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2. Določanje nizkih koncentracij acetamiprida v ekotoksikoloških matriksih ter ugotavljanje njegove strupenosti za kopenske enakonožne rake vrste Porcellio scaber (Isopoda, Crustacea)Ana Kete, 2007, diplomsko delo Najdeno v: ključnih besedah
Povzetek najdenega: ...diplomske naloge, acetamiprid, Porcellio scaber, prehranjevalni parametri, HPLC-TLS, ...
Ključne besede: diplomske naloge, acetamiprid, Porcellio scaber, prehranjevalni parametri, HPLC-TLS
Objavljeno: 15.10.2013; Ogledov: 5784; Prenosov: 361
 Polno besedilo (0,00 KB)
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8. Identification of sweet cherry anthocyanins and hydroxycinnamic acids using HPLC coupled with DAD and MS detectorPolonca Trebše, Branka Mozetič Vodopivec, 2004, izvirni znanstveni članek Opis: In absence of standards, HPLC coupled with DAD offers identification of polyphenols by scanning UV-Vis spectra of individual components, which spectral characteristics are unique, but not selective. At the same time HPLC-DAD determination methods of polyphenols differ in mobile phase solutions resulting in DAD scanned spectra deviation between different studies, aggravating the precise identification based on agreement to UV-Vis data from literature. Mass spectrometry (MS) detection with molar weight determination of the individual components in the sample enables more precise identification of compounds eluted from the column. Sweet cherry Petrovka polyphenols were separated on C18 Hypersil PEP 300 column (250 x 4.6 mm, 5 [mu]m) using gradient solvent mixture consisting of methanol, water and formic acid. MS and UV-Vis spectra of eluted anthocyanins and hydroxycinnamic acid were recorded. HPLC-MS analyses were performed using a LCQ [sup]TM ion trap, Finnigan, MAT mass spectrometer by atmospheric pressure chemical ionisation (ACPI). Molecular and fragmented ion masses of sweet cherry hydroxycinnamic acids and anthocyanins were determined and with UV-Vis spectra, in the range of 190-600 nm, used for identification of compounds. Electro spray mass spectrum of two hydroxycinnamic acids produced ions with m/z ratios of 353.0 and 337.0, which corresponded to molecular weights of neochlorogenic acid and 3'-p-coumarylquinic acid. The molecular weights of 5 anthocyanins corresponded to cyanidin-3-glucoside (449.0), cyanidin-3-rutinoside (595.1), peonidin-3-glucoside (463.1), pelargonidin-3-rutinoside (579.1) and peonidin-3-rlltinoside (609.2).
Najdeno v: ključnih besedah
Ključne besede: češnje, antociani, hidroksicimetne kisline, HPLC-DAD/MS
Objavljeno: 10.07.2015; Ogledov: 5113; Prenosov: 18
Polno besedilo (0,00 KB) |
9. Hydroxyl radical scavenging-based method for evaluation of TiO[sub]2 photocatalytic activityUrška Lavrenčič Štangar, Matija Strlič, Romana Cerc Korošec, Danijela Pucko, 2013, izvirni znanstveni članek Opis: Hydroxyl radical scavenging-based method for evaluation of TiO[sub]2 photocatalytic activity
Najdeno v: ključnih besedah
Ključne besede: titanium dioxide, photocatalysis, N, N-(5-nitro-1, 3-phenylene)bisglutaramide, radical scavenger, HPLC
Objavljeno: 05.01.2016; Ogledov: 4410; Prenosov: 28
Polno besedilo (0,00 KB) |
10. Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serumMitja Martelanc, Lovro Žiberna, Sabina Passamonti, Mladen Franko, 2016, izvirni znanstveni članek Opis: We present the applicability of a new ultra sensitive analytical method for the simultaneous determination of biliverdin and bilirubin in human serum. The method comprises isocratic reversed-phase(RP) C18 high-performance liquid chromatography (HPLC) and thermal lens spectrometric detection (TLS)based on excitation by a krypton laser emission line at 407 nm. This method enables the separation of IX-α biliverdin and IX-α bilirubin in 11 min. with limit of detection (LOD) and limit of quantitation (LOQ) for
biliverdin of 1.2 nM and 3 nM, and 1nM and 2.8 nM for bilirubin,respectively.In addition, a step-gradient elution was set up, by changing the mobile phase composition, in order to further enhance the sensitivity for bilirubin determination with LOD and LOQ of 0.5 nM and 1.5 nM, respectively. In parallel, an isocraticHPLC-DAD method was developed for benchmarking against HPLC-TLS methods. The LOD
and LOQ forbiliverdin were 6 nM and 18 nM, and 2.5 nM and 8nM for bilirubin,respectively. Additionally, both isocratic methods were applied for measuring biliverdin and free bilirubin in human serum samples (from 2 male and 2 female healthy donors). Combining isocratic HPLC method with TLS
detector was crucial for first ever biliverdin determination in serum together with simultaneous free bilirubin determination. We showed for the first time the concentration ratio of free bilirubin versus unbound biliverdin in human serum samples.
Najdeno v: ključnih besedah
Povzetek najdenega: ...isocraticHPLC-DAD method was developed for benchmarking against HPLC-TLS methods. The LOD
and LOQ forbiliverdin were 6...
Ključne besede: Bilirubin, biliverdin, serum, HPLC, TLS
Objavljeno: 29.03.2016; Ogledov: 4765; Prenosov: 0
 Polno besedilo (964,28 KB) |
