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Fluorescent covalent organic frameworks : promising bioimaging materials
Chimatahalli Santhakumar Karthik, Tina Škorjanc, Dinesh Shetty, 2024, izvirni znanstveni članek

Opis: Fluorescent covalent organic frameworks (COFs) have emerged as promising candidates for imaging living cells due to their unique properties and adjustable fluorescence. In this mini-review, we provide an overview of recent advancements in fluorescent COFs for bioimaging applications. We discuss the strategies used to design COFs with desirable properties such as high photostability, excellent biocompatibility, and pH sensitivity. Additionally, we explore the various ways in which fluorescent COFs are utilized in bioimaging, including cellular imaging, targeting specific organelles, and tracking biomolecules. We delve into their applications in sensing intracellular pH, reactive oxygen species (ROS), and specific biomarkers. Furthermore, we examine how functionalization techniques enhance the targeting and imaging capabilities of fluorescent COFs. Finally, we discuss the challenges and prospects in the field of fluorescent COFs for bioimaging in living cells, urging further research in this exciting area.
Ključne besede: covalent organic frameworks, fluorescent materials, imaging, bioimaging, biosensors
Objavljeno v RUNG: 05.03.2024; Ogledov: 607; Prenosov: 3
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Biological applications of porous polymers and covalent organic frameworks : lecture at the Otto-von-Guericke Universität Magdeburg, Tuesday, 27th June 2023, Magdeburg, Germany
Tina Škorjanc, 2023, prispevek na konferenci brez natisa

Opis: Porous organic polymers (POPs) and covalent organic frameworks (COFs) have gained significant attention in the scientific community for a wide array of applications because of their attractive physical and chemical properties. Porosity of these materials provides ample surface area for interaction with targets, while crystallinity allows for highly specific structural tuning. In this seminar, I will present two strategies of utilizing these features of newly prepared materials in biosensing. Firstly, a cationic POP was synthesized, deposited onto interdigitated electrode arrays via a nontraditional electrophoresis technique, and utilized for electrochemical sensing of bacterial cells. As the principle of detection relied on electrostatic interactions between the cationic POP and the anionic bacterial surface, the sensor operated for both Gram-positive and Gram-negative bacteria. Secondly, a small-molecule nitroimidazole target for hypoxia, a low oxygen environment present in tumors, was post-synthetically conjugated to the pores of a fluorescent COF. This material served as a useful hypoxia imaging agent in cancerous cells. The seminar will conclude with some future perspectives on POPs and COFs in biological applications followed by Q & A.
Ključne besede: Porous organic polymers, covalent organic frameworks, biosensors, hypoxia
Objavljeno v RUNG: 13.07.2023; Ogledov: 1060; Prenosov: 0
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Recombinant ligands as tunable biotechnological tools
Ario De Marco, predavanje na tuji univerzi

Ključne besede: nanobodies, protein functionalization, biosensors
Objavljeno v RUNG: 17.05.2022; Ogledov: 1650; Prenosov: 0
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Optimised calibration procedure for bioanalytical determination of organophosphate pesticides in apple juices by immobilised AChE
Boris Pihlar, Klavdija Mežnar, Mladen Franko, Lea Pogačnik, 2003, izvirni znanstveni članek

Opis: Optimised calibration procedure for bioanalytical determination of organophosphate pesticides in apple juices by immobilised AChE
Ključne besede: analitske metode, biosenzorji, pesticidi, sadni sokovi, analytical techniques, biosensors, FIA, flow injection analysis, pesticides, fruit juices
Objavljeno v RUNG: 17.05.2016; Ogledov: 5062; Prenosov: 17
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