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The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
Boraldi Federica, Annovi Giulia, Paolinelli Devincenzi Chiara, Tiozzo Roberta, Quaglino Daniela, 2008, izvirni znanstveni članek

Opis: The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to significantly change their expression, the great majority of protein changes were related to the cytoskeleton, the stress response and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell culture can adapt themselves to environmental changes, without significantly altering cell viability, at least after a few days of treatment, even though serum withdrawal represents a stress condition capable to increase ROS production, to influence cell metabolism and to interfere with cell behaviour, favouring the expression of several age-related features.
Ključne besede: Dermal fibroblasts / Primary cell culture / ROS production / Serum withdrawal
Objavljeno v RUNG: 22.07.2019; Ogledov: 3236; Prenosov: 0
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13.
Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach.
Boraldi Federica, Annovi Giulia, Carraro Fabio, Naldini Antonella, Tiozzo Roberta, Sommer Pascal, Quaglino Daniela, 2007, izvirni znanstveni članek

Opis: The ability of cells to respond to changes in oxygen availability is critical for many physiological and pathological processes (i.e. development, aging, wound healing, hypertension, cancer). Changes in the protein profile of normal human dermal fibroblasts were investigated in vitro after 96 h in 5% CO2 and 21% O2 (pO2=140 mm Hg) or 2% O2 (pO2=14 mm Hg), these parameters representing a mild chronic hypoxic exposure which fibroblasts may undergo in vivo. The proliferation rate and the protein content were not significantly modified by hypoxia, whereas proteome analysis demonstrated changes in the expression of 56 proteins. Protein identification was performed by mass spectrometry. Data demonstrate that human fibroblasts respond to mild hypoxia increasing the expression of hypoxia inducible factor (HIF1a) and of the 150-kDa oxygen-regulated protein. Other differentially expressed proteins appeared to be related to stress response, transcriptional control, metabolism, cytoskeleton, matrix remodelling and angiogenesis. Furthermore, some of them, like galectin 1, 40S ribosomal protein SA, N-myc-downstream regulated gene-1 protein, that have been described in the literature as possible cancer markers, significantly changed their expression also in normal hypoxic fibroblasts. Interestingly, a bovine fetuin was also identified that appeared significantly less internalised by hypoxic fibroblasts. In conclusion, results indicate that human dermal fibroblasts respond to an in vitro mild chronic hypoxic exposure by modifying a number of multifunctional proteins. Furthermore, data highlight the importance of stromal cells in modulating the intercellular cross-talk occurring in physiological and in pathologic conditions.
Ključne besede: Human fibroblast, Primary cell culture, Hypoxia, Connective tissue, Proteome, 2D gel electrophoresis, Mass-spectrometry
Objavljeno v RUNG: 22.07.2019; Ogledov: 3288; Prenosov: 0
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14.
ACCESSIBILITY AS A NON-PREJUDICIAL APPROACH: IS CULTURE BACK TO NORMALITY? : Exploring the emotional narrative of interactive andimmersive exhibitions.
Ilaria Bollati, 2018, doktorska disertacija

Opis: This research explores the general and actual accessibility to cultural spaces and organizations, focusing on the network of relationships between contents, innovation, and participation. It investigates how Culture can be perceived as a normal experience, actually able to set a rich dialogue with each of us, normal ordinary people/consumers. Normality means inclusion and sharing. The proposed investigation is based on a triad of interactions among culture, economics, and design. Assuming that Culture generates its value from a cognitive approach, or from a dynamic and context-dependent value chain that is subject to a cognitive evolution, this research acknowledges that the cultural experience is subject to a double issue of access: - The horizontal question is related to complications associated with the structure. Believing that ‘culture is special’ implies the risk of progressively widening the gap between cultural supply and society. Culture is ‘locked’ in specific sites and a big slice of the world’s adult population has yet to be involved in any cultural experience. - Once physically inside the cultural structure, the vertical issue is generated by the difficulty in entering in contact with the offered contents. This research focuses only on museums and multimedia exhibitions in which the learning process has changed: the research presents itself as a conversation where both ‘those who know’ and ‘those who learn’ play equally active parts in a relative process of understanding. Starting from the existing processes, forms, previous studies and case studies, the survey yearns for their systematization in innovative models. The process, from theory to practice and vice versa, goes beyond a traditional mechanism of deduction: it moves from specific contexts to the abstraction of replicable approaches. The question of how the narration emerges guides you toward a new method of analysis, study, and cataloguing; a schematization capable of investigating not only knowledge, but also the visitor's ‘cognitive metabolism’ (how knowledge is acquired) during the multimedia experience; an innovative multipurpose tool, useful for both the museum institution and the designer. The research finally assesses and takes into account an actual experiment, the outcomes of which may prove to be useful in feeding theoretical implications with empirical experiences: RovelloDue - Piccolo Spazio Politecnico, a temporary multimedia space.
Ključne besede: audience development, cognitive accessibility, cultural addiction, culture, human, emotion, immersion, innovation, interactive exhibitions, participation, natural interfaces, normality, technologies
Objavljeno v RUNG: 13.12.2018; Ogledov: 3465; Prenosov: 61
.pdf Celotno besedilo (45,89 MB)

15.
Извештај од симпозиумот "Улогата на астрономијата во општеството и културата"
Tanja Petrushevska, 2009, poljudni članek

Opis: По завршувањето на церемонијата на отворањето на Меѓународната година на астрономијата (МГА), за која читавте во претходниот број на ЕМИТЕР, во просториите на УНЕСКО во Париз од 19 до 23 јануари беше одржан Симпозиум за улогата на астрономијата во општеството и културата. Симпозиумот беше организиран од страна на Меѓународната астрономска унија (International Astronomy Union, IAU) во рамките на настани за одбележување на МГА. На овој симпозиум учествуваше и македонска делегација.
Ključne besede: report from the international symposium "The role of astronomy in society and culture! organized by the International Astronomy Union
Objavljeno v RUNG: 29.01.2018; Ogledov: 3177; Prenosov: 0
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16.
An Artist Abroad : The influence of a change in surroundings on artistic creativity
Valerija Zabret, 2016, magistrsko delo

Opis: The motivation for this thesis was author’s experience as a traveling artists and questioning how experiences abroad influenced her artistic evolution. Nowadays, in the years of globalization and cross-border collaborations, it has become very common for artists to make international connections in order to seek inspiration, funds, connections, exhibition spaces, platforms on which to show their work, studios, workshops, and to establish an international career. The idea of this thesis is to explore the phenomena of traveling artists through literature and analysis of the author’s personal experience and development as an artist in order to identify whether travel is a good practice that should be advised for emerging (or established) artists in order to evolve in their careers. This thesis, “The Influence of a Change in Surroundings on Artistic Creativity,” questions how traveling influences artists and their creativity. It offers a historically overview of the subject and presents contemporary ways and opportunities for artists to travel and work in international environments. In the second part of this thesis, the author presents poetical videos, the “An Artist Abroad – Video Poetry Series,” which were made during the research and through which the author expresses her feelings and observation of her new surroundings. Very personal and poetic work shows the progress and development in the author’s artistic practice, and it shows how new surroundings influenced her creative and production processes.
Ključne besede: art, artist, artist abroad, artist residency, traveling artist, mobility, surroundings, change, creativity, influence, production, culture, global, contemporary art, video, poetry, series
Objavljeno v RUNG: 25.02.2016; Ogledov: 6557; Prenosov: 159
.pdf Celotno besedilo (7,21 MB)
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