1. Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidaseClaudia D'Ercole, Rossella Svigelj, Tanja Mrak, Ario De Marco, 2025, izvirni znanstveni članek Opis: Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees.
Ključne besede: plant stress, scavengers, diagnostics, nanobodies, biosensors
Objavljeno v RUNG: 11.04.2025; Ogledov: 183; Prenosov: 2
 Celotno besedilo (4,65 MB)
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2. Metagenomics studies in aquaculture systems : big data analysis, bioinformatics, machine learning and quantum computingOrkid Coskuner-Weber, Semih Alpsoy, Ozgur Yolcu, Egehan Teber, Ario De Marco, Spase Shumka, 2025, pregledni znanstveni članek Ključne besede: marine biology, machine learning, diagnostics
Objavljeno v RUNG: 07.04.2025; Ogledov: 239; Prenosov: 0
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3. The peppermint breath test: a benchmarking protocol for breath sampling and analysis using GC-MSMaxim Wilkinson, Iain R. White, Katie Hamshere, Olaf Holz, Sven Schuchardt, Francesca G. Bellagambi, Tommaso Lomonaco, Denise Biagini, Laura Di Francesco, Stephen J. Fowler, 2020, izvirni znanstveni članek Opis: Exhaled breath contains hundreds of volatile organic compounds (VOCs) which offers the potential for diagnosing and monitoring a wide range of diseases. As the breath research field has grown, sampling and analytical practices have become highly varied between groups. Standardisation would allow meta-analyses of data from multiple studies and greater confidence in published results. The Peppermint Consortium has been formed to address this task of standardisation. In the current study we aimed to generate initial benchmark values for thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) analysis of breath samples containing peppermint-derived VOCs. Headspace analysis of peppermint oil capsules was performed to determine compounds of interest. Ten healthy participants were recruited by three groups. Each participant provided a baseline breath sample prior to taking a peppermint capsule, with further samples collected at 60, 90, 165, 285 and 360 min following ingestion. Sampling and analytical protocols were different for each institution, in line with their usual practice. Samples were analysed by TD-GC-MS and benchmarking values determined for the time taken for detected peppermint VOCs to return to baseline values. Sixteen compounds were identified in the capsule headspace. Additionally, 2,3-dehydro-1,8-cineole was uniquely found in the breath samples, with a washout profile that suggested it was a product of peppermint metabolism. Five compounds (α-pinene, β-pinene, eucalyptol, menthol and menthone) were quantified by all three groups. Differences in recovery were observed between the groups, particularly for menthone and menthol. The average time taken for VOCs to return to baseline was selected as the benchmark and were 441, 648, 1736, 643 and 375 min for α-pinene, β-pinene, eucalyptol, menthone and menthol respectively. An initial set of easy-to-measure benchmarking values for assessing the performance of TD-GC-MS systems for the analysis of VOCs in breath is presented. These values will be updated when more groups provide additional data.
Ključne besede: Volatile organic compounds, breath, diagnostics, standardisation
Objavljeno v RUNG: 11.12.2020; Ogledov: 5282; Prenosov: 0
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5. A compact nanobody-DNAzyme conjugate enables antigen detection and signal amplificationGiulio Bernardinelli, Sandra Oloketuyi, Franziska Werner, Elisa Mazzega, Bjorn Hogberg, Ario De Marco, 2020, izvirni znanstveni članek Ključne besede: DNAzyme, nanobodies, diagnostics, A. minutum
Objavljeno v RUNG: 10.11.2019; Ogledov: 4626; Prenosov: 0
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9. Urinary Extracellular Vesicle Biomarkers in Urological Cancers: From Discovery Towards Clinical ImplementationBert Dhondt, Jan Van Deun, Silke Vermaerke, Ario De Marco, Nicolaas Lumen, Olivier De Wever, An Hendrix, 2018, pregledni znanstveni članek Ključne besede: Extracellular vesicles, cancer biomarkers, diagnostics
Objavljeno v RUNG: 04.05.2018; Ogledov: 5266; Prenosov: 0
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