1. Database of the Western South Slavic verb HyperVerb 1.0Franc Marušič, Rok Žaucer, Petra Mišmaš, Boban Arsenijević, Marko Simonović, Stefan Milosavljević, Katarina Gomboc, Jelena Simić, 2022, zaključena znanstvena zbirka raziskovalnih podatkov Opis: Slavic, verb,
Ključne besede: Slavic, verb, inflection, theme vowel, Slovenian, BCMS, Bosnian, Croatian, Serbian, verbal morphology, verbal stress pattern, verbal inflection
Objavljeno v RUNG: 12.09.2022; Ogledov: 1022; Prenosov: 43
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2. Unraveling the Role of Major Facilitator Superfamily (MFS) Transporters in Plant Abiotic Stress ToleranceChristina Paparokidou, 2014, končno poročilo o rezultatih raziskav Opis: The yields of worldwide crop production are negatively affected by adverse environmental conditions, such as drought, salinity, heavy metal contamination and nutrient depletion in cultivated soils (Yan et al., 2013; Golldack et al., 2011; Yadav, 2010; Kobayashi and Nishizawa, 2012). This reduced crop production constitutes a major problem for food sustainability world-wide (Spiertz, 2013). Indeed, one of the major challenges for plant biotechnology will be to satisfy the increased demand for food on one hand, and to compensate for the loss of crop production on the other. Thus, the discovery of new plant genes that are able to cope with these conditions is critical and expected not only to elucidate the molecular mechanisms underlying crop abiotic stress, but also to pioneer genetic engineering strategies for improved crop productivity.
The goal of this project was to identify novel genes belonging to the Major Facilitator Superfamily (MFS) of plant membrane transporters with potential major roles in conferring abiotic stress tolerance, namely to heavy metal, osmotic and iron deficiency stress. We selected three Arabidopsis thaliana MFS transporter genes, provisionally called MFS10, MFS11 and MFS12, which based on publicly available microarray data display specific expression patterns suggesting roles in plant tolerance to different abiotic stresses.
RT-PCR analyses showed that in fact the MFS10 gene is highly induced by cadmium (Cd), selenium (Se), salt and mannitol, while the MFS11 gene is upregulated by abscisic acid (ABA) and glucose, and the MFS12 gene by iron (Fe) deficiency, ABA and glucose. Furthermore, subcellular localization of fluorescent reporter fusions indicated that the MFS10 and MFS11 gene products are both plasma membrane localized transporters, while the encoded product of the MFS12 gene appears to be an endoplasmic reticulum localized transporter. Finally, reverse genetics using a null mutant allele for the MFS10 gene demonstrated that it functions as a regulator of plant responses to hyperosmotic (drought and salt) stress.
Ključne besede: Major Facilitator Superfamily (MFS), abiotic stress tolerance, heavy metals, osmotic stress, ABA stress, iron deficiency, T-DNA insertion lines, RT-PCR, confocal microscopy.
Objavljeno v RUNG: 03.05.2022; Ogledov: 1083; Prenosov: 0
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3. Abscisic acid-regulated water channels and their role in plant acclimation and tolerance to water stressChristina Paparokidou, 2014, magistrsko delo Opis: The yield of worldwide crop production has already been negatively affected by high salinity and water deficiency prevailing in many of the cultivated lands (Yan et al., 2013; Serraj et al., 2011; Golldack et al., 2011). Consequently, reduced crop production is a major problem in terms of food sustainability world-wide (Spiertz, 2013). Plants as sessile organisms have to dynamically and constantly cope with various types of stress in their environment. Although stress perception by plants remains elusive (Gan et al., 2010; Shachar-Hill et al., 2013; Wu et al., 2012), the stress-responsive mechanisms that follow are starting to be better understood (Wasilewska et al., 2008; Kohli et al., 2013; Osakabe et al., 2013).
Abscisic acid (ABA) is a central plant hormone produced in response to abiotic stress and has been shown to play important roles in plant acclimation and tolerance towards stress (Nakashima and Yamaguchi-Shinozaki, 2013; Osakabe et al., 2014). ABA accumulation triggers the expression of a plethora of genes within the plant cell (Liu et al., 2013). The ABA-regulated genes are various in nature, including enzymes involved in osmolyte and cell wall biosynthesis, detoxifying enzymes, enzymes for fatty acid metabolism, proteinase inhibitors, macromolecule protective proteins, lipid transfer proteins, ion transporters and water channels, transcription factors (TFs), protein kinases, protein phosphatases and proteinases (Roychoudhury et al., 2013; Rock, 2000).
In this literature review the role of water channel-encoding genes, namely aquaporins (AQPs), in plant water stress will be discussed. The aim of this study is to understand how ABA-regulated AQPs are able to contribute to the plant’s tolerance and acclimation during water stress. The knowledge gained from this study is important for the engineering of salt and drought resistant crops within the modern agricultural context, thus contributing to world’s crop sustainable production (Jacobs et al., 2011; Park et al., 2005; Kujur et al., 2013).
