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Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples
Mingqiang Liu, Sara Malovrh, Mladen Franko, 2016, izvirni znanstveni članek

Opis: An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums.
Ključne besede: Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI)
Objavljeno v RUNG: 01.06.2016; Ogledov: 5486; Prenosov: 273
.pdf Celotno besedilo (828,33 KB)

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Sorpcija kroma iz vodnih raztopin na naravni in sintetični zeolit
Saša Rozman, 2009, diplomsko delo

Ključne besede: diplomske naloge, čiščenje odpadnih voda, zeolit, krom
Objavljeno v RUNG: 15.10.2013; Ogledov: 7086; Prenosov: 473
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