Repozitorij Univerze v Novi Gorici

Iskanje po repozitoriju
A+ | A- | Pomoč | SLO | ENG

Iskalni niz: išči po
išči po
išči po
išči po
* po starem in bolonjskem študiju

Opcije:
  Ponastavi


1 - 10 / 16
Na začetekNa prejšnjo stran12Na naslednjo stranNa konec
1.
2.
Combining panning design and expression options allows obtaining nanobodies trimmed for specific applications
Ario De Marco, predavanje na tuji univerzi

Ključne besede: nanobodies, panning, functionalization
Objavljeno v RUNG: 15.05.2023; Ogledov: 1024; Prenosov: 0
Gradivo ima več datotek! Več...

3.
Nanobodies isolated from pre-immune libraries as tunable biotechnological tools
Ario De Marco, predavanje na tuji univerzi

Ključne besede: nanobodies, panning, modeling
Objavljeno v RUNG: 27.02.2020; Ogledov: 2533; Prenosov: 0
Gradivo ima več datotek! Več...

4.
Pre-immune libraries of recombinant nanobodies: outcome from 15-year experience
Ario De Marco, predavanje na tuji univerzi

Ključne besede: Nanobodies, in vitro panning, protein functionalization
Objavljeno v RUNG: 14.02.2020; Ogledov: 2881; Prenosov: 0
Gradivo ima več datotek! Več...

5.
Nanobodies: How to Exploit Pre-Immune Libraries and Recombinant Antibody Fragments
Ario De Marco, vabljeno predavanje na konferenci brez natisa

Ključne besede: nanobodies, in vitro panning, phage display
Objavljeno v RUNG: 23.08.2019; Ogledov: 3235; Prenosov: 0
Gradivo ima več datotek! Več...

6.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, predavanje na tuji univerzi

Ključne besede: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Objavljeno v RUNG: 21.09.2018; Ogledov: 3378; Prenosov: 0
Gradivo ima več datotek! Več...

7.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, predavanje na tuji univerzi

Ključne besede: phage libraries, nanobodies, in vitro antibody panning
Objavljeno v RUNG: 17.09.2018; Ogledov: 3216; Prenosov: 0
Gradivo ima več datotek! Več...

8.
Nanobodies: a flexible technological platform
Ario De Marco, predavanje na tuji univerzi

Ključne besede: Nanobodies, immunoreagents, recombinant antibody panning
Objavljeno v RUNG: 17.09.2018; Ogledov: 3345; Prenosov: 0
Gradivo ima več datotek! Več...

9.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, vabljeno predavanje na konferenci brez natisa

Ključne besede: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Objavljeno v RUNG: 16.03.2018; Ogledov: 3803; Prenosov: 0
Gradivo ima več datotek! Več...

10.
Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, izvirni znanstveni članek

Opis: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Ključne besede: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Objavljeno v RUNG: 19.10.2017; Ogledov: 4103; Prenosov: 0
Gradivo ima več datotek! Več...

Iskanje izvedeno v 0.06 sek.
Na vrh