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2. Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertationGbenga Folorunsho Oginni, 2024, doktorska disertacija Opis: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Ključne besede: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Objavljeno v RUNG: 10.09.2024; Ogledov: 404; Prenosov: 5
 Celotno besedilo (3,80 MB) |
3. Urinary extracellular vesicles as a readily available biomarker source : a simplified stratification methodLidija Filipović, Milica Spasojević Savković, Radivoje Prodanović, Suzana Matijašević Joković, Sanja Stevanović, Ario De Marco, Maja Kosanović, Goran Brajušković, Milica Popovic, 2024, izvirni znanstveni članek Ključne besede: nanobodies, extracellular vesicles, immunoaffinity purification
Objavljeno v RUNG: 23.07.2024; Ogledov: 711; Prenosov: 5
Celotno besedilo (3,55 MB)
Gradivo ima več datotek! Več... |
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5. Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF-▫$\alpha$▫ and IL-23p19 bioactivitiesJiewen Wang, Guangbo Kang, Huiying Lu, Ario De Marco, Haibin Yuan, Zelin Feng, Mengxue Gao, Xiaoli Wang, Huahong Wang, Xiaolan Zhang, 2024, izvirni znanstveni članek Ključne besede: bispecific constructs, nanobodies, intestinal inflammation
Objavljeno v RUNG: 27.03.2024; Ogledov: 1313; Prenosov: 5
Celotno besedilo (12,21 MB)
Gradivo ima več datotek! Več... |
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