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1.
Harnessing in vitro selection opportunities for obtaining epitope-specific binders : lecture at the CDKL5 Forum 2024, Cambridge, MA, USA, 27 October 2024
Ario De Marco, 2024, prispevek na konferenci brez natisa

Ključne besede: CDKL5, nanobodies, biopanning
Objavljeno v RUNG: 05.11.2024; Ogledov: 238; Prenosov: 0
Gradivo ima več datotek! Več...

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Recombinant antibodies
Ario De Marco, 2024, samostojni znanstveni sestavek ali poglavje v monografski publikaciji

Ključne besede: antibody fragments, nanobodies, recombinant expression
Objavljeno v RUNG: 20.09.2024; Ogledov: 525; Prenosov: 2
URL Povezava na datoteko
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3.
Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertation
Gbenga Folorunsho Oginni, 2024, doktorska disertacija

Opis: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Ključne besede: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Objavljeno v RUNG: 10.09.2024; Ogledov: 595; Prenosov: 11
.pdf Celotno besedilo (3,80 MB)

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Imagination is the limit for nanobody applications : lecture at the Zhejiang Lab for Regenerative Medicine, Vision and Brain Health, Zhejiang, March 21st 2024
Ario De Marco, 2024, predavanje na tuji univerzi

Ključne besede: nanobodies
Objavljeno v RUNG: 25.03.2024; Ogledov: 1258; Prenosov: 0
Gradivo ima več datotek! Več...

8.
Implications of nanobody library design : lecture at the Zhejiang University, March 22nd 2024
Ario De Marco, 2024, predavanje na tuji univerzi

Ključne besede: nanobodies
Objavljeno v RUNG: 25.03.2024; Ogledov: 975; Prenosov: 0
Gradivo ima več datotek! Več...

9.
The long march of nanobodies : from freak elements to biotechnology stars
Ario De Marco, 2024, prispevek na konferenci brez natisa

Ključne besede: nanobodies
Objavljeno v RUNG: 25.03.2024; Ogledov: 1156; Prenosov: 0
Gradivo ima več datotek! Več...

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