological tissues and cells with high spatial resolution and sensitivity.
Selected case studies of metal distribution and speciation in selected model and crop plants, achieved by interdisciplinary work, will be presented.
Najdeno v: osebi
Ključne besede: X-ray spectrometry, plants, XANES, EXAFS
Objavljeno: 12.09.2018; Ogledov: 2762; Prenosov: 0
Polno besedilo (62,44 KB) |
4. Cadmium localization and chemical environment in a cadmium bioindicator Gomphrena clausseniiMark G. M. Aarts, Primož Pelicon, Anja Kavčič, Boštjan Jenčič, Mitja Kelemen, Luka Jeromel, Iztok Arčon, Katarina Vogel-Mikuš, Hiram Castillo Michel, Tânia S. Serra, Mina T. Villafort Carvalho, Paula Pongrac, 2018, objavljeni povzetek znanstvenega prispevka na konferenci Najdeno v: osebi
Ključne besede: cadmium
Objavljeno: 12.09.2018; Ogledov: 2815; Prenosov: 0
Polno besedilo (117,55 KB) |
5. Recent advances in 2D imaging of element distribution in Plants by focused beam techniquesKatarina Vogel-Mikuš, Johannes Teun van Elteren, Marjana Regvar, Jitrin Chaiprapa, Boštjan Jenčič, Iztok Arčon, Alojz Kodre, Peter Kump, Anja Kavčič, Mitja Kelemen, Dino Metarapi, Marijan Nečemer, Primož Vavpetič, Primož Pelicon, Paula Pongrac, 2019, samostojni znanstveni sestavek ali poglavje v monografski publikaciji Najdeno v: osebi
Ključne besede: 2D Imiging, XRF, XANES, synchrotron light
Objavljeno: 05.09.2019; Ogledov: 2402; Prenosov: 0
Polno besedilo (8,41 MB) |
