1. Metabolic dysregulation in vitamin E and carnitine shuttle energy mechanisms associate with human frailtyJames Nazroo, Royston Goodacre, Neil Pendleton, Frederick Wu, Iain White, Bram Vanhoutte, Gindo Tampubolon, Alan Marshall, Krisztina Mekli, Zahra Rattray, Tarani Chandola, Caroline Johnson, Drupad Trivedi, Yun Xu, Nicholas Rattray, 2019, izvirni znanstveni članek Opis: Global ageing poses a substantial economic burden on health and social care costs. Enabling a greater proportion of older people to stay healthy for longer is key to the future sustainability of health, social and economic policy. Frailty and associated decrease in resilience plays a central role in poor health in later life. In this study, we present a population level assessment of the metabolic phenotype associated with frailty. Analysis of serum from 1191 older individuals (aged between 56 and 84 years old) and subsequent longitudinal validation (on 786 subjects) was carried out using liquid and gas chromatography-mass spectrometry metabolomics and stratified across a frailty index designed to quantitatively summarize vulnerability. Through multivariate regression and network modelling and mROC modeling we identified 12 significant metabolites (including three tocotrienols and six carnitines) that differentiate frail and non-frail phenotypes. Our study provides evidence that the dysregulation of carnitine shuttle and vitamin E pathways play a role in the risk of frailty.
Najdeno v: osebi
Ključne besede: metabolomics, frailty, ageing, LC-MS, serum
Objavljeno: 08.11.2019; Ogledov: 2535; Prenosov: 85
 Polno besedilo (2,35 MB) |
2. Untargeted molecular analysis of exhaled breath as a diagnostic test for ventilator-associated lower respiratory tract infections (BreathDx)Murtaza Bulut, Waqar Ahmed, Antonio Artigas, Iain R. White, Hugo Knobel, Timothy Felton, Nicholas J. W. Rattray, Oluwasola Lawal, Pouline M. van Oort, Tamara M. E. Nijsen, 2021, kratki znanstveni prispevek Opis: Patients suspected of ventilator-associated lower respiratory tract infections (VA-LRTIs) commonly receive broad-spectrum antimicrobial therapy unnecessarily. We tested whether exhaled breath analysis can discriminate between patients suspected of VA-LRTI with confirmed infection, from patients with negative cultures. Breath from 108 patients suspected of VA-LRTI was analysed by gas chromatography-mass spectrometry. The breath test had a sensitivity of 98% at a specificity of 49%, confirmed with a second analytical method. The breath test had a negative predictive value of 96% and excluded pneumonia in half of the patients with negative cultures. Trial registration number: UKCRN ID number 19086, registered May 2015.
Najdeno v: osebi
Ključne besede: ventilator-associated pneumonia, breath analysis, volatile organic compounds, metabolomics, intensive care, hospital acquired infections
Objavljeno: 07.09.2021; Ogledov: 1343; Prenosov: 0
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3. Breath and plasma metabolomics to assess inflammation in acute strokeCraig Johnson, Nicholas J. W. Rattray, Amit K. Kishore, Royston Goodacre, Waqar Ahmed, Iain R. White, Maxim Wilkinson, Craig J. Smith, Stephen J. Fowler, 2021, izvirni znanstveni članek Opis: Inflammation is strongly implicated in both injury and repair processes occurring after stroke. In this exploratory study we assessed the feasibility of repeated sampling of exhaled volatile organic compounds and performed an untargeted metabolomic analysis of plasma collected at multiple time periods after stroke. Metabolic profiles were compared with the time course of the inflammatory markers C-reactive protein (CRP) and interleukin-6 (IL-6). Serial breath sampling was well-tolerated by all patients and the measurement appears feasible in this group. We found that exhaled decanal tracks CRP and IL-6 levels post-stroke and correlates with several metabolic pathways associated with a post-stroke inflammatory response. This suggests that measurement of breath and blood metabolites could facilitate development of novel therapeutic and diagnostic strategies. Results are discussed in relation to the utility of breath analysis in stroke care, such as in monitoring recovery and complications including stroke associated infection.
Najdeno v: osebi
Ključne besede: stroke, metabolomics, breath, VOCs, inflammation
Objavljeno: 18.11.2021; Ogledov: 1068; Prenosov: 58
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