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Isolation of adhirons specific for plant protoplast membrane biomarkers is simplified by phagemid design
Claudia D'Ercole, Ario De Marco, 2023, samostojni znanstveni sestavek ali poglavje v monografski publikaciji

Ključne besede: pea protoplasts, ALFAtag, customized reagents, adhirons, protoplast biomarkers
Objavljeno v RUNG: 19.07.2023; Ogledov: 659; Prenosov: 1
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Diagnostics and Monitoring of COVID-19 Infection – Current Understanding
Martina Bergant Marušič, Ario De Marco, 2020, recenzija, prikaz knjige, kritika

Ključne besede: SARS-CoV-2, RT-PCR, seroconversion, serum biomarkers, neutralizing antibodies
Objavljeno v RUNG: 30.09.2022; Ogledov: 1124; Prenosov: 20
.pdf Celotno besedilo (870,49 KB)

Identification of environmental stress conditions by comparative analysis of peroxidase activity in betulla pendula and pinus sp. : Group project report
Patrik Cingerli, Katarina Erker, Nina Žvab-Pernat, Darian Rampih, Miroslav Štrbac, Hena Zukić, Kenan Kapetanović, 2021, elaborat, predštudija, študija

Opis: The study investigated the possibility of developing a biomarker to evaluate the stress level in trees. The analyses have been performed on tree samples appertaining to two different tree species and collected at two locations in Slovenia. The presence and the activity of the proteins – peroxidases was measured by the qualitative method of the agarose gel electrophoresis and the quantitative method of spectrometry. It was found out that by measuring the presence of the biomarkers and pigments in the tree samples we can determine the stress levels and thus the health status of trees.
Ključne besede: biomarkers, environmental stress, pigments, peroxidase
Objavljeno v RUNG: 29.09.2021; Ogledov: 2006; Prenosov: 0
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Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness
Sandra Oloketuyi, Giulia Annovi, Ario De Marco, 2020, izvirni znanstveni članek

Ključne besede: zymograms, peroxidase, agarose gel, forest tree, biochemical biomarkers
Objavljeno v RUNG: 22.04.2020; Ogledov: 2835; Prenosov: 0
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Exhaled breath metabolomics reveals a pathogen-specific response in a rat pneumonia model for two human pathogenic bacteria: a proof-of-concept study
Pouline M van Oort, Iain R. White, 2019, izvirni znanstveni članek

Opis: Volatile organic compounds in breath can reflect host and pathogen metabolism and might be used to diagnose pneumonia. We hypothesized that rats with Streptococcus pneumoniae (SP) or Pseudomonas aeruginosa (PA) pneumonia can be discriminated from uninfected controls by thermal desorption-gas chromatography-mass-spectrometry (TD-GC-MS) and selected ion flow tube-mass spectrometry (SIFT-MS) of exhaled breath. Male adult rats (n = 50) received an intratracheal inoculation of 1) 200 µl saline, or 2) 1 × 107 colony-forming units of SP or 3) 1 × 107 CFU of PA. Twenty-four hours later the rats were anaesthetized, tracheotomized, and mechanically ventilated. Exhaled breath was analyzed via TD-GC-MS and SIFT-MS. Area under the receiver operating characteristic curves (AUROCCs) and correct classification rate (CCRs) were calculated after leave-one-out cross-validation of sparse partial least squares-discriminant analysis. Analysis of GC-MS data showed an AUROCC (95% confidence interval) of 0.85 (0.73-0.96) and CCR of 94.6% for infected versus noninfected animals, AUROCC of 0.98 (0.94-1) and CCR of 99.9% for SP versus PA, 0.92 (0.83-1.00), CCR of 98.1% for SP versus controls and 0.97 (0.92-1.00), and CCR of 99.9% for PA versus controls. For these comparisons the SIFT-MS data showed AUROCCs of 0.54, 0.89, 0.63, and 0.79, respectively. Exhaled breath analysis discriminated between respiratory infection and no infection but with even better accuracy between specific pathogens. Future clinical studies should not only focus on the presence of respiratory infection but also on the discrimination between specific pathogens.
Ključne besede: biomarkers, exhaled breath analysis, infection, pneumonia
Objavljeno v RUNG: 22.07.2019; Ogledov: 3038; Prenosov: 0
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Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers
Pieranna Chiarella, Renata Sisto, Ario De Marco, 2018, izvirni znanstveni članek

Ključne besede: biomarkers, gene polymorphism, NRF2, oxidative stress, PCR, RFLP
Objavljeno v RUNG: 11.12.2018; Ogledov: 3893; Prenosov: 141
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Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy
Ario De Marco, Milica Popovic, 2017, pregledni znanstveni članek

Ključne besede: Extracellular vesicles, exosome biomarkers, immune-purification
Objavljeno v RUNG: 25.09.2017; Ogledov: 3966; Prenosov: 0
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Nanobodies as biotechnological tools
Ario De Marco, predavanje na tuji univerzi

Ključne besede: nanobodies, pre-immune phage library, recombinant expression, biomarkers, tumors
Objavljeno v RUNG: 17.05.2017; Ogledov: 3676; Prenosov: 0
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