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Computational design of customised nanobodies for biotechnological applications
Sara Fortuna, Ario de Marco, Miguel Soler, 2016, prispevek na konferenci brez natisa

Opis: In silico modeling to improve the biophysical characteristics of recombinant single-domain antibodies
Najdeno v: osebi
Ključne besede: nanobodies, modeling, protein stability, antibody humanization, molecular dynamics
Objavljeno: 26.04.2016; Ogledov: 2869; Prenosov: 0
.pdf Polno besedilo (2,42 MB)

Lo sviluppo biotecnologico della pallottola magica proposta nel 1906 da Paul Ehrlich, padre della chemioterapia
Ario de Marco, predavanje na tuji univerzi

Opis: Description of Paul Ehrlich contribution to modern medicine
Najdeno v: osebi
Ključne besede: targeted therapy, antibodies, biomarkers, molecule functionalization, differential staining
Objavljeno: 26.04.2016; Ogledov: 2933; Prenosov: 0
.pdf Polno besedilo (4,05 MB)

Selection criteria and engineering of nanobodies for oncological applications
Ario de Marco, predavanje na tuji univerzi

Opis: Lecture relative to the biotechnological features of nanobodies
Najdeno v: osebi
Ključne besede: nanobodies, phage display, antibody engineering, panning
Objavljeno: 14.07.2016; Ogledov: 2690; Prenosov: 0
.pdf Polno besedilo (22,12 KB)

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Jean-Christophe Rain, Laetitia Ligat, Ario de Marco, Emilie Lemesre, Laura Keller, Virginie Bernard, Nicolas Bery, Sandrine Moutel, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, izvirni znanstveni članek

Opis: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Najdeno v: osebi
Ključne besede: nanobodies, synthetic phage display library, in vitro panning
Objavljeno: 17.08.2016; Ogledov: 3082; Prenosov: 192
.pdf Polno besedilo (4,32 MB)

A Nano-Imunoassay based on fluorescence and atomic force spectroscopy for the detection of circulating cancer biomarkers: The case of HER2 positive breast cancer
Ario de Marco, Alessandro Bosco, Pietro Parisse, Elena Ambrosetti, Loredana Casalis, Elsa Tagliabue, 2016, objavljeni povzetek znanstvenega prispevka na konferenci

Opis: A promising new approach for the detection of biomarkers in patient sera
Najdeno v: osebi
Ključne besede: AFM, diagnostic antibodies, HER2
Objavljeno: 17.08.2016; Ogledov: 2759; Prenosov: 0
.pdf Polno besedilo (854,47 KB)
Gradivo ima več datotek! Več...

Molecular dynamics simulations and docking enable to explore the biophysical factors controlling the yields of engineered nanobodies
Ario de Marco, Miguel Soler, Sara Fortuna, 2016, izvirni znanstveni članek

Opis: Nanobodies (VHHs) have proved to be valuable substitutes of conventional antibodies for molecular recognition. Their small size represents a precious advantage for rational mutagenesis based on modelling. Here we address the problem of predicting how Camelidae nanobody sequences can tolerate mutations by developing a simulation protocol based on all-atom molecular dynamics and wholemolecule docking. The method was tested on two sets of nanobodies characterized experimentally for their biophysical features. One set contained point mutations introduced to humanize a wild type sequence, in the second the CDRs were swapped between single-domain frameworks with Camelidae and human hallmarks. The method resulted in accurate scoring approaches to predict experimental yields and enabled to identify the structural modifications induced by mutations. This work is a promising tool for the in silico development of single-domain antibodies and opens the opportunity to customize single functional domains of larger macromolecules
Najdeno v: osebi
Ključne besede: nanobodies, molecular dynamics, modeling, antibody solubility
Objavljeno: 11.10.2016; Ogledov: 2946; Prenosov: 198
.pdf Polno besedilo (1,95 MB)

Nanobodies for research & clinical applications
Ario de Marco, predavanje na tuji univerzi

Opis: Nanobody technology evolved rapidly in the last years and now is available for applications in basic sciences as well as in diagnostics and therapy
Najdeno v: osebi
Ključne besede: nanobodies, antibody functionalization, phage display panning, recombinant antibody production
Objavljeno: 11.10.2016; Ogledov: 3086; Prenosov: 0
.pdf Polno besedilo (773,47 KB)

Nanobody technology: principles & applications
Ario de Marco, vabljeno predavanje na konferenci brez natisa

Najdeno v: osebi
Ključne besede: nanobodies, nanotechnologies, biomarker identification
Objavljeno: 29.03.2017; Ogledov: 2770; Prenosov: 0
.pdf Polno besedilo (114,69 KB)

Assessment of the actual antibody-antigen binding conditions at the surface of biological membranes
Ario de Marco, 2016, objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje)

Najdeno v: osebi
Ključne besede: Exosomes, avidity effect, AFM
Objavljeno: 30.11.2016; Ogledov: 3048; Prenosov: 0
.pdf Polno besedilo (211,28 KB)
Gradivo ima več datotek! Več...

Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Ronan Crepin, David Gentien, Angeline Duche, Audrey Rapinat, Cecile Reyes, Fariba Nemati, Gerald Massonnet, Didier Deacaudin, Selma Djander, Sandrine Moutel, Klervi Even Desrumeaux, Nathalie Cassoux, Sophie Piperno-Neumann, Sebastian Amigorena, Franck Perez, Sergio Roman-Roman, Ario de Marco, 2017, izvirni znanstveni članek

Opis: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Najdeno v: osebi
Ključne besede: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Objavljeno: 19.04.2017; Ogledov: 3776; Prenosov: 0
.pdf Polno besedilo (1,11 MB)

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