1. Protein quality control for improved data reproducibility and reliabilityKim Remans, Ario De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan Knauer, Andre Matagne, Anne Perret, Stephan Uebel, Bertrand Raynal, 2022, objavljeni povzetek znanstvenega prispevka na konferenci Ključne besede: protein quality, evaluation assays, biophysical methods Objavljeno v RUNG: 13.04.2023; Ogledov: 769; Prenosov: 0 Gradivo ima več datotek! Več... |
2. Quality control of protein reagents for the improvement of research data reproducibilityArio De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, kratki znanstveni prispevek Ključne besede: protein quality, protein aggregation, data reproducibility Objavljeno v RUNG: 17.05.2021; Ogledov: 2002; Prenosov: 13 Povezava na celotno besedilo Gradivo ima več datotek! Več... |
3. Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale surveyNick Berrow, Ario De Marco, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, izvirni znanstveni članek Ključne besede: protein quality, biophysical analysis, protein aggregates Objavljeno v RUNG: 22.04.2021; Ogledov: 2012; Prenosov: 0 Gradivo ima več datotek! Več... |
4. Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.Amelie Detterbeck, Paula Pongrac, Daniel Persson, Katarina Vogel-Mikuš, Mitja Kelemen, Primož Vaupetič, Primož Pelicon, Iztok Arčon, Søren Husted, Jan Kofod Shjoerring, Stephan Clemens, 2020, izvirni znanstveni članek Opis: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling
micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations,
localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain
development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively
coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and
sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in
zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations
decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We
hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be
targeted accordingly. Ključne besede: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE Objavljeno v RUNG: 20.10.2020; Ogledov: 2669; Prenosov: 0 Gradivo ima več datotek! Več... |
5. Arabidopsis halleri shows hyperbioindicator behaviour for Pb and leaf Pb accumulation spatially separated from ZnStephan Höreth, Paula Pongrac, Marta Debeljak, Katarina Vogel-Mikuš, Matic Pečovnik, Primož Vavpetič, Iztok Arčon, izvirni znanstveni članek Opis: Lead (Pb) ranks among the most problematic environmental pollutants. Background contamination of soils is nearly ubiquitous, yet plant Pb accumulation is barely understood. In a survey covering 165 European populations of the metallophyte Arabidopsis halleri, several field samples had indicated Pb hyperaccumulation, offering a chance to dissect plant Pb accumulation.
Accumulation of Pb was analysed in A. halleri individuals from contrasting habitats under controlled conditions to rule out aerial deposition as a source of apparent Pb accumulation. Several elemental imaging techniques were employed to study the spatial distribution and ligand environment of Pb.
Regardless of genetic background, A. halleri individuals showed higher shoot Pb accumulation than A. thaliana. However, dose–response curves revealed indicator rather than hyperaccumulator behaviour. Xylem sap data and elemental imaging unequivocally demonstrated the in planta mobility of Pb. Highest Pb concentrations were found in epidermal and vascular tissues. Distribution of Pb was distinct from that of the hyperaccumulated metal zinc. Most Pb was bound by oxygen ligands in bidentate coordination.
A. halleri accumulates Pb whenever soil conditions render Pb phytoavailable. Considerable Pb accumulation under such circumstances, even in leaves of A. thaliana, strongly suggests that Pb can enter food webs and may pose a food safety risk. Ključne besede: Pb accumulation, XANES, EXAFS, Arabidopsis halleri Objavljeno v RUNG: 16.01.2020; Ogledov: 2969; Prenosov: 0 Gradivo ima več datotek! Več... |
6. Quality Control of Purified Proteins to Improve Research Data ReproducibilityStephan Uebel, Ario De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan Knauer, Blanca Lopez-Mendez, Andrea Matagne, Annabel Parret, Kim Remans, Bertrand Raynal, 2019, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Ključne besede: protein quality, biophysical analysis, aggregation Objavljeno v RUNG: 15.01.2020; Ogledov: 2505; Prenosov: 0 Gradivo ima več datotek! Več... |