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6. Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertationGbenga Folorunsho Oginni, 2024, doktorska disertacija Opis: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Ključne besede: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Objavljeno v RUNG: 10.09.2024; Ogledov: 620; Prenosov: 11
 Celotno besedilo (3,80 MB) |
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10. Eco-conscious nanofluids : exploring heat transfer performance with graphitic carbon nitride nanoparticlesVijayakumar Gokul, Mohanachandran Nair Sindhu Swapna, Sankaranarayana Iyer Sankararaman, 2023, izvirni znanstveni članek Opis: Abstract
The work explores the heat transfer capabilities of semiconducting graphitic carbon nitride (g-C3N4) nanofluids. Also, it presents a sustainable and eco-friendly method for synthesizing g-C3N4 nanoparticles using commercially available rice flour as a natural carbon precursor through hydrothermal treatment. The synthesized sample subjected to various characterizations, including analysis of their structure, morphology, thermal properties, and optical properties. The optical bandgap (2.66 eV) is deduced through Tauc plot analysis and reveals the semiconducting nature of the sample. The formation of g-C3N4 is confirmed by various spectroscopic techniques, including X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR), and Raman spectroscopy. Thermogravimetric analysis (TGA) demonstrates the nanoparticles’ excellent thermal stability up to 550 °C, indicating potential applications in heat transfer fluids. The investigation of concentration-dependent thermal diffusivity variation using the sensitive mode mismatched dual beam thermal lens technique highlights the potential of g-C3N4 semiconductor nanofluid as an organic and metal-free additive in industry-demanding coolant applications.
Ključne besede: thermal lens, nanofluids
Objavljeno v RUNG: 05.01.2024; Ogledov: 1717; Prenosov: 6
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