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Biotransformation of copper oxide nanoparticles by the pathogenic fungus Botrytis cinereaEva Kovačec,
Marjana Regvar,
Johannes Teun van Elteren,
Iztok Arčon,
Tamás Papp,
Darko Makovec,
Katarina Vogel-Mikuš, 2017, original scientific article
Abstract: Two plant pathogenic fungi, Botrytis cinerea and Alternaria alternata, isolated from crop plants, were
exposed to Cu in ionic (Cu2þ), microparticulate (MP, CuO) or nanoparticulate (NP, Cu or CuO) form, in
solid and liquid culturing media in order to test fungal response and toxic effects of the mentioned
compounds for the potential use as fungicides. B. cinerea has shown pronounced growth and lower levels
of lipid peroxidation compared to A. alternata. Its higher resistance/tolerance is attributed mainly to
biotransformation of CuO and Cu NPs and CuO MPs into a blue compound at the fungal/culturing media
interface, recognized by Cu K-edge EXAFS analysis as Cu-oxalate complex. The pronounced activity of
catechol-type siderophores and organic acid secretion in B. cinerea induce leaching and mobilization of
Cu ions from the particles and their further complexation with extracellularly secreted oxalic acid. The
ability of pathogenic fungus to biotransform CuO MPs and NPs hampers their use as fungicides. However
the results show that B. cinerea has a potential to be used in degradation of Cu(O) nanoparticles in
environment, copper extraction and purification techniques.
Keywords: copper, metal oxide nanoparticles, detoxification mechanisms, metal pollution, Cu-oxalate
Published in RUNG: 23.08.2017; Views: 5689; Downloads: 0
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