Repozitorij Univerze v Novi Gorici

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Naslov:Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Avtorji:ID Crepin, Ronan, Institut Curie (Avtor)
ID Veggiani, Gianluca, Institut Curie (Avtor)
ID Djender, Selma, Institut Curie (Avtor)
ID Beugnet, Anne, Institut Curie (Avtor)
ID Planeix, Francois, Institut Curie (Avtor)
ID Pichon, Christophe, Institut Curie (Avtor)
ID Moutel, Sandrine, Institut Curie (Avtor)
ID Amigorena, Sebastian, Institut Curie (Avtor)
ID Pérez, Franck, Institut Curie (Avtor)
ID Ghinea, Nicolae, Institut Curie (Avtor)
ID De Marco, Ario, UNG (Avtor), et al.
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:UNG - Univerza v Novi Gorici
Opis:Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Ključne besede:nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Verzija publikacije:Objavljena publikacija
Leto izida:2017
Št. strani:1567-1572
Številčenje:493
PID:20.500.12556/RUNG-3322-b86c7cca-992c-0d39-70bc-b8e3cc19c7ad Novo okno
COBISS.SI-ID:4933627 Novo okno
NUK URN:URN:SI:UNG:REP:4WYPVXVK
Datum objave v RUNG:19.10.2017
Število ogledov:3974
Število prenosov:0
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Gradivo je del revije

Naslov:Biochemical Biophysical Research Communications
ISSN:0006-291X

Licence

Licenca:CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
Opis:Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
Začetek licenciranja:19.10.2017

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