Repozitorij Univerze v Novi Gorici

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Naslov:Nanobodies: towards rational design of immune-reagents
Avtorji:ID De Marco, Ario, Uiversity of Nova Gorica (Avtor)
Datoteke: Gradivo nima datotek, ki so prostodostopne za javnost. Gradivo je morda fizično dosegljivo v knjižnici fakultete, zalogo lahko preverite v COBISS-u. Povezava se odpre v novem oknu
Jezik:Angleški jezik
Vrsta gradiva:Delo ni kategorizirano
Tipologija:1.10 - Objavljeni povzetek znanstvenega prispevka na konferenci (vabljeno predavanje)
Organizacija:UNG - Univerza v Novi Gorici
Opis:Antibodies are irreplaceable reagents in both research and clinical practice. Despite their relevance, the structural complexity of conventional mono- and polyclonal antibodies (IgG) has always been a limit for their engineering towards reagents optimized for specific applications, such as in vivo diagnostics and therapy. Furthermore, their isolation is time consuming, their production expensive, and their functionalization results often in heterogeneous macromolecule populations. These drawbacks promoted the search for both innovative antibody isolation strategies and alternative scaffolds. In vitro panning of pre-immune collections of recombinant antibody fragments allows for the simple and fast recovery of binders. Since they did not undergo somatic maturation, their affinity for targets can be insufficient but on the other hand they can be rapidly mutated by standard molecular biology techniques to generate second-generation antibodies among which to identify clones with improved characteristics. Both stochastic and rational methods have been proposed for the optimization process. Random mutagenesis followed by panning at stringent conditions has been successful used to select binders with improved physical characteristics. Rational methods try to identify in silico key residues involved in the regulation of specific antibody features, such as stability or binding affinity. The accuracy of these methods usually depends on the calculation resources. In this perspective, smaller molecules can be analyzed “better” than larger because of their restricted number of residues. Nanobodies small dimensions have been long appreciated since enable better tissue penetration, shorter clearance time, higher yields. Now it becomes evident that this characteristic makes them also optimal objects for modeling.
Ključne besede:recombinant antibody modeling, nanobody engineering, molecular dynamics and docking
Leto izida:2017
PID:20.500.12556/RUNG-3373-34a806f7-f211-6829-b527-cd5d37c7e282 Novo okno
COBISS.SI-ID:4954619 Novo okno
NUK URN:URN:SI:UNG:REP:FOUFFON5
Datum objave v RUNG:21.03.2018
Število ogledov:4649
Število prenosov:0
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Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Gradivo je del monografije

Naslov:Serbian Biochemical Society - Seventh Conference : Biochemistry of Control in Life and Technology
Uredniki:Ivan Spasojevic
Kraj izida:Belgrade
Leto izida:2017
Prireditelj konference:Serbian Biochemical Society

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