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Naslov:Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serum
Avtorji:ID Martelanc, Mitja, Univerza v Novi Gorici (Avtor)
ID Žiberna, Lovro, Univerza v Trstu (Avtor)
ID Passamonti, Sabina, Univerza v Trstu (Avtor)
ID Franko, Mladen, Univerza v Novi Gorici (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:We present the applicability of a new ultra sensitive analytical method for the simultaneous determination of biliverdin and bilirubin in human serum. The method comprises isocratic reversed-phase(RP) C18 high-performance liquid chromatography (HPLC) and thermal lens spectrometric detection (TLS)based on excitation by a krypton laser emission line at 407 nm. This method enables the separation of IX-α biliverdin and IX-α bilirubin in 11 min. with limit of detection (LOD) and limit of quantitation (LOQ) for biliverdin of 1.2 nM and 3 nM, and 1nM and 2.8 nM for bilirubin,respectively.In addition, a step-gradient elution was set up, by changing the mobile phase composition, in order to further enhance the sensitivity for bilirubin determination with LOD and LOQ of 0.5 nM and 1.5 nM, respectively. In parallel, an isocraticHPLC-DAD method was developed for benchmarking against HPLC-TLS methods. The LOD and LOQ forbiliverdin were 6 nM and 18 nM, and 2.5 nM and 8nM for bilirubin,respectively. Additionally, both isocratic methods were applied for measuring biliverdin and free bilirubin in human serum samples (from 2 male and 2 female healthy donors). Combining isocratic HPLC method with TLS detector was crucial for first ever biliverdin determination in serum together with simultaneous free bilirubin determination. We showed for the first time the concentration ratio of free bilirubin versus unbound biliverdin in human serum samples.
Ključne besede:Bilirubin, biliverdin, serum, HPLC, TLS
Verzija publikacije:Recenzirani rokopis
Leto izida:2016
Št. strani:7
Številčenje:154
PID:20.500.12556/RUNG-2220 Novo okno
COBISS.SI-ID:4178683 Novo okno
DOI:10.1016/j.talanta.2016.03.053 Novo okno
NUK URN:URN:SI:UNG:REP:9HIVYATN
Datum objave v RUNG:29.03.2016
Število ogledov:7425
Število prenosov:0
Metapodatki:XML DC-XML DC-RDF
:
MARTELANC, Mitja, ŽIBERNA, Lovro, PASSAMONTI, Sabina in FRANKO, Mladen, 2016, Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serum. Talanta [na spletu]. 2016. Vol. 154. [Dostopano 23 marec 2025]. DOI 10.1016/j.talanta.2016.03.053. Pridobljeno s: https://repozitorij.ung.si/IzpisGradiva.php?lang=slv&id=2220
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Gradivo je del revije

Naslov:Talanta
Skrajšan naslov:Talanta
Založnik:Elsevier
Leto izida:2016
ISSN:0039-9140

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