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Thermal lens spectrometric monitoring of photocatalytic reduction of Cr(VI)
Mohanachandran Nair Sindhu Swapna, Lučka Godec, Dorota Korte, Mladen Franko, 2024, objavljeni povzetek znanstvenega prispevka na konferenci

Ključne besede: thermal lens, photocatalytic reduction, chromium VI
Objavljeno v RUNG: 17.09.2024; Ogledov: 1111; Prenosov: 3
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Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertation
Gbenga Folorunsho Oginni, 2024, doktorska disertacija

Opis: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Ključne besede: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Objavljeno v RUNG: 10.09.2024; Ogledov: 1342; Prenosov: 21
.pdf Celotno besedilo (3,80 MB)

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Thermal lens technique : a novel tool for the detection and quantification of microplastics in water
P. Anju Abraham, Mohanachandran Nair Sindhu Swapna, Sankaranarayana Iyer Sankararaman, 2023, objavljeni povzetek znanstvenega prispevka na konferenci

Ključne besede: thermal lens, microplastics
Objavljeno v RUNG: 09.01.2024; Ogledov: 2354; Prenosov: 5
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