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Opis: The current 30% of canine perinatal mortality (Tonnessen et al., 2012) claims the need to deepen the knowledge about this phase, characterized by long-term hormonal and metabolic changes, that could benefit of long time-frame methods of investigation, reducing the quantity of samplings and thus respecting animal welfare (Veronesi et al., 2015). Indeed, apart from the well-known invasiveness of blood samplings, the necessary repeated collection of feces, urine and saliva, providing punctual information, could be stressful for the newborns and their mothers with negative effects on health status and maternal behavior. Given that sexual steroid hormones were reported to have an influence on health outcomes and development already from the perinatal period (Frey et al., 2017), in this work 17-β-Estradiol (E2) and Testosterone (T) concentrations were assessed from the claws of dogs up to 60 days of age, to identify possible endogenous biomarkers. Ten male and 10 female puppies, viable and healthy, born by elective cesarean section, were enrolled. Samplings were performed by trimming claws at birth, and the regrowth at 30 and 60 days of age after the breeder or the owner signed an informed consent. Then, E2 and T concentrations were analyzed by RIA (Veronesi et al., 2015) and a possible effect of gender evaluated by ANOVA. All the hormonal claws concentrations showed a significant (p<0.001) drop from birth to 30 and 60 days of age, while no significant changes were observed between 30 and at 60 days of age (Table 1). No influence of the newborns’ gender was found. Because of the higher levels of E2 and T observed at birth and at 30 days of age, it could be supposed that a source of production could be the placental and maternal compartments. However, the direct involvement of the fetus itself could not be excluded, given the reported accumulation from the nail capillary bed of those hormones (de Berker et al., 2007) and the production of sexual steroid hormones by feline fetal gonads (Braun and Jewgenow, 2017).
Ključne besede: Dog, Puppy, Claws, 17-ß-Estradiol, Testosterone.
Objavljeno v RUNG: 09.04.2019; Ogledov: 3236; Prenosov: 0
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Opis: This research explores the general and actual accessibility to cultural spaces and organizations, focusing on the network of relationships between contents, innovation, and participation. It investigates how Culture can be perceived as a normal experience, actually able to set a rich dialogue with each of us, normal ordinary people/consumers. Normality means inclusion and sharing. The proposed investigation is based on a triad of interactions among culture, economics, and design. Assuming that Culture generates its value from a cognitive approach, or from a dynamic and context-dependent value chain that is subject to a cognitive evolution, this research acknowledges that the cultural experience is subject to a double issue of access: - The horizontal question is related to complications associated with the structure. Believing that ‘culture is special’ implies the risk of progressively widening the gap between cultural supply and society. Culture is ‘locked’ in specific sites and a big slice of the world’s adult population has yet to be involved in any cultural experience. - Once physically inside the cultural structure, the vertical issue is generated by the difficulty in entering in contact with the offered contents. This research focuses only on museums and multimedia exhibitions in which the learning process has changed: the research presents itself as a conversation where both ‘those who know’ and ‘those who learn’ play equally active parts in a relative process of understanding. Starting from the existing processes, forms, previous studies and case studies, the survey yearns for their systematization in innovative models. The process, from theory to practice and vice versa, goes beyond a traditional mechanism of deduction: it moves from specific contexts to the abstraction of replicable approaches. The question of how the narration emerges guides you toward a new method of analysis, study, and cataloguing; a schematization capable of investigating not only knowledge, but also the visitor's ‘cognitive metabolism’ (how knowledge is acquired) during the multimedia experience; an innovative multipurpose tool, useful for both the museum institution and the designer. The research finally assesses and takes into account an actual experiment, the outcomes of which may prove to be useful in feeding theoretical implications with empirical experiences: RovelloDue - Piccolo Spazio Politecnico, a temporary multimedia space.
Ključne besede: audience development, cognitive accessibility, cultural addiction, culture, human, emotion, immersion, innovation, interactive exhibitions, participation, natural interfaces, normality, technologies
Objavljeno v RUNG: 13.12.2018; Ogledov: 3566; Prenosov: 63
Celotno besedilo (45,89 MB)

Opis: Arsenic contamination in natural water is a worldwide problem and a major health concern. In master thesis hydrogeochemistry and toxicity effects of natural waters rich in arsenic were studied. The main objective of the master thesis was to evaluate As pollution of the Freixeda stream and groundwater in abandoned Freixeda gold mine area in NE Portugal near Mirandela city (41.413767 N 7.103562 W) and compare it with the data from previous studies. Further on, toxicological evaluation of selected water samples was performed in the in vitro system of human cell line Caco-2. Chemical analyses of sampled water samples with use of different modelling sofware show that groundwater have higher sulphate and bicarbonate values than surface water, which could be the reason for As desorption and higher As values in groundwater. Water-rock interaction promotes reduction and dissolution of sulphide minerals and in reductive environments dissolution of secondary Fe minerals releases adsorbed As into solution. Toxicological testing on human cells included cytotoxicity assay, genotoxicity assay and production of reactive oxigene species (ROS). Genotoxicity was only modestly affected by a short-term exposure to As-contaminated water samples, however, higher concentrations of As in real samples lead to higher level of oxidative stress and decreased cell viability. Exposure of cells to pure As(III) solution show clear concentration dependent decrease in cell metabolism and viability, strong genotoxicity and increased ROS generation. Considering the worldwide extent of As contamination in natural waters and ability of intestinal epithelium to reduce the potential harmful effects of As, more studies evaluating the human intestinal permeability for As should be done in the future.
Ključne besede: Water quality, arsenic, hydrogeochemistry, toxicity, Caco-2 cell line
Objavljeno v RUNG: 26.09.2018; Ogledov: 5439; Prenosov: 197
Celotno besedilo (3,76 MB)

Ključne besede: CTA, array calibration, groups of telescopes
Objavljeno v RUNG: 16.02.2018; Ogledov: 2893; Prenosov: 149
Celotno besedilo (702,76 KB)

Opis: During the last fetal stage of development and in the neonatal period, the hypothalamic- pituitary- adrenal (HPA) axis secretes cortisol (C), responsible for several physiologic processes. The claws were recently proved to be a useful, non- invasive matrix for long time- frame retrospective C levels analysis also in puppies (Veronesi et al. 2015, Theriogenology 84:791–796). Because of the scarce knowledge on canine perinatology, this study was aimed to assess the C concentrations in claws of newborn puppies collected at birth, 30 and 60 days of age, and to evaluate the possible influence of age, gender and type of birth on C claws accumulation. The study was performed on 58 large purebred, normal, healthy puppies, born by spontaneous or cesarean parturition. The mean C claws concentrations significantly decrease from birth (26 ± 16.39 pg/mg) to 30 (10 ± 4.53 pg/mg) and 60 (6 ± 3.12 pg/mg) days of age (p < 0.0001); a decrease was also seen from 30 to 60 days of age (p < 0.05). No influence of newborn gender and type of birth was found. This trend of decrease is in agreement with data reported by (Veronesi et al. 2015, Theriogenology 84:791–796) in dead puppies, and suggests the important role of C around the time of birth. However the exact source of the C accumulated in claws of puppies at birth must be clarified, while the source of C accumulated at 30, and even more at 60 days, can be supposed to be addressed to the newborn itself.
Ključne besede: Cortisol, claws, dog
Objavljeno v RUNG: 01.09.2017; Ogledov: 4650; Prenosov: 0
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