Ključne besede: Abscisic acid (ABA), ABA-regulated genes, aquaporins (AQPs), ABA-regulated AQPs, ABA-dependent water stress, root hydraulic conductance (Lpr), leaf hydraulic conductance (Kleaf), stomatal conductance (gs), AQP bioengineering
Objavljeno v RUNG: 03.05.2022; Ogledov: 1026; Prenosov: 0
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4. Real-time monitoring of Arundo donax response to saline stress through the application of in vivo sensing technologyJanni Michela, Claudia Cocozza, Federico Brilli, Sara Pignattelli, Filippo Vurro, Nicola Coppede, Manuele Bettelli, Davide Calestani, Francesco Loreto, Andrea Zappettini, 2021, izvirni znanstveni članek Opis: One of the main impacts of climate change on agriculture production is the dramatic increase of saline (Na+) content in substrate, that will impair crop performance and productivity. Here we demonstrate how the application of smart technologies such as an in vivo sensor, termed bioristor, allows to continuously monitor in real-time the dynamic changes of ion concentration in the sap of Arundo donax L. (common name giant reed or giant cane), when exposed to a progressive salinity stress. Data collected in vivo by bioristor sensors inserted at two different heights into A. donax stems enabled us to detect the early phases of stress response upon increasing salinity. Indeed, the continuous time-series of data recorded by the bioristor returned a specific signal which correlated with Na+ content in leaves of Na-stressed plants, opening a new perspective for its application as a tool for in vivo plant phenotyping and selection of genotypes more suitable for the exploitation of saline soils.
Ključne besede: Arundo donax, saline stress, vivo sensing technology
Objavljeno v RUNG: 17.12.2021; Ogledov: 1381; Prenosov: 17
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5. High spatial heterogeneity of water stress levels in Refošk grapevines cultivated in Classical KarstFrancesco Petruzzellis, Sara Natale, Luca Bariviera, Alberto Calderan, Alenka Mihelčič, Jan Reščič, Paolo Sivilotti, Katja Šuklje, Klemen Lisjak, Andreja Vanzo, Andrea Nardini, 2022, izvirni znanstveni članek Ključne besede: Classical Karst, water status, drought stress, grape quality, phenolic profile, vitis vinifera
Objavljeno v RUNG: 11.11.2021; Ogledov: 1530; Prenosov: 0
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6. Identification of environmental stress conditions by comparative analysis of peroxidase activity in betulla pendula and pinus sp. : Group project reportPatrik Cingerli, Katarina Erker, Nina Žvab-Pernat, Darian Rampih, Miroslav Štrbac, Hena Zukić, Kenan Kapetanović, 2021, elaborat, predštudija, študija Opis: The study investigated the possibility of developing a biomarker to evaluate the stress level in trees. The analyses have been performed on tree samples appertaining to two different tree species and collected at two locations in Slovenia. The presence and the activity of the proteins – peroxidases was measured by the qualitative method of the agarose gel electrophoresis and the quantitative method of spectrometry. It was found out that by measuring the presence of the biomarkers and pigments in the tree samples we can determine the stress levels and thus the health status of trees.
Ključne besede: biomarkers, environmental stress, pigments, peroxidase
Objavljeno v RUNG: 29.09.2021; Ogledov: 1569; Prenosov: 0
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9. Short-term physiological and biometrical responses of Lepidium sativum seedlings exposed to PET-made microplastics and acid rainSara Pignattelli, Andrea Broccoli, Manuela Piccardo, Serena Felline, Antonio Terlizzi, Monia Renzi, 2021, izvirni znanstveni članek Opis: Plastics enter in terrestrial natural system primarily by agricultural purposes, while acid rain is the result of
anthropogenic activities. The synergistic effects of microplastics and acid rain on plant growth are not known. In
this study, different sizes of polyethylene terephthalate (PET) and acid rain are tested on Lepidium sativum, in two
separate experimental sets. In the first one we treated plants only with PET, in the second one we used PET and
acid rain together. In both experimentations we analyzed: i) plant biometrical parameters (shoot height, leaf
number, percentage inhibition of seed germination, fresh biomass), and ii) oxidative stress responses (hydrogen
peroxide; ascorbic acid and glutathione). Results carried out from our experiments highlighted that different
sizes of polyethylene terephthalate are able to affect plant growth and physiological responses, with or without
acid rain supplied during acute toxicity (6 days).
Short description: This study showed that different sizes of PET microplastics affect physiological and biometrical
responses of Lepidum sativum seedlings, with or without acid rain; roots and leaves responded differently
Ključne besede: Polyethylene terephthalate
Acid rain
Oxidative stress
Biometrical response
Lepidium sativum
Objavljeno v RUNG: 16.12.2020; Ogledov: 2020; Prenosov: 0
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