1. Design of nanobody-based bispecific constructs by in silico affinity maturation and umbrella sampling simulationsZixuan Bai, Jiewen Wang, Jiaqi Li, Haibin Yuan, Ping Wang, Miao Zhang, Yuanhang Feng, Xiangtong Cao, Xiangan Cao, Guangbo Kang, Ario de Marco, He Huang, 2023, izvirni znanstveni članek Ključne besede: nanobody rational design, bispecific binders, protein activity inhibition
Objavljeno v RUNG: 03.01.2023; Ogledov: 542; Prenosov: 0
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2. Research progress and applications of multivalent, multispecific and modified nanobodies for disease treatmentJiewen Wang, Guangbo Kang, Haibin Yuan, Xiaocang Cao, He Huang, Ario De Marco, 2022, izvirni znanstveni članek Ključne besede: nanobody multimers, immunomodulation, intrabodies, imaging, nanobody functionalization
Objavljeno v RUNG: 18.01.2022; Ogledov: 1270; Prenosov: 149
 Povezava na celotno besedilo
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3. Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable RegionMiguel Soler, Barbara Medagli, Jiewen Wang, Sandra Oloketuyi, Gregor Bajc, He Huang, Sara Fortuna, Ario de Marco, 2021, izvirni znanstveni članek Ključne besede: nanobody framework, modeling, nanobody humanization, CDR grafting
Objavljeno v RUNG: 27.01.2021; Ogledov: 1931; Prenosov: 60
 Celotno besedilo (2,00 MB) |
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5. Identification of New DR5 Agonistic Nanobodies and Generation of Multivalent Nanobody Constructs for Cancer TreatmentGolnaz Sadeghnezhad, Ema Romao, Robert Alvin Bernedo-Navarro, Sam Massa, Khosro Khajeh, Serge Muyldermans, Sadegh Hassania, 2019, izvirni znanstveni članek Ključne besede: death receptor 5, nanobody, TRAIL, cancer therapy, apoptosis
Objavljeno v RUNG: 01.10.2019; Ogledov: 2551; Prenosov: 0
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6. Nanobodies: towards rational design of immune-reagentsArio De Marco, 2017, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje) Opis: Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Ključne besede: recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Objavljeno v RUNG: 21.03.2018; Ogledov: 3948; Prenosov: 0
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